高效液相色谱-二极管阵列检测法测定黄酒中β-苯乙醇的含量

研究了以液相色谱 二极管阵列检测法分离测定黄酒中 β 苯乙醇的方法。采用Lichro spherR○1 0 0ODS C18柱 ,以 0 0 2mol/LKH2 PO4 溶液 (pH4 5 ) 甲醇 (体积比为 5 5∶45 )作流动相 ,流速 1 0mL/min ,用二极管阵列检测器于 2 1 0nm下进行检测 ,并以保留时间和光谱图相似性系数进行定性。方法线性范围为 5 0～ 40 0 μg/mL(r =0 9999) ,检测限 0 2 μg/mL ,相对标准偏差为 0 74% (n =7) ,回收率为 99 1 % ± 0 63 % (n =5 )。测定结果与气相色谱法 (GC法 )无显著性差异 (P <0 0 5 )。

Determination of β-Phenylethanol in Chinese Rice Wine by Reversed Phase High Performance Liquid Chromatography-Diode Array Detector

A method for determines of β-phenylethanol in Chinese rice wine by high performance liquid chromatography-diode array detector(DAD)was studied. In this method, a Lichrospher DR○ 100 ODS-C 18 column was used with 0.02mol/L potassium phosphate monobasic buffer (pH4.5) and methanol (55∶45, V/V) as mobile phase. The flow rate was 1.0*#mL/min. A diode array detector was used and the detection wavelength was 210*#nm. The calibration curve was linear over a range from 5.0*#μg/mL to 40.0*#μg/mL with a correlation coefficient of 0.9999. Qualitative analysis was performed with retention time and DAD spectrum similarity index value. The detection limit was 0.2*#μg/mL, the relative standard deviation was 0.74%(n=7), and the recoveries were 99.1%±0.63%(n=5). The determination results show no significant difference between this method and gas chromatography(P<0.05).