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The orientation factor by infrared dichroism of polypropylene film was compared with the other orientation factors. After the correction of imperfect polarization and the suitable choice of the base line, it was found that infrared dichroism is a reliable indicator of the orientation factor. Orientation factors obtained from the 998 and 842 cm.?1 bands agreed with the x-ray orientation factor, and that from the 975 cm.?1 band agreed with the optical orientation factor.  相似文献   
95.
    
A ceramide deficiency in the stratum corneum (SC) is an essential etiologic factor for the dry and barrier-disrupted skin of patients with atopic dermatitis (AD). Previously, we reported that sphingomyelin (SM) deacylase, which hydrolyzes SM and glucosylceramide at the acyl site to yield their lysoforms sphingosylphosphorylcholine (SPC) and glucosylsphingosine, respectively, instead of ceramide and/or acylceramide, is over-expressed in AD skin and results in a ceramide deficiency. Although the enzymatic properties of SM deacylase have been clarified, the enzyme itself remains unidentified. In this study, we purified and characterized SM deacylase from rat skin. The activities of SM deacylase and acid ceramidase (aCDase) were measured using SM and ceramide as substrates by tandem mass spectrometry by monitoring the production of SPC and sphingosine, respectively. Levels of SM deacylase activity from various rat organs were higher in the order of skin > lung > heart. By successive chromatography using Phenyl-5PW, Rotofor, SP-Sepharose, Superdex 200 and Shodex RP18-415, SM deacylase was purified to homogeneity with a single band of an apparent molecular mass of 43 kDa with an enrichment of > 14,000-fold. Analysis by MALDI-TOF MS/MS using a protein spot with SM deacylase activity separated by 2D-SDS-PAGE allowed its amino acid sequence to be determined and identified as the β-subunit of aCDase, which consists of α- and β-subunits linked by amino bonds and a single S-S bond. Western blotting of samples treated with 2-mercaptoethanol revealed that, whereas recombinant human aCDase was recognized by antibodies to the α-subunit at ~56 kDa and ~13 kDa and the β-subunit at ~43 kDa, the purified SM deacylase was detectable only by the antibody to the β-subunit at ~43 kDa. Breaking the S-S bond of recombinant human aCDase with dithiothreitol elicited the activity of SM deacylase with ~40 kDa upon gel chromatography. These results provide new insights into the essential role of SM deacylase expressed as an aCDase-degrading β-subunit that evokes the ceramide deficiency in AD skin.  相似文献   
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The tensile behaviors of drawn polystyrene (PS) and high-impact polystyrene (HIPS) were examined systematically in the wide range of strain rate, 1.7 × 10?4–13.1 m/s, without changing the mode of deformation and the shape of the test pieces. It was found for both PS and HIPS that the flexion points of birefringence with increase of draw ratio are intimately correlated with the tensile properties. Especially, the breaking strain and energy of undrawn and drawn HIPS were assigned to the contributions of the following three toughening mechanisms: (a) the generation of large numbers of microcrazes from the equatorial zone of the dispersed rubber particles; (b) the extension of interfacial rubber phase around the circumpolar zone of the dispersed rubber particles and its disintegration from the matrix component; and (c) the shear band formation of the matrix component followed by cold drawing. These mechanisms were discussed in connection with the factors of stress concentration to the rubber particles, hydrostatic pressure effect arising from the difference in Poisson's ratio of rubber and matrix components, and heat generation due to the adiabatic deformation.  相似文献   
98.
Pole‐mounted transformers are especially vulnerable to lightning damage. The progress of the information society imposes increasingly stringent requirements for the reliability of electric power supply, and this in turn necessitates a reduction in lightning damage to pole‐mounted transformers. Lightning protective devices (surge arresters) are now being installed around the primary bushing of the transformers, which has decreased the number of disconnections around the primary bushing due to lightning. But surge arresters installed on the primary side of the transformer cannot protect it against backflow lightning entering the secondary side of the transformer. The characteristic of transformer damage by backflow lightning is that the electromagnetic force produced by the current flowing into the secondary side deforms the transformer windings. This paper elucidates the mechanism of transformer damage by lightning flowing into the secondary side by comparing actual lightning damage cases with the results of verification tests using a short‐circuit generator. Effective countermeasures against transformer damage by backflow lightning are examined by EMTP calculations, which indicate that neutral grounding on the low‐voltage distribution line is the most effective way of decreasing the current flowing into the transformer. The lower the grounding resistance, the less current flows into the transformer. In addition, decreasing the voltage on the secondary side is important in order to protect the secondary‐side bushing. The calculation results indicate that surge arresters installed around the secondary side of the transformer are effective in decreasing the voltage on the secondary side. © 2010 Wiley Periodicals, Inc. Electr Eng Jpn, 171(2): 1–11, 2010; Published online in Wiley InterScience ( www.interscience. wiley.com ). DOI 10.1002/eej.20921  相似文献   
99.
A significant immunomodulating activity was found in the hot-water-soluble extract of an edible brown seaweed, Hijikia fusiforme (Hijiki in Japanese) which showed an enhancing activity for the proliferative response of spleen cells in endotoxin-nonresponder C3H/HeJ mice. This activity was separated into polysaccharide and nonpolysaccharide fractions. The former fraction exhibited a much higher activity than that of the latter fraction. The enhancing effect of the polysaccharide fraction on the proliferative response of spleen cells was associated with the response of the B cell population, but not with that of the T cell population judged by experiments using nylon wool column-purified T cells and antisera against B cell- or T-cell-specific antigens. The active component of the polysaccharide fraction was further fractionated using DEAE cellulose column chromatography which also caused enhancing effects on polyclonal antibody (IgM and IgG) production and the release of interleukin-1α or tumour necrosis factor-α from peritoneal macrophages of C3H/HeJ mice. In addition, these immunomodulating activities of the polysaccharide fraction were associated with the polysaccharides themselves, but not with the artificial activities induced by contaminated endotoxins. The biochemical and physicochemical properties of the immunoenhancing polysaccharides were partially characterised and the significance of the present finding is discussed from the viewpoint of the immunopotentiating activity of edible seaweeds against carcinogenesis. © 1998 SCI.  相似文献   
100.
Under a nonuniform field in an atmospheric short gap, a branched pulse streamer corona changes to a single filamentary discharge (SFD) for a split second just before sparkover occurs. This SFD is a pretransitional phenomenon of sparkover. Therefore, it is very important to study SFD for an understanding of the sparkover mechanism. In this work, the SFD was recreated well by applying a highly repetitive impulse voltage to a needle‐plane gap in synthetic air with dilute CO2. The characteristics of the SFD, including the channel temperature, the propagation of the streamer head, and the formation process, were investigated by means of an intensified charge coupled‐device (ICCD) camera and a spectroscopic measurement system. It was found that the transition to SFD progresses with localized gas heating along the discharge channel. Furthermore, it was revealed that the SFD is a nonbranched positive streamer.  相似文献   
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