调味品中合成着色剂和糖精钠的测定

目的 建立了一种同时测定调味品中合成着色剂（诱惑红、胭脂红、日落黄）和糖精钠的高效液相色谱（HPLC）分析方法.方法 采用C18色谱柱,以甲醇和0.02 mol/L乙酸铵溶液为流动相,梯度洗脱,光电二极管阵列（PDA）检测器检测,采用外标法定量分析.结果 4种成分的线性范围为0.5～20.0 μg/mL （r＞0.999）,回收率为94.3％～101.6％,RSD为0.42％～2.44％（n=6）.结论 此法准确、灵敏,前处理简单,适用于调味品中诱惑红、胭脂红、日落黄和糖精钠的定性、定量分析.

Determination of Synthetic Colourants and Crystallose in Condiments

Objective To establish a HPLC method for the simultaneous determination of synthetic colourants（allura red,ponceau 4R,sunset yellow） and crystallose in condiments.Methods The samples were separated on a C 18 column by a gradient elution with methanol and 0.02 mol/L ammonium acetate as mobile phase.The target compounds were confirmed by photo-diode array detection and quantified by the external standard method.Results The standard curves of the target compounds were linear within the range of 0.5-20 μg/mL,with recovey of 94.3 ％-101.6 ％（r＞ 0.999） and RSD of 0.42 ％-2.44 ％（n=6）.Conclusion The method with simple pre-processing is sensitive and accurate,and can be used to determine allura red,ponceau 4R,sunset yellow and crystallose in condiments.