| 用于PCR检测的乳品中金黄色葡萄球菌DNA提取方法比较研究 |
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| 引用本文： | 杨洋,张伟,袁耀武,何俊萍,马晓燕,丁鑫,王呸玉.用于PCR检测的乳品中金黄色葡萄球菌DNA提取方法比较研究[J].食品与发酵工业,2005,31(9):91-95. |
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| 作者姓名： | 杨洋 张伟 袁耀武 何俊萍 马晓燕 丁鑫 王呸玉 |
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| 作者单位： | 1. 河北农业大学食品科技学院,保定,071001
2. 廊坊食品工程学院,廊坊,065000 |
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| 摘 要： | 比较了乳品中金黄色葡萄球菌DNA的6种提取方法的效果,确定了一种可有效从乳品中提取金黄色葡萄球菌DNA的方法。该方法无需对样品进行增菌,可直接从乳品中提取金黄色葡萄球菌DNA,作为模板进行PCR检测,检测效果良好,对UHT全脂乳、脱脂乳和奶酪的最低检出限分别为10、10CFU/mL和55CFU/g。
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| 关 键 词： | 乳品 金黄色葡萄球菌 DNA提取 PCR |
| 收稿时间： | 03 30 2005 12:00AM |
| 修稿时间： | 06 3 2005 12:00AM |
Comparison of the Methods of Extracting DNA from Staphylococcus aureus in Dairy Products |
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| Yang Yang,Zhang Wei,Yuan Yaowu,He Junping,Ma Xiaoyan,Ding Xin,Wang Peiyu.Comparison of the Methods of Extracting DNA from Staphylococcus aureus in Dairy Products[J].Food and Fermentation Industries,2005,31(9):91-95. |
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| Authors: | Yang Yang Zhang Wei Yuan Yaowu He Junping Ma Xiaoyan Ding Xin Wang Peiyu |
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| Affiliation: | 1.College of Food Science and Technology, Agricultural University of Hebei, Baoding, 071001, China |
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| Abstract: | The effects of 6 methods of extracting DNA from Staphylococcus aureus in dairy products were compared. An efficient extraction procedure was confirmed for extraction of Staphylococcus aureus DNA from dairy products. Staphylococcus aureus DNA was directly extracted from dairy products without enrichment. The extracted DNA was suitable for PCR detection. The sensitivity of the uniplex PCR is 10CFU/mL of UHT whole milk,skim milk and 55CFU/g cheese. |
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| Keywords: | dairy products Staphylococcus aureus DNA extraction PCR |
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