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Nano-hydroxyapatite and nano-hydroxyapatite/zinc oxide scaffold for bone tissue engineering application |
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| Authors: | Fatemeh Heidari Reza Bazargan-Lari Mehdi Razavi Farahnaz Fahimipour Daryoosh Vashaee Lobat Tayebi |
| Affiliation: | 1. Department of Materials Engineering, School of Engineering, Yasouj University, Yasuj, Iran;2. Department of Materials Science and Engineering, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran;3. BiionixTM (Bionic Materials, Implants & Interfaces) Cluster, Department of Internal Medicine, College of Medicine, University of Central Florida, Orlando, FL, USA Department of Materials Science & Engineering, University of Central Florida, Orlando, FL, USA;4. Marquette University School of Dentistry, Milwaukee, WI, USA Dental Biomaterials Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran;5. Department of Electrical and Computer Engineering, NC State University, Raleigh, NC, United States;6. Marquette University School of Dentistry, Milwaukee, WI, USA |
| Abstract: | This research aims to evaluate the mechanical properties, biocompatibility, and degradation behavior of scaffolds made of pure hydroxyapatite (HA) and HA-modified by ZnO for bone tissue engineering applications. HA and ZnO were developed using sol-gel and precipitation methods respectively. The scaffolds properties were characterized using X-ray diffraction (XRD), Fourier transform spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), atomic absorption (AA), and atomic force microscopy (AFM). The interaction of scaffold with cells was assessed using in vitro cell proliferation and alkaline phosphatase (ALP) assays. The obtained results indicate that the HA/ZnO scaffolds possess higher compressive strength, fracture toughness, and density—but lower hardness—when compared to the pure HA scaffolds. After immersing the scaffold in the SBF solution, more deposited apatite appeared on the HA/ZnO, which results in the rougher surface on this scaffold compared to the pure HA scaffold. Finally, the in vitro biological analysis using human osteoblast cells reveals that scaffolds are biocompatible with adequate ALP activity. |
| Keywords: | bioceramics bone nanomaterials |