Validation of an inhibition ELISA using a monoclonal antibody for foot-and-mouth disease (FMD) primary diagnosis

An inhibition ELISA (IH-ELISA) test for foot-and-mouth disease virus (FMDV) was validated using 106 epithelial samples from suspected cases of FMD in Argentina submitted to the Argentine National Diagnostics Laboratory (GELAB) over a period of 12 months and examined in parallel with the complement fixation test (CFT). IH-ELISA was found to be more sensitive, detecting 25% (26 samples) more FMDV positives than the CFT in original suspensions of field samples. The effect of storage conditions on 12S stability was examined. Plates stored at 4 degrees C blocked with 1% ovalbumin and plates stored at -20 degrees C with or without blocking buffer could be used for at least 90 days. When various brands of polystyrene plates were compared for 12ps adsorption it was found that those microplates of higher binding capacity were more efficient.     

Envelope glycoprotein gp120 of human immunodeficiency virus type 1 alters ion transport in astrocytes: implications for AIDS dementia complex

Infection by human immunodeficiency virus type 1 (HIV-1) is often complicated by a variety of neurological abnormalities. The most common clinical syndrome, termed acquired immunodeficiency syndrome (AIDS) dementia complex, presents as a subcortical dementia with cognitive, motor, and behavioral disturbances and is unique to HIV-1 infection. The pathogenesis of this syndrome is poorly understood but is believed to involve interactions among virally infected macrophages/microglia, astrocytes, and neurons. In this study, we show that exposure of primary rat and human astrocytes to heat-activated HIV-1 virions, or to eukaryotically expressed HIV-1 and HIV-2 envelope glycoproteins (gp120) stimulates amiloride-sensitive Na+/H+ antiport, potassium conductance, and glutamate efflux. These effects are blocked specifically by amiloride, an inhibitor of Na+/H+ antiport and by the selective removal of gp120 with immobilized monoclonal antibody. As a result of modulation of astrocytic function by gp120, the ensuing neuronal depolarization and glutamate exposure could activate both voltage-gated and N-methyl-D-aspartate-regulated Ca2+ channels, leading to increases in intraneuronal Ca2+ and neuronal death. These findings implicate the astrocyte directly in the pathogenesis of AIDS dementia complex.     

编码器自动监测与故障诊断系统的研究与实现

文中介绍了某大型机电设备编码器的实时监测与故障诊断系统的设计工作.系统采用在线和离线两种工作方式,实现了接触式编码器的监测和诊断.系统硬件主要电路板基于PC104总线结构,18路I/O通道(9路输入,9路输出)完成9位编码器的检测和诊断.系统软件界面友好,巧妙地实现了格雷码与角度值的转换.本系统对其他编码器自动监测和故障诊断系统具有重要的借鉴意义.     

形势要求机床工具工业必须加快增长方式的转型(一)

1999年以来，我国国民生产总值持续快速稳定增长。表1是这期间国民生产总值增长情况。     

成都市彭青公路后张法预应力混凝土空心梁板施工技术研究

成都市彭青公路长25．83km,采用后张法预应力混凝土空心梁板施工,在施工中通过关注混凝土浇筑和钢绞线张拉两大关键技术。提出了自己的见解。     

化地不化人的城市化符合中国国情吗?——人口密集型的“老浦西”和土地资本密集型的“新浦东”的历史比较

基于对城市化真谛的理解,在论证城市化的二重性和城市集聚效应的内生性的基础上,针对中国城市化过程中存在的化地不化人的弊病,在对比"老浦西"和"新浦东"城市化机制及其绩效后发现,"新浦东"受制于现行户籍和土地制度,对人口、土地和资本等要素的配置以行政导向为主,以土地密集和资本密集为主要特征,对中国真实城市化率的提高贡献甚微,陷入城市自我现代化的困境,也违反国情和环保的要求,难以持续。对众多农村人口亟待城市化的中国而言,"新浦东"模式不具备普遍意义。市场导向的人口密集型的"老浦西"模式,更符合内生增长的要求,更能发挥城市吸收农村人口的集聚效应,也更符合可持续增长和环保原则,值得今日中国借鉴。     

都汶高速公路庙子坪岷江特大桥震后5号主墩加固技术

都汶高速公路是成都通往汶川的重要的交通枢纽,为国道213和317合线。都汶高速公路庙子坪岷江特大桥(又名紫坪铺大桥)直接跨越紫坪铺水库和岷江,都江堰端接著名的董家山高瓦斯隧道,汶川端接龙洞子隧道。5.12汶川大地震对庙子坪岷江特大桥产生了不少震害,地震后大桥的加固时间紧、工作量大、技术复杂,这在中国乃至世界桥梁史上前所未有;特别是5号主墩加固异常复杂和艰难,从桥面防撞护栏顶面到5号主墩地面承台顶面之间总体落差114.488 m,5号主墩地面承台顶面到水面水深达65 m。通过对5号主墩承台顶面以上14 m加固区间(全部位于深水区域)的潜水堵孔和钢沉箱工艺进行深入细致的研究,制定了系列相应措施。