壳聚糖酶高产菌株选育及发酵条件研究 |
| |
引用本文: | 陈小娥,夏文水,余晓斌.壳聚糖酶高产菌株选育及发酵条件研究[J].食品与发酵工业,2004,30(3):66-69. |
| |
作者姓名: | 陈小娥 夏文水 余晓斌 |
| |
作者单位: | 1. 江南大学食品学院,无锡,214036;浙江海洋学院,舟山,316004 2. 江南大学食品学院,无锡,214036 3. 江南大学生物工程学院,无锡,214036 |
| |
摘 要: | 以自筛曲霉CJ2 2 -3为出发株 ,经紫外线和6 0 Co诱变处理 ,获得 1株壳聚糖酶高产菌株CJ2 2 -3 2 6,经正交实验初步优化了其液态产酶培养基 :壳聚糖 1 5 % ,麸皮 2 % ,(NH4 ) 2 SO4 0 2 % ,KH2 PO4 0 2 % ,MgSO4 0 0 5 % ,Tween -80 0 0 5 % ,pH5 5。优化的发酵条件为 :装液量 75mL/ 2 5 0mL三角瓶 ,摇床转速 1 5 0r/min ,发酵温度为 3 0℃ ,发酵时间为 96h。在此条件下 ,CJ2 2 -3 2 6产酶活力为3 0 6U/mL。
|
关 键 词: | 曲霉 选育 发酵条件 壳聚糖酶 |
修稿时间: | 2003年9月24日 |
Breeding of Chitosanase Producing Strain and Study on Its Fermentation Condition |
| |
Abstract: | Aspergillus CJ 22-326, with high yield of chitosanase, was a mutant strain of Aspergillus CJ 22-3 treated by UV and γ-ray. The compositions of culture medium in flask determined by orthogonal experiment were 1.5% colloidal chitosan, 2.0% bran, 0.2% KH 2PO 4, 0.2% (NH 4) 2SO 4, 0.05% MgSO 4, 0.05% Tween-80, pH5.5. The optimal culture conditions for chitosanase production were: medium volume 75 mL in 250 mL flask, rotation speed 150r/min, temperature 30℃ and culture time 96h. Under these conditions, the chitosanase activity produced by Aspergillus CJ 22-326 reached 3.06 U/mL. |
| |
Keywords: | Aspergillus chitosanase breeding fermentation condition chitosanase |
本文献已被 CNKI 维普 万方数据 等数据库收录! |
|