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| 浓香型白酒曲药中细菌组成及系统学分析 | |
| 引用本文： | 胡佳,邓斌,张文学,张宿义,罗俊成,胡承.浓香型白酒曲药中细菌组成及系统学分析[J].酿酒科技,2007(5):17-19. |
| 作者姓名： | 胡佳 邓斌 张文学 张宿义 罗俊成 胡承 |
| 作者单位： | 1. 四川大学生物资源与生态环境教育部重点实验室,四川,成都,610064 2. 重庆市江津计量质量检测中心,重庆,綦江,401420 3. 四川泸州老窖股份有限公司,四川,泸州,646000 |
| 基金项目： | 四川省精品课程建设资助项目 |
| 摘 要： | 利用免培养法(Culture independent approach)提取浓香型白酒曲药中细菌的总DNA,在分子水平上运用PCR扩增技术和16S rDNA序列同源性分析等方法测定曲药中细菌的16S rDNA基因近全序列,并建立系统发育树图.结果表明,组成该曲药中的细菌分属于Delftia、Dysgonomonas、Bacteroidetes,proteobacterium、Nocardiopsis、Pseudomonas和Arthrobacter几大类群(phylum),表现出高度的细菌多样性. |
| 关 键 词： | 微生物 大曲 16S rDNA序列 PCR扩增 免培养法 细菌 浓香型 酒曲 细菌组成 系统学分析 Composition Bacterial Analysis 细菌多样性 高度 表现 类群 Arthrobacter Pseudomonas 分属 结果 树图 系统发育 全序列 基因 测定 |
| 文章编号： | 1001-9286（2007）05-0017-03 |
| 修稿时间： | 2007-03-09 |
Phylogenetic Analysis of Bacterial Composition In Luzhou-flavor Liquor Quyao |
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| HU Jia,DENG Bin,ZHANG Wen-xue,ZHANG Su-yi,LUO Jun-cheng,HU Cheng.Phylogenetic Analysis of Bacterial Composition In Luzhou-flavor Liquor Quyao[J].Liquor-making Science & Technology,2007(5):17-19. | |
| Authors: | HU Jia DENG Bin ZHANG Wen-xue ZHANG Su-yi LUO Jun-cheng HU Cheng |
| Abstract: | In order to know the bacterial composition in Luzhou-flavor liquor Quyao, the techniques including culture independent approach, PCR technique and 16S rDNA sequence homology analysis etc. were used to measure 16S rDNA gene total order of bacteria in Quyao and the phylogenetic tree was further established. According to the sequences alignment and through the analysis of its comparability, the bacteria of Luzhou-flavor Quyao were consisted of Delfiia, Dysgonomonas, Bacteroidetes, proteobacterium, Nocardiopsis, Pseudomona and A rthrobacter, which indicated their bacterial diversity. |
| Keywords: | microbe Luzhou-flavor liquor quyao 16S rDNA sequence PCR Culture independent approach bacteria |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |