化妆品抗粉刺(祛痘)的抑菌效果评价

研究了10种祛痘类化妆品对痤疮致病菌的体外抑菌活性。随机选用两类共10种祛痘类化妆品,根据使用方法选用悬液法定量和抑菌环法定性检测化妆品对金黄色葡萄球菌和痤疮丙酸杆菌的抑菌活性。结果表明:5种祛痘洁面乳中有3种洁面乳的抑菌率大于50%,有较好的抑菌作用;5种祛痘膏霜中有3种的抑菌环直径大于7 mm,有较好的抑菌效果,两类产品的合格率均为60%。     

两种含二茂铁基schiff 碱的合成及其抑菌活性

通过乙酰基二茂铁与两种杂环胺(4-氨基安替比林,2-氨基-5-巯基-1,3,4-噻二唑)缩合合成了两种含二茂铁基的Schiff碱(a、b),产率分别为85.8%和72.6%,并通过IR、1HNMR及元素分析对其结构进行了确证。最后,将合成出的两种Schiff碱分别对3种细菌(金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌)进行了初步抑菌实验,将其结果与非杂环类Schiff碱Fc-C(CH3)NC6H5=(c)的抑菌性能做了比较。结果表明,Schiff碱对上述3种细菌都有抑制作用,且抑菌效果随着Schiff碱浓度的增大而增强。此外,杂环类Schiff碱(a、b)对上述3种细菌的抑菌活性明显优于非杂环类Schiff碱(c)。     

微生物作用下多环芳烃在土壤中的迁移特征

以菲、蒽和芘为研究对象,通过淋溶实验考察抑菌和未抑菌条件下三者在土壤中的迁移过程,结果表明,微生物的存在抑制PAHs淋出:抑菌条件下180 d时菲、蒽和芘的淋出率分别为8.74%、28.53%和13.56%,未抑菌条件下240 d时菲、蒽和芘的淋出率分别为0.95%、1.47%和0.37%。抑菌和未抑菌条件下3种PAHs均易吸附于表层土壤中:淋滤240 d时,约75%的菲分布在进水端0~2 cm;超过90%的蒽分布在进水端10 cm以内;85%的芘分布在4 cm以内;微生物通过阻滞作用和降解作用影响PAHs的迁移,菲、蒽和芘在土壤中都能被土著微生物降解;60 d以前土柱中PAHs降解以好氧降解为主,60 d以后,PAHs的生物降解转为厌氧降解。     

粤蛇葡萄及几种杀菌药剂对柑橘溃疡病菌的抑茵活性

采用带药平板涂布法测定了粤蛇葡萄甲醇提取物及5种杀菌药剂对柑橘溃疡病菌的抑菌活性.结果表明:粤蛇葡萄茎和叶提取物对柑橘溃疡病菌均有抑菌活性,最低抑菌质量浓度(MIC)分别为4、2 g/L;杀菌药剂60%甲硫·异菌脲WP、72%农用链霉素SP、硫酸铜晶体、53.8%氢氧化铜WG和64%吧霜灵WP对柑橘溃疡病菌的最低抑菌浓度分别为10、2.5、0.625、0.3125、0.1563 g/L.采用液-液萃取法对粤蛇葡萄的抑菌活性成分进行了初步分离,结合活性测定,发现抑菌活性成分主要存在于茎和叶提取物的乙酸乙酯萃取物中.     

N-苯磺酰基-3-酰基吲哚苯甲酰腙类化合物的抑菌活性

[目的]研究N-苯磺酰基-3-酰基吲哚苯甲酰腙类化合物中具有潜在应用价值的抑菌先导化合物。[方法]在0.1 g/L质量浓度下,采用菌丝生长速率法分别测定了21种N-苯磺酰基-3-酰基吲哚苯甲酰腙类化合物1~21对小麦赤霉病菌、白菜黑斑病菌、烟草赤星病菌、棉花枯萎病菌和水稻稻瘟病菌的室内抑菌活性。[结果]化合物1、2、4、10和17对5种植物病原真菌均表现出较好的抑菌活性;如化合物4,抑菌率分别为51.70%、63.20%、54.30%、44.10%和45.20%,表现出广谱抑菌活性;尤其是对白菜黑斑病菌的抑菌率高达63.20%,超过阳性对照霉灵(52.60%)的抑菌活性。[结论]化合物1、2、4、10和17对所测植物病原真菌均表现出较好的抑菌活性,初步拟定将5个化合物作为进一步衍生修饰的先导化合物。     

几种表面活性剂的抑菌效果及与苯氧乙醇相互作用研究

通过测定3种常用的表面活性剂(PLANTACARE 2000 UP、 AMILITE GCK-12H和DIAPON K-SG)及其与苯氧乙醇不同搭配的抑菌率,来研究表面活性剂及其与防腐剂苯氧乙醇相互作用的抑菌效果。结果表明:3种表面活性剂对金黄色葡萄球菌和大肠菌杆菌都有一定的抑菌效果,且随质量分数增加抑菌作用逐渐增强;AMILITE GCK-12H和PLANTACARE 2000 UP等量复配时对金黄色葡萄球菌的抑菌效果较好,AMILITE GCK-12H和DIAPON K-SG等量复配时对大肠杆菌的抑菌效果较好;3种表面活性剂与苯氧乙醇搭配使用时,相互间无明显的拮抗作用,对两种细菌的抑菌率均高于97%。     

三桠苦提取物抑菌活性研究

目的：研究三桠苦提取物的抑菌活性。方法：以95%乙醇为溶剂,分别利用超声波提取和回流提取方法获得三桠苦提取物,利用滤纸片扩散法,分析两种提取方法的提取物对大肠杆菌、金黄色葡萄球菌、枯草杆菌和沙门氏菌的抑菌活性差异,确定抑菌活性较好的提取方法。利用该方法提取三桠苦得到提取物,采用溶剂萃取法,依次用石油醚、二氯甲烷、乙酸乙酯和正丁醇分别萃取三桠苦提取物,得到极性大小不同的萃取物,再次利用滤纸片扩散法,比较这些萃取物对四种菌的抑菌活性,确定三桠苦的抑菌活性部位,为三桠苦抑菌活性成分的深入研究提供参考。结果：回流提取法获得的样品抑菌活性优于超声提取法;石油醚、二氯甲烷萃取物表现出较好的抑菌活性,乙酸乙酯萃取物抑菌活性较差、正丁醇萃取物无抑菌活性。结论：三桠苦提取物具有抑菌活性,其二氯甲烷萃取物是具有较强抑菌作用的活性部位。     

板栗壳色素抑菌作用的研究

实验采用滤纸片扩散法和菌悬液涂布法研究不同质量浓度的板栗壳色素对黑曲霉菌(Aspergillus niger),白色念珠菌(Canidia albicans),大肠杆菌(Escherichia coli),枯草芽孢杆菌(Bacillus subtilis)菌株的抑菌性,比较两种方法的优缺点;同时测定温度对不同浓度板栗壳色素抑菌效果的影响,确定了每个菌株最低抑菌温度和最小板栗壳色素浓度的组合。实验结果表明,滤纸片扩散法板栗壳色素对于黑曲霉和白色念珠菌无明显抑菌作用,对大肠杆菌和枯草芽孢杆菌有明显抑制作用;菌悬液涂布法板栗壳色素表现出热稳定性,黑曲霉和白色念珠菌最小抑菌浓度和培养温度的组合为0.19%,25℃,对大肠杆菌和枯草芽孢杆菌最小抑菌浓度和培养温度的组合为0.375%,25℃或0.19%,100℃。菌悬液涂布法的抑菌效果较滤纸片扩散法好。     

穿破石抑菌活性初步研究

采用菌丝生长速率法测定了穿破石不同部位甲醇提取物对5种植物病原真菌的抑菌活性.结果表明:穿破石根提取物对梨锈病菌、稻瘟病菌、水稻纹枯病菌以及玉米炭疽病菌有很高的抑菌活性,质量浓度为10 g/L时,抑菌率分别为95.51%、99.39%、100.00%、86.11%;有效中浓度(EC50)分别为0.8031、0.9969、0.9812、0.9304 g/L.对柿角斑病菌的活性较低,质量浓度为10 g,L时,抑菌率为66.72%,EC50值为4.6539 g/L.茎叶提取物对上述5种病原菌的抑菌活性较低.采用固-液萃取法对穿破石抑菌活性成分进行了初步分离,结合活性跟踪,发现穿破石抑菌活性成分主要存在于乙酸乙酯萃取层中.     

D-氨基葡萄糖Schiff碱的制备及抑制真菌性能研究

以D-氨基葡萄糖盐酸盐与对甲氧基苯甲醛、对甲基苯甲醛反应合成了两种新的D-氨基葡萄糖席夫碱(对甲氧基苯甲醛缩D-氨基葡萄糖席夫碱、对甲基苯甲醛缩D-氨基葡萄糖席夫碱),收率分别为67.9%、74.6%。使用UV1、HNMR和IR对产物进行了结构表征。测试了两种席夫碱甲醇饱和溶液对香蕉炭疽病菌、芒果炭疽病菌、芒果蒂腐病菌、芒果焦腐病菌、番木瓜炭疽病菌等植物病原真菌的抑菌性能。结果表明,对甲氧基苯甲醛缩D-氨基葡萄糖席夫碱对香蕉炭疽病菌、芒果蒂腐病菌、芒果焦腐病菌具有很强的抑菌活性,对甲基苯甲醛缩D-氨基葡萄糖席夫碱对香蕉炭疽病菌、芒果炭疽病菌具有很强的抑菌活性。     

2-氨基苯并咪唑水杨醛席夫碱(SABI)钆(Ⅲ)配合物的合成、表征及抑菌活性的研究

以2-氨基苯并咪唑水杨醛席夫碱(SABI)为配体合成了新型金属钆(Ⅲ)的配合物,并通过元素分析、红外光谱、热重及摩尔电导率对其进行表征,确定配合物的组成为[Gd(HL)2(NO3)].H2O。席夫碱的配位原子是酚羟基的氧原子、席夫碱及咪唑环C=N中的氮原子。同时研究了配体及配合物的抑菌活性。     

含噻吩环新型席夫碱的合成研究

报道了2-乙酰噻吩(中间体)和两种新席夫碱的合成。以噻吩为原料与乙酸酐反应生成中间体。并用中间体分别和邻苯二胺及邻氨基对甲苯酚在无水乙醇中反应,得到了两种新型结构的席夫碱,并对中间体和产物的合成条件分别进行了探索性试验,确定了合成它们的各自优化条件;并对它们进行了UV、IR、1H NMR和13C NMR分析。     

Schiff碱及其配合物的应用进展

综述了Schiff碱及其配合物在催化剂领域、医药和农药领域、缓蚀剂领域、新材料领域、有机合成领域、分析化学领域的应用，为进一步研究、开发和利用Schiff碱奠定基础。     

Novel Poly(Styrene) Attached Schiff Bases for Uptake Mn(II) and Ni(II) Ions and as Antimicrobial Agent against Micrococcus luteus

In this study, three novel poly(styrene) attached Schiff bases (AMP–SB) were synthesized from condensation reaction of (aminomethyl)polystyrene with 2-hydroxy-5-bromobenzaldehyde, 2-hydroxy-5-methylbenzaldehyde and 2-hydroxy-5-chlorobenzaldehyde for uptake from solutions containing Mn(II) and Ni(II) ions and as antimicrobial agent against Micrococcus luteus. Furthermore coordination polymers were synthesized including Mn(II) and Ni(II) ions for understand to geometric structure of uptake ions. Polymers attached Schiff bases and coordination polymers were characterized by means of elemental analyses, magnetic measurements, FTIR, UV-GB, TG/DTA and ¹H-NMR. The Ni(II) and Mn(II) contents in the metal-containing polymer complexes were determined by the ICP-MS technique. Antibacterial activities of the polymers attached Schiff bases and their complexes were studied by the well-diffusion method against M. luteus (ATCC-9341).     

Preparation of schiff base adducts of phosphatidylcholine core aldehydes and aminophospholipids, amino acids, and myoglobin

We have prepared Schiff base adducts of the core aldehydes of phosphatidylcholine and aminophospholipids, free amino acids, and myoglobin. The Schiff bases of the ethanolamine and serine glycerophospholipids were obtained by reacting sn-1-palmitoyl(stearoyl)-2-[9-oxo]nonanoyl-glycerophosphocholine (PC-Ald) with a twofold excess of the aminophospholipid in chloroform/methanol 2∶1 (vol/vol) for 18 h at room temperature. The Schiff bases of the amino acids and myoglobin were obtained by reacting the aldehyde with an excess of isoleucine, valine, lysine, methyl ester lysine and myoglobin in aqueous methanol for 18 h at room temperature. Prior to isolation, the Schiff bases were reduced with sodium cyanoborohydride in methanol for 30 min at 4°C. The reaction products were characterized by normal-phase high-performance liquid chromatography and on-line mass spectrometry with electrospray ionization. The amino acids and aminophospholipids yielded single adducts. A double adduct was obtained for myoglobin, which theoretically could have accepted up to 23 PC-Ald groups. The yields of the products ranged from 12 to 44% for the aminophospholipids and from 15–57% for the amino acids, while the Schiff base of the myoglobin was estimated at 5% level. The new compounds are used as reference standards for the detection of high molecular weight Schiff bases in lipid extracts of natural products. Based on presentation at the AOCS Annual Meeting & Expo in Indianapolis, Indiana, April 28–May 1, 1996.     

生理活性氨基酸Schiff碱的合成与表征

用天冬酰胺分别与水杨醛、邻香草醛、2 ,4 二羟基苯甲醛在甲醇中反应 ,然后加入异丙醇析出产物的方法合成了三种氨基酸Schiff碱 ,产物收率分别为 84 3 %、81 2 %、76 7%。用元素分析、红外光谱、紫外光谱等手段对所合成的Schiff碱进行了表征。采用EPR技术对所合成的Schiff碱清除超氧阴离子自由基 (O2 ·)的性能进行了研究 ,结果表明 ,所合成的氨基酸Schiff碱质量浓度为 5kg/m3 时 ,对O2 ·的清除率分别达到 6 3 4%、6 2 .0 %、84 2 % ,具有明显的生理活性。     

Preparation and antibacterial activity of C2-benzaldehyde-C6-aniline double Schiff base derivatives of chitosan

C₂-benzaldehyde-C₆-aniline double Schiff base derivatives of chitosan were synthesized with positioning protection method for the first time. The structures and properties of the new synthesized products were characterized by Fourier transform infrared spectroscopy, ¹³C nuclear magnetic resonance, scanning electron microscope image, X-ray diffraction, and elemental analysis. The elemental analysis results indicated that the degrees of substitution of the products were from 39.6% to 48.2%. The synthesized compounds exhibited an excellent solubility in organic solvents. The antibacterial activities of all of the derivatives were tested in the experiment, and the results showed that the prepared chitosan derivatives had significantly improved antibacterial activity of chitosan and C₂-benzaldehyde Schiff bases of chitosan toward Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 35218). Antibacterial activity of the Schiff bases against E. coli differs from the substituent at the same position of different anilines and increases as the sequence aniline?>?p-toluidine?>?o-toluidine, and the antibacterial activity of the Schiff bases against S. aureus increases as the sequence p-toluidine?>?aniline?>?o-toluidine. The I_C50 of the C₂-benzaldehyde-C₆-aniline double Schiff base derivatives of chitosan against E. coli and S. aureus is 0.0035 and 0.0031?mg?L^?1, respectively, much lower than that of chitosan (0.0064?mg?L^?1) and C₂-benzaldehyde Schiff bases of chitosan (0.0054?mg?L^?1). The cytotoxicity test showed that compared with chitosan and C₂-benzaldehyde Schiff bases of chitosan, the prepared chitosan derivatives had lower cytotoxicity against SCG-7901. This paper provided a new method for the synthesis of Schiff bases of chitosan, which was enlightening.     

超声波促进新型席夫碱类化合物的水相合成

在超声波促进下以聚乙二醇(PEG 6000)为相转移催化剂,在水相中合成了8个席夫碱类化合物。采用1HNMR、13CNMR、IR、MS(ESI)和X-射线衍射对其结构进行了表征,结果与目标化合物一致。采用相转移催化剂水相超声波合成,与传统的加热反应或有机溶剂相超声波合成比较,水相超声波合成不但可以避免使用酸碱催化剂和乙醇等挥发性溶剂,而且使得反应时间从数小时缩短至0.5 h左右,产率高达76%~92%。经查证,其中6个席夫碱类化合物是未见文献报道的新化合物。     

丹皮酚缩脂肪胺Schiff碱的合成及生物活性研究

以丹皮酚和脂肪胺为主要原料,合成9种Schiff碱化合物,并对标题化合物进行表征。利用细菌作为生物指示,采用琼脂扩散法测定了目标产物的抑菌性能;通过DPPH.分析法,测定了目标产物的抗氧化性。结果表明:目标产物对所受试菌种都有一定的抑制作用,但其清除自由基能力比较弱。