鸡蛋卵转铁蛋白螯合铁及其对大鼠贫血模型的影响

将从鸡蛋中分离纯化的卵转铁蛋白与FeCl3结合,研究其在不同铁离子浓度、pH值、NaHCO3和温度条件下与铁离子的结合能力。通过色度仪、红外光谱仪、粒径分析仪、扫描电镜等仪器研究冷冻和喷雾干燥对螯合铁卵转铁蛋白理化性质的影响。在0.4 mmol/L FeCl3,120 mmol/L NaHCO3,pH 8.0,温度低于60℃时,卵转铁蛋白铁结合能力为91.5%,结合的铁含量为1.28μg/g。冷冻和喷雾干燥对螯合铁卵转铁蛋白的色度、粒径分布、完全溶解时间存在极显著的差异,而在水分活度和化学键结构方面无明显差异。建立缺铁大鼠模型,考察30 d内卵转铁蛋白对大鼠贫血的改善作用,结果表明:冷冻和喷雾干燥处理的螯合铁卵转铁蛋白对贫血大鼠体重和脏器指数升高有显著作用;对血常规中的红细胞计数、血红蛋白浓度、血细胞压积、红细胞平均体积均有正向促进作用(P<0.01),能够显著增加大鼠血液中血清铁含量,提高血清铁蛋白含量,降低血清总铁结合力(P<0.05),部分指标优于对照组硫酸亚铁。卵转铁蛋白可作为一种生物补铁剂显著改善大鼠的缺铁性贫血状况。

The Iron-binding of Ovotransferrin and Its Effect on Iron-deficiency Anemia in Rats

The ovotransferrin was extracted and purified from chicken egg. The iron binding capacity of ovotransferrin was studied with different concentration of Fe3+ or NaHCO3, pH value and temperature. The effects of freeze-drying and spray-drying on the physical and chemical properties of ovotransferrin-Fe (Ⅲ) were investigated by colorimeter, infrared spectrometer, particle size analyzer and scanning electron microscope, etc. The iron binding capacity of ovotransferrin was good(capacity 91.5%, iron content 1.28 μg/g). In the condition of 0.4 mmol/L FeCl3 pH 8.0;120 mmol/L NaHCO3 and temperature below 60 ℃. There were significant differences in the chromaticity, particle size distribution or complete dissolution time of freeze-drying ovotransferrin-Fe(Ⅲ) and spray-drying ovotransferrin-Fe(Ⅲ), but there was no significant difference in water activity or chemical bon. After 30 days of animal experiments, freeze-drying and spray-drying group had significant effects on the increase in body weight and organ index in the anemic rats, as well as the red blood cell count, hemoglobin concentration, hematocrit, erythrocyte(P <0.01). Both groups can significantly increase serum iron content, serum ferritin content, and decrease total iron binding capacity(P<0.05). Some indexes of ovotransferrin group were even better than ferrous sulfate control group. Therefore, ovotransferrin can be used as a biological iron supplement,which can significantly improve the condition of rat iron deficiency anemia.