多内标校正-电感耦合等离子体质谱法测定大豆中25种元素的含量

目的 建立多内标校正-电感耦合等离子体质谱法同时测定大豆中25种元素含量的方法。方法 通过对比微波消解法和直接提取法等不同前处理方式的优劣, 优化确定最佳前处理方式; 评估基体效应对测定结果的影响, 并选择多个合适的内标对基体效应进行校正后, 各元素含量由电感耦合等离子体质谱法进行检测。结果 直接提取法无法将元素完全地从大豆中提取出来, 微波消解法具有更好的提取效率; 各元素在大豆基质中有较明显的基体效应, 测定过程中必须使用多内标进行校正。各元素加标回收率为78.27%~103.2%, 重复性为0.72%~5.62%。结论 本方法定量准确、灵敏, 适用于大豆中多种元素含量的测定。

Determination of 25 kinds of elements in soybean by inductively coupled plasma mass spectrometry with multiple internal standard correction

Objective To establish a quantitative method for simultaneous determination of 25 elements in soybean by inductively coupled plasma mass spectrometry with multiple internal standard correction. Methods By comparing the advantages and disadvantages of different pre-processing methods such as microwave digestion method and direct extraction method, the best pre-processing method was optimized and determined. The influence of matrix effect on the determination results was evaluated, a number of appropriate internal standards were selected to correct the matrix effect, and the content of each element was detected by inductively coupled plasma mass spectrometry. Results The direct extraction method could not completely extract the elements from soybean, and microwave digestion method had better extraction efficiency. Each element had obvious matrix effect in soybean matrix, and multiple internal standards must be used to correct the determination process. The recovery rates of each element were 78.27%?103.2%, and the repeatabilities were 0.72%?5.62%. Conclusion This method is accurate, sensitive and suitable for the determination of various elements in soybean.