啤酒废酸母残余内源蛋白酶活力的测定及其对酵母自溶的影响

采用超声波破碎法对啤酒废酵母残余的内源蛋白酶进行提取,并以酪蛋白为底物测定内源蛋白酶的活力。通过单因素实验和正交实验,选出了超声波破碎的最佳工艺条件。实验表明:在功率600W,工作时间20min,占空比0.25,反应温度60℃时,超声波对啤酒废酵母的破碎效果最好,此破碎条件下残余内源蛋白酶的活力为1.20×106U/kg(干酵母)。同时,研究了残余内源蛋白酶活力对酵母自溶产物的氨基氮和固形物得率的影响,结果显示它与后两者之间存在显著的相关性。     

利用有活力和无活力的啤酒废酵母生产酵母抽提物

以两种形态的啤酒废酵母(有活力的和无活力的)为原料,制备酵母抽提物。实验表明,采用自溶法处理啤酒废酵母液(有活力的),最佳作用条件是在温度45℃、pH 5.5下,自溶28 h,所得酵母抽提液的氨基氮含量为5.16 g/L,氨基氮得率为6.77%,产品得率为69.01%;酶采用酶水解法添加木瓜蛋白处理啤酒废酵母粉(无活力的),最佳作用条件是在木瓜蛋白酶添加量2.5%(以酵母干重计)、温度55℃p、H 4.5下,酶水解时间18 h,所得酵母抽提液的氨基氮含量为3.35 g/L,氨基氮得率为4.31%,产品得率为76.66%。     

啤酒废酵母综合破壁法提取酵母味素技术

本文研究了高压均质、酶法溶胞、冻融法三种细胞壁破碎法对啤酒废酵母抽提物（酵母味素）的得率、蛋白质的转化率及氨基氮溶出率的影响。实验结果,在适宜的自溶条件下,利用综合破壁法可使酵母抽提物中上述三项指标显著提高。     

啤酒废酵母综合破壁法提取酵母味素技术

研究了高压均质、酶法溶胞、冻融法三种细胞壁破碎法对啤酒废酵母抽提物(酵母味素)的得率、蛋白质的转化率及氨基氮溶出率的影响.实验结果,在适宜的自溶条件下,利用综合破壁法可使酵母抽提物中上述三项指标显著提高.     

啤酒废酵母酶法制备生物活性肽的工艺研究

以啤酒废酵母为原料,经菠萝蛋白酶酶解、分离、纯化、干燥等工序制得啤酒废酵母活性肽.试验通过单因素及响应面试验分析确定了菠萝蛋白酶对废酵母的最佳酶解条件:酶解温度40.61℃,pH6.58,酶用量2.62%(w/w).酶解后多肽得率可达到50.97%(w/w).     

利用啤酒废酵母制备富锌酵母

以啤酒废酵母为原料制备富锌酵母.对制备的工艺条件进行了优化.确定最佳的工艺条件条件为:Zn2+的浓度为500μg/mL;培养pH值为4.5:振荡培养时间为10h;啤酒酵母投加量为50g/50mL培养基.通过红外分析对空白废酵母和富锌废酵母进行比较,分析了酵母富集锌前后吸收峰的变化.     

促进提取啤酒废酵母内溶物的实验研究

研究了高压均质、酶法溶胞、冻融法三种细胞壁破碎法对啤酒废酵母抽提物的得率、蛋白质的转化率及氨基氮含量的影响。实验结果表明,在适宜的自溶条件下,利用综合破壁法可使酵母抽提物的得率、蛋白质的转化率及氨基氮的含量显著提高。     

啤酒废酵母泥蛋白质营养价值的评价

本文评价了啤酒废酵母蛋白质的营养价值，并对实验动物进行了相关测定，动物实验表明，啤酒废酵母蛋白质的营养价值是比较优良的。因此，啤酒废酵母是一项值得开发，能变放心为宝的优质蛋白食物资源，对于改善我国以植物性食品为主的膳食结构，具有一定的营养和经济效益。     

啤酒废酵母生产酵母浸粉及其应用的研究进展

全面系统阐述啤酒废酵母生产酵母浸粉及应用的研究进展.通过分析,指出原料的预处理、破壁方法和自溶技术是酵母浸粉生产工艺的核心.预处理一般通过稀释、过筛除杂和沉降分离完成.破壁方法有物理法、化学法、酶处理法等,综合破壁法已经成为研究的焦点.自溶的强化可以通过添加化学物质激活内源酶和添加外源酶实现.某些微生物的培养可以使用国产酵母浸粉替代部分进口产品,节约生产成本,达到其最优化使用.酵母浸粉的生产不仅具有可观经济效益,而且促进了企业清洁生产,社会效益也相当可观,所以利用啤酒废酵母生产酵母粉将成为酵母浸粉生产行业的趋势.     

啤酒废酵母综合破壁法提取酵母味素技术

研究了高压均质、酶法溶胞、温差破壁3种细胞壁破碎法对啤酒废酵母抽提物(酵母味素)的得率、蛋白质的转化率及氨基氮溶出率的影响.结果表明,在适宜的自溶条件下,利用综合破壁法可使酵母抽提物中酵母味素得率、蛋白质的转化率及氨基氮溶出率显著提高.     

利用啤酒废酵母制备酵母抽提物的试验研究

以啤酒生产的废弃酵母为原料,制备酵母抽提物.经各种单酶及各单酶间的复合试验,筛选出碱性蛋白酶与胰蛋白酶进行复合酶解,在料水比1:4、复合酶添加量0.4%(以干酵母粉计,2种酶添加量各为0.2%)、酶解温度45℃、酶解pH值8.5、酶解时间18d～20d的优化工艺条件下,酵母抽提物α-氨基酸态氮的含为4587mg/L.应用蛋白酶复合酶解制备酵母抽提物具有收率高、时间短、设备简单、操作方便、成本低、利于推广应用等优点.     

Current awareness on yeast

In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (5 weeks journals ‐ search completed 2nd. Oct. 2002)     

Current awareness on yeast

In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 24th. July 2002)     

Current awareness on yeast

In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 10th. Nov. 2004)