外源硒处理的花生芽蛋白中五种硒形态分析

为了探究花生发芽后的富硒规律，本研究用不同浓度Na2SeO3浸泡花生子粒发芽5d后，分析外源硒对花生发芽情况的影响，并分别通过电感耦合等离子体质谱法（Inductively Coupled Plasma-Mass Spectrometry，ICP-MS）和高效液相色谱-电感耦合等离子体质谱联用（High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry，HPLC-ICP-MS）分析花生芽不同部位（子叶、胚轴、胚根）的总硒含量和花生芽蛋白中5种硒形态（硒代胱氨酸SeCys2、硒代半胱氨酸MeSeCys、亚硒酸根SeIV、硒代蛋氨酸SeMet、硒酸根SeVI）的组成特征及变化规律。结果表明，随着Na2SeO3浸泡浓度的升高，花生芽长、芽粗和发芽率均呈降低的趋势；高浓度的外源硒抑制了花生发芽过程中硒元素由子叶向胚轴和胚根的转移；未经硒浸泡的花生发芽后，三个部位均检测到三种硒形态，其中SeCys2占比最高，随着硒浓度升高，各部位硒形态种类增加，无机硒（SeIV和SeVI）占比逐渐升高，SeCys2和MeSeCys占比逐渐降低，SeMet占比没有规律性变化，各部位有机硒总量占比降低，无机硒向有机硒转化率收到抑制。

Selenium speciation analysis of peanut sprouts protein treated with exogenous selenium

In order to explore the law of selenium enrichment in peanut sprout, we studied the effects of exogenous selenium on the peanut seeds germination after being soaked in Na2SeO3 for 5 days. The selenium contents and species (SeCys2, MeSeCys, SeIV, SeMet, SeVI) in different parts of peanut sprout (cotyledons, hypocotyls and radicles) were analyzed by ICP-MS and HPLC-ICP-MS respectively. The results showed that with the increase of Na2SeO3 concentration, the peanut sprout length, diameter and germination rate all showed a decreasing trend; High concentration of exogenous selenium inhibited the transfer of selenium from cotyledon to hypocotyl and radicle during peanut germination; Three kinds of selenium species were detected in all parts of peanut sprout without selenium soaking, and the proportion of SeCys2 was highest. With the increase of exogenous selenium concentration, the selenium speciation and the proportion of inorganic selenium (SeIV and SeVI) increased, The proportion of SeCys2 and MeSeCys decreased gradually, but that of SeMet did not change regularly, total organic selenium in each part was reduced, and the conversion of Inorganic selenium to organic selenium was inhibited.