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| 直接皂化比色法测定食品中胆固醇的研究 | |
| 引用本文： | 张佳程,骆承庠,黄赤男.直接皂化比色法测定食品中胆固醇的研究[J].食品与发酵工业,2000,26(3):35-38. |
| 作者姓名： | 张佳程 骆承庠 黄赤男 |
| 作者单位： | 1. 河北农业大学食品系,保定,071001 2. 东北农业大学食品学院,哈尔滨,150030 3. 大庆市农业工作办公室,大庆,163311 |
| 摘 要： | 研究利用氢氧化钾 甲醇溶液在 80℃水浴中对食品样品 (蛋黄粉和奶粉 )直接皂化30min ,并在皂化混合物中使用抗氧化剂 ,然后用正己烷萃取并离心 ,取出一定量正己烷层挥发干后 ,用FeSO4 冰乙酸 浓硫酸作显色剂在 4 90nm下测定胆固醇含量。该方法具有良好的线性 ,与气相色谱法测定的结果差异不显著。这为今后在生产和科研中快速准确地测定食品中胆固醇含量提供了有效手段。 |
| 关 键 词： | 胆固醇 比色法 蛋黄 奶粉 |
| 修稿时间： | 1999-05-23 |
Cholesterol Analysis in Foods by Direct Saponification-colorimetric Method |
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| Zhang Jiacheng,Luo Chengxiang,Huang Chinan.Cholesterol Analysis in Foods by Direct Saponification-colorimetric Method[J].Food and Fermentation Industries,2000,26(3):35-38. | |
| Authors: | Zhang Jiacheng Luo Chengxiang Huang Chinan |
| Affiliation: | Zhang Jiacheng ;(Department of Food Science, Agricultural University of Hebei, Baoding, 071001);Luo Chengxiang ;(College of Food Science, Northeast Agricultural University, Herbin, 150030);Huang Chinan ;(Administrative Office of Agriculture, Daqing City, Daqing, 163311) |
| Abstract: | The method for analyzing cholesterol involved direct saponification of food samples (egg yolk powder and milk powder) at 80℃/30min by saturated methanolic KOH with an antioxidant, extraction with hexane and centrifugation, removal of hexane in an aliquot of extract, color developing by FeSO 4 glacial acetic acid H 2SO 4 and determination at 490nm. Cholesterol values obtained by this method and GC procedure were not significantly different (P>0.05). The method could be applied in research and production as a rapid and accurate method for analyzing cholesterol in foods. |
| Keywords: | cholesterol colorimetric method egg yolk milk powder |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |