里氏木霉液体发酵法生产纤维素酶

通过比较几株霉菌产纤维素酶活力，发现里氏木霉ＲｕｔＣ－３０活力最高；采用Ａｖｉｃｅｌ与麸皮复合碳源，以及使用ＫＨ２ＰＯ４－Ｋ２ＨＰＯ４缓冲系统控制发酵液ｐＨ，２８℃摇瓶发酵６ｄ，最高酶活达到ＣＭＣａｓｅ１００－１２５Ｕ／ｍｌ，ＦＰＡ９－１２Ｕ／ｍｌ。采用２．５升发酵罐培养，通过控制ｐＨ和溶氧，纤维素酶活力为ＣＭＣａｓｅ１３３．４Ｕ／ｍｌ，ＦＰＡ１１．６７Ｕ／ｍｌ。用硫铵盐析法提取制得纤维素酶干粉，其活力为ＣＭＣａｓｅ３０７４．９Ｕ／ｇ，ＦＰＡ１６６．７Ｕ／ｇ。

The Cellulase Production by Trichoderma reesei in Submerged Fermentation

By comparing cellulase activity of different strains, Trichoderma reesei Rut C-30 was found to be the best one. A combination of Avicel and wheat bran was used as carbon source, as well the KH 2PO 4-K 2HPO 4 buffer system was used for pH control, resulted in very high enzyme production in shake flask culture, Maximum CMCase 100-125U/ml, FPA 9-12U/ml were achieved within 6 days culture. When it was grown in 2.5L fermentor, where pH and DO levels were controlled, the enzyme activity were 133.4U/ml(CMCase)and 11.67U/ml(FPA),respectively. Dried cellulase powder was prepared by (NH 4) 2SO 4 precipitation method, showed 3074.9U/g of CMCase activity and 166.7U/g of FP activity.