光电比浊法测定镁离子的分析方法研究

本文研究了应用光电比浊法测定镁离子的最佳测量条件，建立了测定镁离子的新方法。该方法已用于测定盐及盐化工产品镁的分析，获得了满意结果。与其他方法相比，具有操作简便快速，精确度高的特点。     

准确测定己酸菌液中己酸含量的方法

在生产实践中发现，使用721型分光光度计——比色法测定己酸菌液中己酸含量的方法不够准确。通过采用不同方法测定对比，确定了通过蒸馏己酸菌液，然后用色谱法测定其中己酸的含量的方法。该法分析准确度高，避免了比色法的误差。(陶然)     

双波长分光光度法测定组合浆料组分含量的研究

对同时测定组合浆料中ＰＶＡ和淀粉磷酸酯含量的双波长分光光度法进行了研究，介绍了双波发光光度法测定原理和方法，并对测试结果进行了分析，讨论，实验证明，用该方法同时测定双组分含量，即简单又准确。     

IDF奶粉酸度测定标准的验证实验及对国标方法的修改意见

报告了奶粉酸度测定的ＩＤＦ基准法，ＩＤＦ常规法和国家标准方法比较实验结果，对这３种方法进行了评价，并给出了乳粉酸度测定的国家标准方法的修改意见。     

食盐中微量碘酸钾的测定——分光光度法

本文研究了用分光光度法测定食盐中微量碘酸钾，探讨了最佳测定条件，用于加碘盐及模拟盐中微量碘酸钾的测定，结果满意，回收率高，操作快捷，方法简单。     

微波消解-原子荧光光谱法测定饮料中镉

目的：建立原子荧光光谱法测定果汁饮料中镉的检测方法。方法：采用微波消解法消解果汁饮料样品后，用原子荧光光谱法测定其中的痕量镉的含量，对测定条件进行了优化，并对测定方法进行了方法学考察。结果：镉的校准曲线的相关系数r=0．9995，线形范围为0-60．0μg／L，检出线为0．0472μg／L，回收率为94．9％-100．5％，RSD为5％。结论：该方法快速、简便、准确且灵敏度高，为果汁饮料中镉含量的测定提供了较好的方法，用本方法测定标准样品达到满意效果。     

谈谈对甲醛法成曲酶活力测定的一点看法

本文提出了用甲醛测定成曲酶活力时，空白测定的新方法，并与现行方法进行比较。提出了改进方法和意见。     

测定脂肪酸值操作方法的改进

介绍了测定粮食脂肪酸值的两种操作方法，一是国家标准方法，二是改进后操作方法，并对这两种方法的测定结果进行比较，得出减少试样用量及浸出溶剂用量，其测定结果同样准确、可靠。     

回潮率测双组分混纺产品混纺比的评判研究

本文分析了各种测定混纺材料混纺比的方法，介绍了回潮率测定双组分混纺产品混纺比的原理，并对此方法作了相应的评判研究。     

用分光光度计测定填料留着率

在造纸过程中，填料留着率传统的测定方法是通过灼烧后测定纸浆中灰份的方法进行。采用该方法测定填料留着率，测定操作复杂，测定所需时间长且精确性差，近几年来，随着制浆造纸实验手段的不断进步，造纸工作者已开始探索新的测定造纸过程中填料留着率的更简捷、准确的方法。我们知道，当入射光的强度与液层厚度一定时，溶液吸光度与溶液的浓度成正比，对于悬浮液也是如此，因此，可以通过分光光度计测定造纸过程中白水的吸光度的方法测定填料的留着率，本文以蔗渣浆为原料对此问题进行了研究。研究方法及结果对于其它浆种均具有一定的参考价值     

高碳水化合物配方早餐对健康者餐后血糖影响

目的:研究高碳水化合物配方早餐(HCD)对健康志愿者餐后血糖的影响。方法 :10名健康志愿者为研究对象,先后摄人A、B、C、D、E套早餐,测量空腹、餐后0.5h、1h、2h的血糖值,计算血糖曲线下面积;问卷调查患者有无饥饿感。结果 :B餐血糖曲线下面积(SAUC)(19.72±1.66)明显高于A餐(16.03±1.25)(P<0.01)与C餐(16.81±2.23)(P<0.01),没有饥饿感的人数明显多于B餐和C餐。结论:合理配制的高碳水化合物配方早餐对健康志愿者餐后血糖调控有效。     

Human pancreatic glucokinase (GlkB) complements the glucose signalling defect of Saccharomyces cerevisiae hxk2 mutants

Human pancreatic glucokinase (GlkB, hexokinase IV) has been expressed in Saccharomyces cerevisiae. The recombinant protein showed similar enzyme kinetics to those described for the original enzyme. When expressed in hxk2 yeast mutants, GlkB complemented both the glucose induction and the glucose repression defects present in the mutant. It was also functional in regulating the activity of the Snf1 kinase complex in response to glucose, participating in the regulation of the Reg1/Glc7 phosphatase complex, as its yeast counterpart.     

用葡萄糖氧化酶法降低马铃薯颗粒全粉还原糖

观察了葡萄糖氧化酶 (GOD)对降低马铃薯颗粒全粉中葡萄糖及还原糖的作用。结果表明 ,当控制马铃薯颗粒全粉的水分在 5 0 %～ 60 % ,酸度为 3 5 ,温度为 3 9℃时 ,GOD的酶解降糖速度最快。在此条件下用GOD(5 0 0U/kg产品 )反应 1 80min ,可以使原料中的葡萄糖减少 80 %～ 90 % ,还原糖减低 3 0 %以上。     

Glucose metabolism in lactating cows in response to isoenergetic infusions of propionic acid or duodenal glucose

A bibliographical study showed that increasing supplies of glucogenic nutrients lead to a curvilinear increase in milk and protein yield. Increased post-hepatic glucose availability may be involved in the increase in milk yield. In the present experiment, 5 dairy cows were arranged in a 5 x 5 Latin square design to compare the respective effects of 2 amounts of either duodenal glucose or ruminal propionic acid (C3) on glucose metabolism. Treatment consisted of a grass silage-based diet supplemented with glucogenic nutrients infused into the rumen as a mixture of volatile fatty acids (control) or C3 (6.5 and 13 mol/d) or as glucose (3.4 and 6.9 mol/d) infused into the duodenum. Treatments were isoenergetic and isonitrogenous and contained 100 and 115% of energy and protein requirements, respectively, according to the Institut National de la Recherche Agronomique. Glucose appearance rate (Ra) tended to increase with the level of infusions of both glucogenic materials and with the high dose of duodenal glucose. Plasma insulin-like growth factor-I (IGF-I) concentration increased with the infusion of glucogenic materials compared with the control and was significantly higher with glucose than with C3 treatments. This experiment did not indicate whether the increased Ra was the key mechanism to increased milk yield because milk yield only tended to increase and the standard error for Ra was high. With the high dose of glucose infused into the duodenum, the Ra increase was greater than the increased lactose production in milk. Because of that connection, IGF-I may also be involved by favoring the glucose utilization by the mammary gland.     

预处理和贮藏条件对脱水甘蓝表面返霜的影响

添加葡萄糖的脱水甘蓝在贮藏过程中葡萄糖极易从甘蓝内部迁移到表面．实验结果表明，渗糖处理时添加一定量的乳糖和高麦芽糖浆代替葡萄糖，对延长脱水甘蓝的返霜期有良好的效果，高麦芽糖浆对延长脱水甘蓝返霜的效果明显好于乳糖．实验还研究了热烫时间、贮藏环境的相对湿度对葡萄糖迁移影响的规律．热烫90s、贮藏在较低的环境湿度下均有利于延长脱水甘蓝的返霜天数．     

红枣多糖对小鼠血糖及血清胰岛素水平影响的初步研究

研究红枣多糖对正常小鼠血糖及糖耐量的影响,结果表明红枣多糖对正常小鼠血糖没有影响,对正常小鼠糖耐量有改善作用。为研究红枣多糖对四氧嘧啶、肾上腺素诱导的糖尿病小鼠血糖及血清胰岛素水平的影响,以200mg/kg剂量腹腔注射四氧嘧啶建立四氧嘧啶糖尿病动物模型,以0.03mg/kg剂量腹腔注射肾上腺素建立肾上腺素高血糖动物模型。对造模成功的小鼠随机分为模型对照组、红枣多糖大(0.8g/kg)、中(0.4g/kg)、小(0.2g/kg)剂量组和阳性药物组,以灌胃给药。对两种类型小鼠的处理均设立正常对照组,给予等体积的水。灌胃14d后测定小鼠空腹的血糖、胰岛素,结果表明红枣多糖对肾上腺素引起的急性血糖升高无明显作用,对四氧嘧啶诱导的糖尿病小鼠其大剂量显示出一定的降糖作用,并使得血清胰岛素水平有所升高。     

高纯度低聚果糖生产技术的探讨

低聚果糖是一种功能性低聚糖，用于特殊目的的低聚果糖必须具有很高的纯度。本文探讨了高纯度低聚果糖的生产技术     

Duodenal glucose increases glucose fluxes and lactose synthesis in grass silage-fed dairy cows

The effect of intestinal glucose supply on whole body rate of glucose appearance (WBGRa) and mammary utilization of glucose was studied in four lactating dairy cows. Glucose (0, 443, 963 and 2398 g/d) was continuously infused in the duodenum over 14-d periods using a Latin square design. A grass silage-based diet was formulated so that treatments were isoenergetic and isonitrogenous and contained 100 and 110% of energy and protein requirements according to INRA (1989). The WBGRa was measured by the [6,6-(2)H2]glucose dilution technique, and mammary glucose balance by arteriovenous differences and blood flow measurements. Duodenal glucose infusion increased arterial glucose concentrations linearly, whereas arterial concentrations of insulin, growth hormone, and glucagon were not changed. The WBGRa increased linearly with increasing glucose loads. The increase represented 42% of the intestinal glucose supplement. Mammary blood flow dramatically increased (up to 45%) and was associated with a significant increase of arterial insulin-like growth factor-1 concentrations. Mammary gland rate of glucose disappearance ([6,6-(2)H2]glucose measurement) increased linearly, whereas net mammary balance of glucose, lactose, and milk yields increased quadratically. Net mammary balance of glucose accounted for 60% of WBGRa, except for the greatest dose (47.6%). The decrease in milk yield with 2398 g/d of glucose may be explained by an imbalance in intracellular intermediate concentrations. The milk ratio of glucose-1-phosphate to glucose-6-phosphate decreased significantly at the greatest infusion of glucose. In conclusion, exogenous glucose supply to a grass silage-based diet increased WBGRa, mammary utilization of glucose and milk synthesis.     

盐酸精氨酸葡萄糖注射液中葡萄糖含量的测定

目的建立测定盐酸精氨酸葡萄糖注射液中葡萄糖含量的方法。方法选择适宜的pH值,然后通过考察该pH值下溶液旋光度与盐酸精氨酸和葡萄糖浓度的线性关系并测定盐酸精氨酸的比旋度,建立旋光法测定葡萄糖含量的计算公式。结果测定pH值为4.2。在此pH值下,盐酸精氨酸和葡萄糖混合溶液的旋光度与盐酸精氨酸浓度和葡萄糖浓度的复相关系数为0.9999,线性范围分别为2.3～3.0g/100ml和1.8～3.2g/100ml;测得盐酸精氨酸的比旋度为11.54。结论盐酸精氨酸和葡萄糖的旋光度具有加和性,从测得的旋光度中扣除盐酸精氨酸的旋光度,即是葡萄糖的旋光度,葡萄糖的含量可按公式c=2.0852(α-0.1154c1)计算。