Cyclic variations in the permeability of the cell wall of Saccharomyces cerevisiae.

To study cell-cycle-related variations in wall permeability of Saccharomyces cerevisiae, two approaches were used. First, an asynchronous culture was fractionated by centrifugal elutriation into subpopulations containing cells of increasing size. The subpopulations represented different stages of the cell cycle as judged by light microscopy. Cell wall porosity increased when these subpopulations became enriched with budded cells. Secondly, synchronous cultures were obtained by releasing MATa cells from alpha-factor induced G1-arrest. These cultures grew synchronously for at least two generations. The cell wall porosity increased sharply in these cultures, shortly before buds became visible and was maximal during the initial stages of bud growth. It decreased in cells which had completed nuclear migration and before abscission of the bud had occurred. The porosity reached its lowest value during abscission and in unbudded cells. We examined the incorporation of mannoproteins into the wall during the cell cycle. SDS-extractable mannoproteins were incorporated continuously. However, the incorporation of glucanase-extractable mannoproteins, which are known to affect cell wall porosity, showed cyclic oscillations and reached its maximum after nuclear migration. This coincided with a rapid decrease in cell wall porosity, indicating that glucanase-extractable mannoproteins might contribute to this decrease.     

浅谈赣抚大堤樟防1标射水造墙的接缝技术和处理措施

介绍射水造墙的接缝原理，并把自己在施工过程中为确保接缝质量达到设计要求而总结出一些经验和处理措施提出为一起探讨。     

Cleanbot-Ⅰ擦窗机器人的智能化技术

本文简述了擦窗机器人智能化的主要概念．介绍了Ｃｌｅａｎｂｏｔ－Ｉ擦窗机器人系统的结构特点、主要组成部分、工作原理，操作方式，未知局部环境的模型建立，各类内、外传感器的结构、原理和信息融合技术，以及路径规划的方法．     

泄压注浆法在治理小型突水中的运用

七煤集团建设工程公司在施工辽阳铧子石膏矿主、风井井筒时 ,多次采用泄压注浆法成功通过中小型含水层 ,对小型突水进行了有效封堵 ,取得了较好的经济效益     

Generation of adaptive gait patterns for quadruped robot with CPG network including motor dynamic model

This paper describes the generation of adaptive gait patterns using new Central Pattern Generators (CPGs) including motor dynamic models for a quadruped robot under various environments. The CPGs act as the flexible oscillators of the joints and adjust joint angles to required values. The CPGs are interconnected with each other and sets of their coupling parameters are adjusted by a genetic algorithm so that the quadruped robot can realize stable and adequate gait patterns. Generation of gait patterns results in the formation of the CPG networks suitable for the formation of not only a straight walking pattern but also of rotating gait patterns. Experimental results demonstrate that the proposed CPG networks are effective for the automatic adjustment of the adaptive gait patterns for the tested quadruped robot under various environments. Furthermore, the target tracking control based on image processing is achieved by combining the general gait patterns. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 155(1): 35–43, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20225     

G104-5P42分支水平井钻井液工艺技术

G104-5P42井是在高尚堡G104-5区块构造较高部位部署的一口重点开发井，也是冀东油田第一口鱼骨型水平分支井，钻探目的是开发高浅北区Ng12油层，挖掘高浅北区井间剩余油潜力。该井主井眼斜深为2388m，水平位移为736．42m，水平段长243m，第一分支斜深为2342m，水平段长120．25m，水平位移为682．14m，第二分支斜深为2421m，水平段长117m，水平位移为761．73m。三开选用无固相甲酸盐聚合醇钻井液，在钻进油气层前加入GN-YBJ-I保护剂。应用结果表明，无固相甲酸盐聚合醇钻井液具有好的防塌能力和润滑减阻效果，性能稳定、黏度、切力较低，有利于减少环空压力损耗；在油气层段密度控制在1．08g／cm^3以内，为近年来同区块密度使用最低井，减小了有害固相及滤液对储层的伤害密度，经测试该井获高产油流；该井全井平均机械钻速为15．09m／h，钻井周期比原设计提前15d。该体系的配制及维护成本较高，适宜在目的层井段使用，但可回收再利用。     

皮革去污上光用乳化蜡的研制

采用石蜡、微晶蜡为主要原料 ,经实验选出了以水为稀释剂的乳化型皮革去污上光蜡。实验优化的配方为石蜡 12 .5 g ,微晶蜡 8.0 g ,硬脂酸 8.5g ,三乙醇胺 5 .5 g ,水量 70 (涂用 )或 130 g(喷用 )。反应条件为乳化时间 4 0min ,搅拌速度 5 0 0～ 70 0r/min ,乳化温度 90℃。制得上光剂产品的去污性能可与用去污剂单独处理的效果相当 ,亮度可达 6 6 .7。     

用瑞利波检验高喷防渗墙的施工质量及地层分布特征

本文阐述了瞬态多道瑞利波探测的原理和方法，并将其应用到大堤高喷防渗墙施工质量的检验中，取得一定成效，测试结果与测压管观测结果对比，相互吻合，为类似工程的质量检验提供了一种可采用的检测手段。     

THE STICKY ISSUES OF DRYING

Unconrolled agglomeration of particles and adhesion of the particulate mass to wall surfaces are common severe problems in many drying operations. This paper reviews the mechanisms of interparticle and particle-surface attraction as well as the theory and the existing tests for characterising powders in terms of internal strength, wall friction and adhesion. The usefulness of the information obtained from these tests in dryer design and operation is critically reviewed. The importance of powder stickiness in various types of dryers is discussed and established methods of combating the problem are presented Recommendations an made for further research in the area of powder cohesion and adhesion.