共查询到19条相似文献,搜索用时 671 毫秒
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目的 建立一种简单、准确的卵磷脂测定方法。方法 采用KOH溶液将磷脂酰胆碱中的胆碱游离, 加雷氏盐反应后, 在波长525 nm处, 通过分光光度法测定胆碱的量。结果 该方法在0.075~0.225 mmol 范围内线性关系良好, 线性方程为Y = 3.6457X+0.026, R2 = 0.9987, 方法的回收率在95%~103%之间, 相对标准偏差小于1.0%, 待测液在2.5 h之内稳定, 能够满足检测要求。结论 本方法具有操作简单、准确性高、稳定性好、抗干扰性强的优点, 可以用于卵磷脂产品中磷脂酰胆碱含量的测定。 相似文献
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对鸡蛋中的卵磷脂含量进行测定。采用干法灰化法、湿式消解法和微波消解3种方式进行消解,运用紫外分光光度法进行测定鸡蛋中的卵磷脂含量。结果:微波消解前处理具有简单快速、试剂用量少、准确度高等优点,优于干化消解法和湿式消解法。 相似文献
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鸡蛋黄中卵磷脂的分离及分析方法研究 总被引:1,自引:0,他引:1
采用单一溶剂和混合溶剂提取鸡蛋黄中的卵磷脂,以期获得较经济、较环保的提取方法,并建立鸡蛋黄卵磷脂含量快速测定方法。分别利用单一溶剂和混合溶剂抽提法提取鸡蛋黄中的卵磷脂;将粗提蛋黄脂肪经高氯酸-硝酸消化液消化后与钼酸铵反应,于分光光度计400 nm处进行比色测定磷含量。最后测定了回收率。结果表明,混合溶剂在提取率和纯度上均优于单一溶剂;磷标准曲线在0~25μg/mL浓度内呈线性相关。回收率为84.3%~93.4%,精密度为1.93%~2.90%。该方法符合食品卫生分析要求,可用于鸡蛋黄卵磷脂含量分析。得到了提取卵磷脂较经济、环保的方法,对扩大卵磷脂生产规模有一定的参考价值。 相似文献
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磷脂最初是在鸡蛋黄中被发现的,故称之为“卵磷脂”。后来,人们惊异地发现大豆是磷脂更为丰富的来源,且容易获取,价格低廉。现在,无论是工业用作添加剂的卵磷脂,还是人们用作营养品、保健品的卵磷脂,都是提取自大豆的大豆磷脂,只是仍沿袭了卵磷脂的称呼。 相似文献
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为探讨从蛋黄中提取蛋黄卵磷脂的方法。试验以蛋黄为原料,采用有机溶剂和酶解法从蛋黄中提取卵磷脂粗品,然后分别经柱硅胶层析和金属离子沉淀法纯化获得卵磷脂精品,通过测定卵磷脂的含磷量,判断其纯度高低。结果显示:采用有机溶剂法获得蛋黄卵磷脂粗品,然后经柱硅胶层析纯化可获得高纯度蛋黄卵磷脂精品,总得率为8.38%,卵磷脂的含磷量为15.33%。是一种高效、经济的蛋黄卵磷脂提取方法。 相似文献
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蛋白质与卵磷脂间发生的相互作用可以增强蛋白质功能特性,且二者形成的复合体系不仅能够作为递送系统传递生物活性物质、药物等以达到在胃肠道中的缓释效果,提高包埋物质的装载率及体外释放度,还能够实际应用于食品工业中提高我国传统蛋白基产品的质量及拓宽种类,如蛋白粉、婴儿配方乳以及运动营养食品等。本文综述了玉米醇溶蛋白、大豆蛋白、卵磷脂的理化性质和功能特性,蛋白-卵磷脂二者间的相互作用对蛋白的改善,蛋白-卵磷脂复合纳米颗粒、乳液及凝胶的特性,以及复合体系在食品工业、医药等领域作为递送系统和提高食品质量的应用,为改善单一蛋白体系的稳定性及提高其应用价值提供参考价值依据,对促进我国蛋白基食品产业高质量发展具有重要意义。 相似文献
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Moustapha Oke Jissy K. Jacob Gopinadhan Paliyath 《Food research international (Ottawa, Ont.)》2010,43(1):232-240
Phosphatidylcholine is a major component of biological membranes and is an important mediator of physiological functions in mammals. In addition to biosynthetic routes of availability, phosphatidylcholine is also derived from the diet. The levels of phosphatidylcholine are quite low in fruits and fruit products. As the fruits ripen, the phospholipids are degraded into neutral lipids causing a drastic decline in phospholipid content. In the present study, we have evaluated the effects of external supplementation of soy lecithin on physico-chemical properties of tomato juice and sauce, as well as grape juice. The levels of protein, soluble solids and ash content were significantly enhanced by the addition of lecithin. The bulk viscosity of tomato juice and the sauce preparation was also enhanced by addition of lecithin. The yellow color values of the juice and sauce preparations were enhanced by lecithin. In grape juice preparations, the addition of lecithin did not cause any negative effects, but enhanced the solids content of the juice. Juice preparations with added lecithin were more stable than the controls. Lecithin may form complexes with pectin, lipids and other carbohydrates, affecting the food property and stability by various mechanisms. 相似文献
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Olivera Koprivnjak Dubravka Škevin Srećko Valić Valerija Majetić Sandra Petričević Ivica Ljubenkov 《Food chemistry》2008
Virgin olive oil (VOO) enriched with phospholipids (soy lecithin) up to the levels present in seed oils (from 2.5 to 10.0 g/kg) was studied as a potential functional food. Lecithin addition slightly increased the concentration of tocopherols and considerably increased K270 values. In the fatty acid composition, an increase of linoleic and a slight decrease of oleic acid were observed, as the decrease of monounsaturated/polyunsaturated fatty acid ratio. The radical-scavenging activity was evaluated by two methods: electron spin resonance spectroscopy using galvinoxyl free radical and VIS spectroscopy measurement of the disappearance of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical. Results indicate that lecithin addition retards the scavenging activity of VOO that is ascribed to the bipolar character of lecithin and its ability to entrap hydrophilic antioxidants. The effect of lecithin addition on the oxidative stability of VOO was evaluated by the Rancimat method, and a positive linear correlation (r = 0.9849) with induction time was found. 相似文献
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为了得到高质量的大豆磷脂,用于制取磷脂的毛油在水化之前应进行过滤除杂,蒸汽、水是水化脱胶的理想用水,合适的水量为相当于毛油中磷脂含量,很多脱胶剂对磷脂有不良作用,但乙酸酐除外,乙酸酐在水化时可与磷脂酰胆胺形成乙酰化磷脂,在水化脱胶时加磷酸使水化油脚在浓缩时磷脂焦化变黑,加无机盐影响磷脂的质量和性能,在实际离心分离损伤中,应注意离心压力差而不同被压。 相似文献
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为准确快速地对卵磷脂保健食品中有效成分进行定量分析 ,我们建立了卵磷脂保健食品中磷脂主要组分磷脂酰胆碱 (PC)、磷脂酰乙醇胺 (PE)的高效液相色谱测定方法。方法的日间精密度为 5 4 %~ 9 0 %;PE、PC的加标回收率分别为 83 0 %~ 10 5 6 %和 81 3%~ 86 0 %,均可满足卫生分析的要求。对 5种卵磷脂保健食品中的PC、PE的含量进行了测定 ,PC、PE的含量分别为15 2 %~ 2 4 2 %和 3 1%~ 6 1%。方法的样品前处理方法简单 ,且检测灵敏度高 ,分析速度快 ,适于在国内推广使用 相似文献