Three-dimensional silk impregnated HAp/PHBV nanofibrous scaffolds for bone regeneration |
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| Authors: | Oylum Colpankan Gunes Irem Unalan Berivan Cecen Hasan Havitcioglu Ozcan Ustun |
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| Affiliation: | 1. Metallurgical and Materials Engineering Department, Faculty of Engineering, Dokuz Eylul University, Buca, Izmir, Turkey;2. Biomedical Technologies Department, Graduate School of Natural and Applied Sciences, Dokuz Eylul University, Buca, Izmir, Turkey;3. Biomechanics Department, Institute of Health Science, Dokuz Eylul University, Inciralt?, Izmir, Turkey;4. Orthopedics and Traumatology Department, Faculty of Medicine, Dokuz Eylul University, Inciralt?, Izmir, Turkey;5. Histology and Embryology Department, Faculty of Medicine, Dokuz Eylul University, Inciralt?, Izmir, Turkey |
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| Abstract: | Three-dimensional silk fibroin impregnated poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibrous scaffolds with or without hydroxyapatite (HAp) were prepared by wet-electrospinning method followed by freeze-drying. Scaffolds with cotton wool-like structure have the average fiber diameter of 450–850?nm with 80–85% porosity. In-vitro cell culture tests using MG-63 osteosarcoma human cells revealed improved cell viability, alkaline phosphatase (ALP) activity and total cellular protein amount on the silk impregnated scaffolds compared to PHBV and HAp/PHBV scaffolds after 10 days of cell culture. Immunohistochemical analyses on the silk impregnated scaffolds showed that HAp triggered cell penetration and type I collagen production. Besides, HAp mineralization tendency increased with a decrease in percent crystallinity of the scaffolds comprising HAp and silk after 4 weeks of incubation in simulated body fluid. Consequently, cotton wool-like HAp/PHBV-SF scaffold would be a promising candidate as a bone-filling material for tissue regeneration. |
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| Keywords: | Bone tissue engineering hydroxyapatite PHBV silk fibroin wet-electrospinning |
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