Comparative Analysis of Platelet-Derived Extracellular Vesicles Using Flow Cytometry and Nanoparticle Tracking Analysis

Growing interest in extracellular vesicles (EVs) has prompted the advancements of protocols for improved EV characterization. As a high-throughput, multi-parameter, and single particle technique, flow cytometry is widely used for EV characterization. The comparison of data on EV concentration, however, is hindered by the lack of standardization between different protocols and instruments. Here, we quantified EV counts of platelet-derived EVs, using two flow cytometers (Gallios and CytoFLEX LX) and nanoparticle tracking analysis (NTA). Phosphatidylserine-exposing EVs were identified by labelling with lactadherin (LA). Calibration with silica-based fluorescent beads showed detection limits of 300 nm and 150 nm for Gallios and CytoFLEX LX, respectively. Accordingly, CytoFLEX LX yielded 40-fold higher EV counts and 13-fold higher counts of LA⁺CD41⁺ EVs compared to Gallios. NTA in fluorescence mode (F-NTA) demonstrated that only 9.5% of all vesicles detected in scatter mode exposed phosphatidylserine, resulting in good agreement of LA⁺ EVs for CytoFLEX LX and F-NTA. Since certain functional characteristics, such as the exposure of pro-coagulant phosphatidylserine, are not equally displayed across the entire EV size range, our study highlights the necessity of indicating the size range of EVs detected with a given approach along with the EV concentration to support the comparability between different studies.     

Interfacing perovskite strontium molybdate to molybdenum disulfide nanoplatelts for boosting HER from water

Due to low hydrogen adsorption free energy at the edges of 2D-MoS₂ layered sheets, nanostructured MoS₂ materials recently are assigned to prospective electrocatalysts for hydrogen evolution reaction (HER) from water. However, the efficiency and stability of HER onto the MoS₂ designed on the conductive substrates are poor. To significantly increase the number of active sites and achieve a long-time working stability, the design of hybrid-type electrodes is crucial. Here, we report the synthesis of a new hybrid material composed of molybdenum disulfide and molybdenum oxides heterostructured with strontium molybdate. For this, a facile one-pot hydrothermal process was developed directly onto the TiO₂ nanotube carpet substrate. The interfacing of strontium molybdate at the electrode substrate verified by X-ray photoelectron spectroscopy and Time of flight secondary ions mass spectrometry (ToF SIMS) techniques. Considerable higher catalytic activity at the surface of this hybrid film, with the onset potential of 190 mV vs RHE and a Tafel slope of 66 mV dec^?1 attaining ~80 mA cm^?2 at 0.35 V overvoltage was ascertained. Exciting HER stability in comparison with the pure synthetic MoS₂ was verified by a prolonged potential cycling from 0.05 to ?0.35 V versus RHE potential and 45 h continuous HER processing at a constant current density.     

Unpatterned Bioactive Poly(Butylene 1,4-Cyclohexanedicarboxylate)-Based Film Fast Induced Neuronal-Like Differentiation of Human Bone Marrow-Mesenchymal Stem Cells

Herein, we present poly(butylene 1,4-cyclohexanedicarboxylate) (PBCE) films characterized by an unpatterned microstructure and a specific hydrophobicity, capable of boosting a drastic cytoskeleton architecture remodeling, culminating with the neuronal-like differentiation of human bone marrow-mesenchymal stem cells (hBM-MSCs). We have used two different filming procedures to prepare the films, solvent casting (PBCE) and compression-moulding (PBCE*). PBCE film had a rough and porous surface with spherulite-like aggregations (Ø = 10–20 μm) and was characterized by a water contact angle = 100°. PBCE* showed a smooth and continuous surface without voids and visible spherulite-like aggregations and was more hydrophobic (WCA = 110°). Both surface characteristics were modulated through the copolymerization of different amounts of ether-oxygen-containing co-units into PBCE chemical structure. We showed that only the surface characteristics of PBCE-solvent-casted films steered hBM-MSCs toward a neuronal-like differentiation. hBM-MSCs lost their canonical mesenchymal morphology, acquired a neuronal polarized shape with a long cell protrusion (≥150 μm), expressed neuron-specific class III β-tubulin and microtubule-associated protein 2 neuronal markers, while nestin, a marker of uncommitted stem cells, was drastically silenced. These events were observed as early as 2-days after cell seeding. Of note, the phenomenon was totally absent on PBCE* film, as hBM-MSCs maintained the mesenchymal shape and behavior and did not express neuronal/glial markers.     

Assessing the capability of broadband indices derived from Landsat 8 Operational Land Imager to monitor above ground biomass and salinity in semiarid saline environments of the Bahía Blanca Estuary,Argentina

In arid and semi-arid ecosystems, salinisation and desertification are the most common processes of land degradation, and satellite data may provide a valuable tool to assess land surface condition and vegetation status. The aim of this study was to evaluate the capability of Landsat 8 OLI (Operational Land Imager) remote sensing information and broadband indices derived from it, to monitor above ground biomass (AGB) and salinity in two different semiarid saline environments (unit a and unit b) in the Bahía Blanca Estuary. Unit a (Ua) is composed of bushes of Cyclolepis genistoides in association with Atriplex undulata and 41% of bare soil. Unit b (Ub) is composed of dense thickets of Allenrolfea patagonica in association with C. genistoides and 34% of bare soil. Pearson’s correlation analyses were performed between field estimates of AGB and salinity (soil salinity and interstitial water salinity) and remote sensing estimates. Satellite data include surface reflectance of individual bands, vegetation indices (NDVI [normalised difference vegetation index], SAVI [soil-adjusted vegetation index], MSAVI2 [modified soil-adjusted vegetation index], NDII [normalised difference infrared index], GNDVI [green normalised difference vegetation index], GRNDI [green-red normalised difference index], OSAVI [optimised soil-adjusted vegetation index], SR [simple ratio]), and salinity indices (SI1, SI2, SI3 [salinity index 1, 2 and 3, respectively] and BI [brightness index]). Correlation analyses involving AGB were performed twice; first considering all months and then again excluding the months with higher soil salinities. In Ua, soil adjusted vegetation indices SAVI and MSAVI2 showed to be suitable to detect changes in the total green AGB and C. genistoides green AGB (the major contributor to total green AGB). After excluding data from December and January (the months with the highest soil salinity), green AGB of A. undulata also showed a significant positive correlation with soil adjusted indices SAVI, MSAVI2 and OSAVI. Although proportionally this species was not a large contributor to the total biomass, it is characterised by a high leaf reflectance, which makes it suitable for biomass retrieval. In Ub, significant positive correlations were obtained between NDVI, SAVI, NDII, OSAVI and SR indices and the AGB green ratio, but significant negative correlations were obtained between A. patagonica red AGB and these vegetation indices. When December and January were excluded from the analysis the negative correlations between vegetation indices NDVI, OSAVI and SR and red AGB remained significant (r = ?0.68, ?0.76 and ?0.7, respectively). The positive correlations between these indices and AGB green ratio (r = 0.73, 0.78 and 0.75, respectively) remained significant as well. Significant negative correlations were also found between NDVI, NDII, GNDVI, OSAVI and SR indices and field salinity estimates. As soil salinisation induces A. patagonica reddening, red AGB and soil salinity covariate in the field, and the negative correlation with vegetation indices may be useful to retrieve information on both variables combined, which are indicative of water stress. Correlation analysis between field estimates of salinity and spectral salinity indices showed significant positive correlation for all the tested indices. The obtained results highlight the importance of a thoughtful selection of remote sensing indices to account for changes in vegetation biomass, especially in arid and semiarid environments particularly sensitive to desertification and salinisation. Also, ground truth cannot be overlooked, and field work is necessary to test index performance in every case.     

Parallel SUMIS soft detector for large MIMO systems on multicore and GPU

The Journal of Supercomputing - The number of transmit and receiver antennas is an important factor that affects the performance and complexity of a MIMO system. A MIMO system with very large...