| 重组酶聚合酶快速扩增法测定瓶(桶)装水中铜绿假单胞菌 |
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| 引用本文： | 刘 辉,张 娟,张 燕,赵丹霞,吴胜泽.重组酶聚合酶快速扩增法测定瓶(桶)装水中铜绿假单胞菌[J].食品安全质量检测技术,2020,11(19):6915-6920. |
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| 作者姓名： | 刘 辉 张 娟 张 燕 赵丹霞 吴胜泽 |
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| 作者单位： | 广东产品质量监督检验研究院,广东产品质量监督检验研究院,广东产品质量监督检验研究院,广东产品质量监督检验研究院,广东产品质量监督检验研究院 |
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| 基金项目： | 广州市科技计划项目(201804010244)；广州市科技计划项目(201904010102)；农业部农产品贮藏保鲜质量安全风险评估实验室(广州)暨广东省食品质量安全重点实验室开放课题(2019KF007) |
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| 摘 要： | 目的建立重组酶聚合酶扩增法(recombinasepolymeraseamplification,RPA)检测瓶(桶)装水中铜绿假单胞菌(Pseudomonasaeruginosa)的分析方法。方法通过基因组DNA提取、RPA扩增反应方法对样品进行检测。结果该方法能够在20 min内特异地检测出铜绿假单胞菌,方法检出限为10 pg/μL铜绿假单胞菌基因组模板,对人工污染的水样最低检出限为36 CFU/mL,且重复性良好。结论本研究建立的RPA检测方法能特异、准确、高效地检测出铜绿假单胞菌,而且操作简单、耗时短,为瓶(桶)装水中铜绿假单胞菌的快速诊断提供技术参考。
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| 关 键 词： | 瓶(桶)装水 铜绿假单胞菌 重组酶聚合酶扩增 |
| 收稿时间： | 2020/6/29 0:00:00 |
| 修稿时间： | 2020/9/27 0:00:00 |
Establishment of a recombinase polymerase amplification technology to detect Pseudomonas aeruginosa in bottled (or barreled) water |
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| LIU Hui,ZHANG Juan,ZHANG Yan,ZHAO Dan-Xi,WU Sheng-Ze.Establishment of a recombinase polymerase amplification technology to detect Pseudomonas aeruginosa in bottled (or barreled) water[J].Food Safety and Quality Detection Technology,2020,11(19):6915-6920. |
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| Authors: | LIU Hui ZHANG Juan ZHANG Yan ZHAO Dan-Xi WU Sheng-Ze |
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| Affiliation: | Sericultural Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing;China;Guangzhou Lehel Clinical Nutrition CoLtd;China;Guangdong Testing Institute for Product Quality Supervision;China,Sericultural Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing;China;Guangzhou Lehel Clinical Nutrition CoLtd;China;Guangdong Testing Institute for Product Quality Supervision;China,Sericultural Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing;China;Guangzhou Lehel Clinical Nutrition CoLtd;China;Guangdong Testing Institute for Product Quality Supervision;China,Sericultural Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing;China;Guangzhou Lehel Clinical Nutrition CoLtd;China;Guangdong Testing Institute for Product Quality Supervision;China,Sericultural Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing;China;Guangzhou Lehel Clinical Nutrition CoLtd;China;Guangdong Testing Institute for Product Quality Supervision;China |
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| Abstract: | Objective Pseudomonas aeruginosa is routinely detected in bottled (or barreled) water. The detection rate is high in daily monitoring.In order to reduce the economic loss of enterprises, it is urgent to establish a rapid and accurate detection method. Methods This study established a recombinase polymerase amplification (RPA) technology for detecting Pseudomonas aeruginosa. Results The results showed that the method could detect Pseudomonas aeruginosawithin 20 minutes and the method detection limit was 10 pg/template.The minimum detection limit of artificial pollution water sample was3.6×102 CFU/mL. The method repeatability was good. Conclusion The established RPA detection method can detect Pseudomonas aeruginosa specifically, accurately and efficiently, and it is simple to operate and less time-consuming.It will provide a technical reference for the rapid diagnosis of Pseudomonas aeruginosa in bottled (or barreled) water. |
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| Keywords: | bottled (or barreled) water Pseudomonas aeruginosa recombinase polymerase amplification |
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