| 基于特征肽段结合超高效液相色谱-串联质谱法的乳制品真实性鉴别方法研究 |
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| 引用本文： | 陈荣桥,冼燕萍,梁 明,胡均鹏,吴玉銮,奚晓翔,戴 航,侯向昶.基于特征肽段结合超高效液相色谱-串联质谱法的乳制品真实性鉴别方法研究[J].食品安全质量检测技术,2023,14(13):258-265. |
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| 作者姓名： | 陈荣桥 冼燕萍 梁 明 胡均鹏 吴玉銮 奚晓翔 戴 航 侯向昶 |
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| 作者单位： | 广州质量监督检测研究院,广州质量监督检测研究院,广州质量监督检测研究院,广州质量监督检测研究院,广州质量监督检测研究院,广州质量监督检测研究院,广州质量监督检测研究院,广州质量监督检测研究院 |
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| 基金项目： | 广州市科技计划项目(2023B04J0407), 国家重点研发计划项目(2019YFC1606400) |
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| 摘 要： | 目的 基于超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)建立乳制品中乳铁蛋白、乳桥蛋白、奶牛A1 β-酪蛋白和A2 β-酪蛋白、水牛A2 β-酪蛋白的检测方法, 开展市售乳制品中功能性蛋白质检测和乳制品真实性鉴评分析。方法 乳蛋白经酶解后, 应用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法(ultra performance liquid chromatography-triple quadrupole-Orbitrap high resolution mass spectrometry, UPLC-Q/Orbitrap HRMS)和UPLC-MS/MS筛选获得可用于定性定量分析的特征肽段, 基于UPLC-MS/MS建立同时检测LF、LPN、奶牛A1 β-CN、奶牛A2 β-CN和水牛A2 β-CN的方法并应用于乳制品检测。结果 5种待测蛋白在相应的浓度范围内线性关系良好(r2>0.99), 加标回收率在82.1%~105.5%之间, 相对标准偏差均小于5%, 78份乳制品的检测结果表明同类样品LF含量差异较大，不同类液体乳中LPN均值差异不大。结论 本方法性能良好, 适用于乳制品中乳桥蛋白等功能性蛋白的同时检测, 也可以实现乳制品中乳蛋白物种来源的鉴别。
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| 关 键 词： | 功性能乳蛋白 靶向蛋白质组学 超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法 超高效液相色谱-串联质谱法 鉴别 |
| 收稿时间： | 2023/4/14 0:00:00 |
| 修稿时间： | 2023/6/19 0:00:00 |
Study on the authenticity identification of dairy products based on characteristic peptides combined with ultra performance liquid chromatography-tandem mass spectrometry |
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| CHEN Rong-Qiao,XIAN Yan-Ping,LIANG Ming,HU Jun-Peng,WU Yu-Luan,XI Xiao-Xiang,DAI Hang,HOU Xiang-Chang.Study on the authenticity identification of dairy products based on characteristic peptides combined with ultra performance liquid chromatography-tandem mass spectrometry[J].Food Safety and Quality Detection Technology,2023,14(13):258-265. |
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| Authors: | CHEN Rong-Qiao XIAN Yan-Ping LIANG Ming HU Jun-Peng WU Yu-Luan XI Xiao-Xiang DAI Hang HOU Xiang-Chang |
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| Affiliation: | Guang zhou Quality Supervision and Testing Institute,Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety,Guangzhou City Key Laboratory of Detection Technology for Food Safety,Guang zhou Quality Supervision and Testing Institute,Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety,Guangzhou City Key Laboratory of Detection Technology for Food Safety,Guang zhou Quality Supervision and Testing Institute,Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety,Guangzhou City Key Laboratory of Detection Technology for Food Safety,Guang zhou Quality Supervision and Testing Institute,Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety,Guangzhou City Key Laboratory of Detection Technology for Food Safety,Guang zhou Quality Supervision and Testing Institute,Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety,Guangzhou City Key Laboratory of Detection Technology for Food Safety,Guang zhou Quality Supervision and Testing Institute,Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety,Guangzhou City Key Laboratory of Detection Technology for Food Safety,Guang zhou Quality Supervision and Testing Institute,Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety,Guangzhou City Key Laboratory of Detection Technology for Food Safety,Guang zhou Quality Supervision and Testing Institute,Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety,Guangzhou City Key Laboratory of Detection Technology for Food Safety |
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| Abstract: | |
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| Keywords: | functional proteins targeted proteomics ultra performance liquid chromatography-triple quadrupole- Orbitrap high resolution mass spectrometry ultra performance liquid chromatography-tandem mass spectrometry authenticity identification |
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