SleepExplorer: a visualization tool to make sense of correlations between personal sleep data and contextual factors

Getting enough quality sleep is a key part of a healthy lifestyle. Many people are tracking their sleep through mobile and wearable technology, together with contextual information that may influence sleep quality, like exercise, diet, and stress. However, there is limited support to help people make sense of this wealth of data, i.e., to explore the relationship between sleep data and contextual data. We strive to bridge this gap between sleep-tracking and sense-making through the design of SleepExplorer, a web-based tool that helps individuals understand sleep quality through multi-dimensional sleep structure and explore correlations between sleep data and contextual information. Based on a two-week field study with 12 participants, this paper offers a rich understanding on how technology can support sense-making on personal sleep data: SleepExplorer organizes a flux of sleep data into sleep structure, guides sleep-tracking activities, highlights connections between sleep and contributing factors, and supports individuals in taking actions. We discuss challenges and opportunities to inform the work of researchers and designers creating data-driven health and well-being applications.     

Albumin stabilizes (–)‐epigallocatechin gallate in human serum: Binding capacity and antioxidant property

(–)‐Epigallocatechin gallate (EGCg) is the major component of green tea and is known to show strong biological activity, although it can be easily oxidized under physiological conditions. In this study, we indicate that EGCg is stable in human serum and that human serum albumin (HSA) stabilizes EGCg under aerobic condition. Although EGCg is usually decomposed within 1 h in aqueous solution at neutral pH, EGCg in serum and phosphate buffer (pH 7.4) containing HSA was stable over 1 h, even at neutral and slightly alkaline pH. Under these conditions, EGCg binds to HSA non‐covalently. The sulfhydryl group acts as an antioxidant for EGCg oxidation. Incubation of EGCg with HSA is accompanied by the oxidation of a free sulfhydryl group in HSA. These results suggest that the antioxidant property and the binding capacity of HSA contribute to the stabilization of EGCg in human serum.     

Preparation of highly dispersed gold nanoparticles inside the porous support assisted by amphiphilic vinyl maleate copolymer

An amphiphilic copolymer composed of maleic acid and alkyl (C₁₈) vinyl monomer was encapsulated into the porous support. A series of colloidal gold nanoparticles of known size was substantially immobilized in the composite porous supports based on cross-linked polyacrylate ester and cross-linked polystyrene resin. Maleic acid moiety of the amphiphilic copolymer can act as a stabilizer for gold nanoparticles in analogy to citric acid, whereas alkyl chains play a role for the stable accommodation of the amphiphilic copolymer. Maleic acid stabilizes the gold nanoparticles by flexing the geometrical arrangement of the linear polymer. Presence of C₁₈ alkyl chain in the poly(C₁₈-vinyl maleate) is indispensable to act as spacing group that prevents mutual aggregation of gold nanoparticles. On the other hand, gold nanoparticles with average diameter of less than 8 nm were spontaneously formed by treatment of the composite resin beads with aqueous HAuCl₄ solution, subsequently dispersed inside the pores of resin beads as observed by TEM. We have also elucidated the catalytic activity of the material with the hydrogenation of cinnamaldehyde in supercritical carbon dioxide. Notably, apparent size effect of gold was observed in the selectivity of the reaction.     

DNA taken into Bacillus subtilis competent cells by lysed-protoplast transformation is not ssDNA but dsDNA

Competent Bacillus subtilis incorporates whole-genome DNA (4215 kb) from the protoplast lysate of B. subtilis subtilis [Akamatsu, T. and Taguchi, H., Biosci. Biotechnol. Biochem., 65, 823-829 (2001)]. A continuous incorporated DNA is longer than 1500 kb [J. Biosci. Bioeng., 101, 257-262 (2006)]. Whether the incorporated DNA is single-stranded (ssDNA) or double-stranded DNA (dsDNA) has been studied by examining the transforming activity of the incorporated DNA. B. subtilis BEST7027 was used as the donor strain, which has a heterologous region consisting of the 145 kb region of the Synechocystis sp. PCC6803 genome and erm gene. The donor DNA was transferred to a wild-type or a recA recipient strain (AYG2 or SYN9), and protoplast lysate was prepared from the transformants and used as the donor DNA source for the second recipient strain (AU1 or AV1). The intergenote region showed a significant transforming activity. When DNase I was added to both cells collected from the first transformation mixture and the following protoplastization, the result was similar to that obtained without DNase I. All of the observations strongly suggest that the incorporated DNA is dsDNA, and the transformation of competent B. subtilis by DNA in protoplast lysate is different from that by purified DNA taken up conventionally.     

Reduction of Second-Order Temperature Dependence of Athermal AWG With Resin-Filled Groove by Pressure Control

In this letter, we have developed a novel technique for improving the temperature stability of an athermal silica-based arrayed-waveguide grating with a resin-filled groove. We compensated for the residual second-order temperature dependence of the passband wavelength using the pressure-induced refractive index change in a resin inserted into the optical paths. Pressure control under the temperature variation was achieved by using a structure composed of an optical fiber piston and bimetal actuators. We demonstrated that the passband wavelength variation was successfully reduced from 0.080 to 0.025 nm in a -40degC - 85degC temperature range, without any insertion loss increase or spectral change.     

Effect of culture conditions of acetic acid bacteria on cellulose biosynthesis

An attempt was made to clarify the effect of culture conditions of an acetic acid bacteria (Acetobacter xylinum) on cellulose biosynthesis using glucose as carbon source in complex medium. Synchronous culture conditions were first realised on cellulose biosynthesis by cooling the system to 4°C for 24 h. Under the synchronous conditions stepwise division of the cell and the stepwise production of cellulose were found. Furthermore, cellulose was proved to be produced when the cell number in the medium was constant.     

Potent Activation of Human but Not Mouse TRPA1 by JT010

Transient receptor potential (TRP) ankyrin repeat 1 (TRPA1), which is involved in inflammatory pain sensation, is activated by endogenous factors, such as intracellular Zn²⁺ and hydrogen peroxide, and by irritant chemical compounds. The synthetic compound JT010 potently and selectively activates human TRPA1 (hTRPA1) among the TRPs. Therefore, JT010 is a useful tool for analyzing TRPA1 functions in biological systems. Here, we show that JT010 is a potent activator of hTRPA1, but not mouse TRPA1 (mTRPA1) in human embryonic kidney (HEK) cells expressing hTRPA1 and mTRPA1. Application of 0.3–100 nM of JT010 to HEK cells with hTRPA1 induced large Ca²⁺ responses. However, in HEK cells with mTRPA1, the response was small. In contrast, both TRPA1s were effectively activated by allyl isothiocyanate (AITC) at 10–100 μM. Similar selective activation of hTRPA1 by JT010 was observed in electrophysiological experiments. Additionally, JT010 activated TRPA1 in human fibroblast-like synoviocytes with inflammation, but not TRPA1 in mouse dorsal root ganglion cells. As cysteine at 621 (C621) of hTRPA1, a critical cysteine for interaction with JT010, is conserved in mTRPA1, we applied JT010 to HEK cells with mutations in mTRPA1, where the different residue of mTRPA1 with tyrosine at 60 (Y60), with histidine at 1023 (H1023), and with asparagine at 1027 (N1027) were substituted with cysteine in hTRPA1. However, these mutants showed low sensitivity to JT010. In contrast, the mutation of hTRPA1 at position 669 from phenylalanine to methionine (F669M), comprising methionine at 670 in mTRPA1 (M670), significantly reduced the response to JT010. Moreover, the double mutant at S669 and M670 of mTRPA1 to S669E and M670F, respectively, induced slight but substantial sensitivity to 30 and 100 nM JT010. Taken together, our findings demonstrate that JT010 potently and selectively activates hTRPA1 but not mTRPA1.