大孔树脂纯化美藤果壳酚类物质的研究

以美藤果壳为原料,用70%乙醇提取得到美藤果壳酚类粗提物,研究大孔树脂纯化美藤果壳酚类物质的工艺条件,对AB-8、HPD300、NKA-9、D101、S-8、DM130 6种型号大孔树脂进行了筛选。结果表明:NKA-9型大孔树脂为纯化美藤果壳酚类物质最佳树脂;NKA-9型大孔树脂纯化美藤果壳酚类物质的最佳工艺条件为上样液质量浓度10 mg/m L、上样速度2.0 m L/min、以70%的乙醇溶液洗脱、洗脱速度1.0 m L/min、洗脱剂体积2.5 BV。在最佳工艺条件下纯化后的美藤果壳酚类物质的总酚含量为73.8%,对比于粗提物中的总酚含量33.2%,提高了1.2倍。     

美藤果壳多酚物质的提纯及其抗氧化性

以美藤果壳为主要原料,用乙醇提取法提取美藤果壳多酚,考察乙醇浓度、料液比、提取温度、提取时间对美藤果壳多酚得率的影响.在单因素试验的基础上,进行正交试验优化提取工艺参数后,通过大孔树脂纯化粗提物,采用高效液相色谱法(high performance liquid chromatography,HPLC)测定其中主要多酚...     

美藤果壳提取物降血压功效的研究

以血管紧张素转化酶(angiotensin converting enzyme,ACE)抑制率为指标,筛选美藤果壳提取物降血压成分的最佳提取溶剂和壳层,并结合自发性高血压大鼠(spontaneously hypertensive rat,SHR)模型,初步评价美藤果壳提取物的降血压功效。体外和体内实验结果表明:以水作为提取溶剂,美藤果壳提取物(sacha inchi shell extract,SISE)的ACE活性抑制率最高,其活性显著高于菊花、银杏提取物等其他天然ACE抑制剂,且高剂量(400 mg/kg·BW)的SISE显著降低了SHR大鼠的血压,给药1 h后SHR大鼠收缩压由166 mmHg显著降低至135 mmHg。结果初步证实了SISE具备降血压功效。急性经口毒性试验表明SISE属于食品级无毒。通过对SISE有效降压成分进行分离,表明其有效降压成分主要集中在正丁醇萃取物中,其萃取物经硅胶柱,氯仿/甲醇梯度洗脱,氯仿/甲醇(7∶3)组分的ACE抑制效果最佳。该研究为进一步开发美藤果壳为天然降血压药物夯实基础。     

花生芽中酚类物质的大孔树脂纯化工艺研究

以花生芽为原料,采用大孔树脂纯化其中酚类物质。通过对比7种型号大孔树脂对花生芽多酚的吸附和解吸效果,筛选出AB-8为最佳树脂类型,并对其静态吸附-解吸条件和动态吸附-解吸条件进行优化。结果表明,AB-8大孔树脂对花生芽中酚类物质的最佳静态吸附-解吸条件为:吸附时间6 h、样液pH 3、样液质量浓度2.0 mg/mL、解吸时间6 h、乙醇浓度60%、解吸液pH 3。最佳动态吸附-解吸条件为上样浓度1.0 mg/mL、上样流速1.5 mL/min,乙醇浓度60%、洗脱流速1.5 mL/min。     

麦类麸皮中酚类物质提取纯化方法的研究进展

麦类麸皮中含有多种酚类物质,主要有酚酸、黄酮、木酚素等。研究表明,这些酚类物质具有良好的抗氧化活性,在食品和医药领域具有广泛的应用前景。该文综述了麦类麸皮中酚类物质提取的研究进展和现状,以期为将来相关产品以及研究提供参考和理论基础。     

宁海白枇杷花中酚类物质鉴定及含量测定

运用HPLC-PAD-MS技术对宁海白枇杷花中酚类化合物进行定性及定量分析,共检测到6种主要酚类化合物,4种被鉴定。其中,异槲皮苷含量最高,达到1.536mg/g;绿原酸0.5550mg/g;槲皮苷0.5836mg/g;槲皮素含量较低,0.2294mg/g。通过回收率试验、系统稳定性试验及日间精密度试验对该方法进行验证,结果显示各物质的回收率在90.3%～101.2%之间,方法准确性好;各标品峰高及峰面积的变异系数均小于1%(n=3),系统稳定性较好;各标品保留时间的变异系数均小于1%,日间精密度良好。本分析方法具有快速、灵敏、准确的优点,适用于枇杷花中酚类物质的定性及定量分析。     

辅酶Q10的纯化和鉴定方法研究

目前国内外辅酶Q_(10)的鉴定方法较多,主要为物理和化学方法相结合:测定其熔点是否为49℃;用Craven氏颜色实验法,在碱性条件下氰基乙酸乙酯可取代辅酶Q_(10)的甲氧基生成很强的蓝色化合物;用Wiss-Brubacher法,使之与醋酸酐-硫酸-冰醋酸形成蓝色;另外还有薄层层析法、质谱、红外及紫外吸收光谱法,质谱可以更精确地对辅酶Q_(10)作出鉴定,红外则是根据官能基团的特征吸收来鉴定,紫外吸收光谱是利用乙醇作为溶剂,在220-320 nm内测定氧化型光谱,然后加入硼氢化钠溶液,在相同的波长下测定还原型光谱,其氧化     

多糖与酚类物质的相互作用研究进展

多糖和酚类两种生物大分子相互作用主要通过结构的改变,进而影响生物体的功能活性,并起到协同增效的作用,在生物医药、保健食品、化妆品等方面具有重要的应用价值。本文从协同作用效果和结构分析及实际应用中对多糖和酚类物质的相互作用进行综述。      

琯溪蜜柚果汁树脂吸附脱苦前后酚类物质含量的测定

利用高效液相色谱法测定了琯溪蜜柚果汁酚类成分的含量,并研究了吸附脱苦对琯溪蜜柚果汁中酚类物质含量的影响。结果表明,琯溪蜜柚果汁中含有绿原酸、咖啡酸、香豆酸、阿魏酸、芸香苷和柚皮苷等六种酚类物质;用高效液相色谱法分析琯溪蜜柚果汁中酚类物质,它们的加标回收率均在85.0%¹03.6%之间,相对标准偏差（RSD）均在0.48%².9%之间。经XAD-16树脂吸附脱苦后,柚汁中的香豆酸、阿魏酸、芸香苷等三种酚类成分已检测不到,而绿原酸、柚皮苷和咖啡酸的损失率分别为97.2%、93.5%、87.9%,说明树脂吸附脱苦导致琯溪蜜柚汁中绿原酸、咖啡酸、香豆酸、阿魏酸、芸香苷和柚皮苷等六种酚类物质含量严重降低。      

琯溪蜜柚果汁树脂吸附脱苦前后酚类物质含量的测定

利用高效液相色谱法测定了琯溪蜜柚果汁酚类成分的含量,并研究了吸附脱苦对琯溪蜜柚果汁中酚类物质含量的影响。结果表明,琯溪蜜柚果汁中含有绿原酸、咖啡酸、香豆酸、阿魏酸、芸香苷和柚皮苷等六种酚类物质;用高效液相色谱法分析琯溪蜜柚果汁中酚类物质,它们的加标回收率均在85.0%~103.6%之间,相对标准偏差(RSD)均在0.48%~2.9%之间。经XAD-16树脂吸附脱苦后,柚汁中的香豆酸、阿魏酸、芸香苷等三种酚类成分已检测不到,而绿原酸、柚皮苷和咖啡酸的损失率分别为97.2%、93.5%、87.9%,说明树脂吸附脱苦导致琯溪蜜柚汁中绿原酸、咖啡酸、香豆酸、阿魏酸、芸香苷和柚皮苷等六种酚类物质含量严重降低。     

Preparative separation and purification of phenolic compounds from Canarium album L. by macroporous resins

BACKGROUND: Canarium album L. (also called Chinese olive) is a traditional medicine material in China, and phenolic compounds from C. album possess great pharmacological activities. To obtain high‐purity phenolics from C. album, a crude extract of C. album phenolics was prepared by ethanol extraction. The use of macroporous resins for further separation and purification of phenolics in the extract was studied. RESULTS: Through static adsorption and desorption tests, AB‐8 resin was chosen for the separation of phenolics because of its higher adsorption capacity and desorption ratio than other resins. Then, dynamic adsorption and desorption experiments were carried out on an AB‐8 resin packed column to obtain optimal separation parameters. The highest adsorption capacity of AB‐8 was achieved when variables including initial concentration (C₀), feed flow rate and feed volume were 10 mg mL^?1, 2 mL min^?1 and 9 bed volumes (BV), respectively, and saturated resin was first washed with 5 BV of water to remove impurities, then a purified product containing more than 85% of C. album phenolics was obtained by desorbing the resin with 2.5 BV of 70% (v/v) aqueous ethanol at flow rate of 1 mL min^?1, and the recovery of phenolics was up to 75%. In addition, five phenolic compounds in the product were identified as gallic acid, ellagic acid, corilagin, hyperin and kaempferol‐3‐glucopyranoside by UV and LC–ESI–MS analysis. CONCLUSION: The results in this study could provide scientific references for the large‐scale production of phenolics from C. album. Copyright © 2007 Society of Chemical Industry     

薄层色谱法鉴别大黄饮片真伪

目的建立一种以土大黄苷为检测指标,定性鉴别中药大黄饮片真伪的薄层色谱(TLC)方法。方法以甲醇为提取溶剂,用超声波提取的方法制备供试品溶液,以硅胶G板为固定相,苯-甲酸乙酯-甲酸-甲醇(30:10:0.1:15)为展开剂展开,紫外光(365 nm)下检视。结果华北大黄和河套大黄两种伪品大黄饮片的薄层色谱中检出土大黄苷,正品大黄饮片中未检出;对市售的10批大黄饮片进行TLC鉴别,有50%检出土大黄苷成分。结论该方法操作简便、结果准确、专属性强,可用于大黄饮片的真伪鉴别,有一定的应用价值。     

超声波辅助提取野桂花蜜中多酚的工艺优化

为确定野桂花蜜中多酚化合物提取的最佳工艺参数,以野桂花蜜为主要原料,采用响应面优化野桂花蜜中多酚化合物的超声波辅助提取工艺。在单因素试验的基础上,以超声波时间、超声波温度、料液比、溶剂浓度为自变量,以多酚化合物得率为响应值,进行试验设计,研究自变量与响应值之间的关系。结果表明:优化得出的最佳工艺条件为溶剂浓度62%,料液比1︰10(m︰V),超声波时间21 min,超声波温度54℃。该工艺条件下多酚得率为68.27mg/10g。     

Purification and identification of rice bran (Oryza sativa L.) phenolic compounds with in-vitro antioxidant and antidiabetic activity using macroporous resins

This study was designed to establish an efficient purification method for phenolic compounds from rice bran and to acquire insightful knowledge of purification impact on its chemical constituents, antioxidant and antidiabetic activity. Among the eight macroporous resins investigated, HPD-300 resin was verified to bosses superior adsorption and desorption qualities for the extract. Purification parameters were optimised. Eighteen phenolic compounds were identified using HPLC-PDA, the contents of ferulic acid and syringaldehyde were significantly (P < 0.05) higher than the other constituents. However, phenolic compounds concentrations varied significantly (P < 0.05) after HPD-300 resin purification. In addition, the extract exhibited a significant (P < 0.05) increase in total flavonoid contents, in vitro antioxidant (DPPH and FRAP) and antidiabetic (α-glucosidase and BSA-glycation inhibition) activity after HPD-300 resin treatment. These findings indicate that selection of resin and applying optimal purification condition is an appropriate economical approach for obtaining bioactive phenolic compounds from rice bran.     

高效液相色谱-二极管阵列联用技术同时检测苹果醋和甘蔗醋中11种酚类物质

利用高效液相色谱联合二极管阵列技术（HPLC-DAD）建立一种可以同时检测不同苹果醋和甘蔗醋中11种酚类物质的分析方法。使用OMNI Hubble C₁₈色谱柱（250 mm×4.6 mm,5μm）,流动相A为甲醇-甲酸（100∶2,v/v）,B为10 mmol/L磷酸氢二钾-磷酸水溶液（200∶1,v/v）,流速为1.0 m L/min,进样量为20μL,梯度洗脱,柱温为35℃,二极管阵列扫描波长范围210⁴00 nm。应用本方法可以检测出不同苹果醋和甘蔗果醋中香草醛、香豆素、绿原酸、咖啡酸、阿魏酸、对香豆酸、芹菜素、木犀草素、肉桂酸、山奈酚、芒柄花黄素等11种酚类物质,其中甘蔗醋中富含绿原酸等10种酚类物质成分。本方法回收率在84.1%¹18.2%之间,结果准确可靠,简单易行,适合用于各类果醋中酚类物质的分析检测。      

绿豆种子萌发过程中酚类化合物的动态变化

建立了同时测定绿豆醇提液中19种酚类化合物的高效液相色谱（High performance liquid chromatography,HPLC）方法,并结合标准品对绿豆种子萌发过程中19种酚类化合物进行了定性定量分析。结果表明,在绿豆种子萌发过程中,酚类化合物种类和总量均有所增加,其中没食子酸和咖啡酸含量随萌发时间的显著延长而增加;芦丁、黄豆苷元、山奈酚、p-香豆酸、阿魏酸和染料木素含量在第7 d有最大值,分别为1574.5、114.3、80.3、43.3、207.9和45.3μg/g;槲皮素含量在第4 d有最大值28.0μg/g;原儿茶酸、橙皮苷、肉桂酸和柚皮素含量在第5 d有最大值,分别为89.1、297.4、84.6和30.6μg/g;白藜芦醇、对硝基苯甲酸和鹰嘴豆芽素A含量在第2 d有最大值,分别为89.6、70.8和237.0μg/g;杨梅酮、儿茶素和橙皮素含量在第6 d有最大值,分别为40.7、712.1和58.2μg/g。而19种酚类化合物总含量在第7 d有最大值,达到3508.8μg/g。在绿豆种子萌发过程中,由于酶种类增多、活性增强,导致次级代谢产物大量合成,酚类化合物含量增多。      

响应面法优化超声辅助提取核桃叶多酚的工艺研究

首次以陇南核桃叶为原料,运用超声波辅助提取技术对核桃叶中的多酚类物质进行提取,在温度为40℃,超声功率为110W的条件下,考察了料液比、超声时间、乙醇浓度对多酚提取率的影响,并利用响应面法优化了提取工艺,得出最佳工艺参数为:料液比为1∶10,超声提取时间为10min,乙醇浓度为70%,在最佳提取工艺的条件下,核桃多酚的含量可达到4.04mg/g,为核桃叶多酚的进一步开发提供了依据。     

Identification and quantification of phenolic compounds from sunflower (Helianthus annuus L.) kernels and shells by HPLC-DAD/ESI-MS

Phenolic compounds were extracted from defatted sunflower (Helianthus annuus L.) kernels and shells and characterised by HPLC with diode array and electrospray ionisation (ESI) mass spectrometric detection in the negative mode. Quantification of individual compounds was carried out by external calibration. Among the eleven compounds analysed 5-O-caffeoylquinic acid was predominant amounting up to 59.1 mg/100 g in the shells and 3050.5 mg/100 g in the kernels. The specific fragmentation patterns of mono- and dihydroxycinnamoylquinic acids allowed the unambiguous distinction of several stereoisomers which have not been described for sunflower seeds and seed shells so far. The total phenolic content of about 4200 mg/100 g on a dry matter basis revealed defatted sunflower meal to be a promising source of phenolic compounds that might be recovered and used as natural antioxidants. Furthermore, the press residues originating from sunflower oil extraction were shown to be still rich in phenolic antioxidants, thus, providing the opportunity to valorize these by-products in terms of sustainable agricultural production.