如何利用"分光光度计检定装置"解决"酶标分析仪"的检定问题

随着生物工程的发展，以及卫生防疫等部门对高难病症预防检测工作的加强，“酶标分析仪”等仪器显得越来越重要。但“酶标分析仪”的检定工作进行的并不顺利，原因是检定“酶标分析仪”的标准器没有批量生产单位。下面介绍一下利用“分光光度计检定装置”进行“酶标分析仪”的检定方法。检定分光光度计用的“光谱中性玻璃滤光片”是每个计量检测部门必备的，该滤光片每年送检一次。那么在下次送检时，您可以要求检定单位除了在440nm、546nm、635nm波长处标定透射比和吸光度值外，对30%和10%两片中性滤光片同时在405nm、492nm、620nm波长处定…     

可见分光光度计波长示值误差测量不确定度分析

文章以可见分光光度计主标准装置之一的干涉滤光片对上海第三分析仪器厂生产的721型分光光度计检定为例,进行波长示值误差测量不确定度评定。     

标准滤光片的应用

分光光度计是一种广泛使用的光学分析仪器，为保证这种分析仪器的准确可靠，国家技术监督局将其列为强检仪器，并先后颁布了各类分光光度计的检定规程。为了配合检定规程的实施，近年来军队各医学计量机构都相继配备了标准滤光片用于各类分光光度计的检定。但是，由于分光光度计的种类繁多，其适用的检定规程及标准滤光片也不尽相同，因此在具体检定过程中，计量人员往往感到难以开展或不能完成某台机器检定的全过程。这里就几种主要标准滤光片在分光光度计检定中的应用方法作一介绍，供同行参考。1干涉德光片干涉滤光片是用来获得近似单色…     

金属镀膜型紫外可见光谱中性滤光片

金属镀膜型紫外可见光谱中性滤光片具有光谱范围宽,覆盖紫外、可见光谱区及光谱中性好的优点。 这种滤光片适用于下列标准: ZBN 33008—89可见分光光度计1994年1月1日实施; JB/T 6777—93紫外可见分光光度计1994年1月1日实施(替代ZBY 196—83); JB/T 6778—93紫外可见近红外分光光度计     

分光光度计特定波长和透射比示值误差测量结果的不确定度分析及评定

一、概述按JJG178-2007《紫外、可见、近红外分光光度计》检定规程和JJF1059.1-2012《测量不确定度评定与表示》要求,使用本单位的计量标准器测量滤光片特定波长区域中的特征波长,中性滤光片在特定波长下的透射比值,各连续测量6次,对其测量的结果进行不确定度分析和评定。     

JJG681-90中杂散辐射断档区间弥补检定的探讨

文章以实验数据为依据，说明了在色散型红外分光光度计的检定工作中，国家计量检定规程《色散型红外分光光度计JJG681－90》中杂散辐射滤光片波数覆盖面的断档现象，有些是可以弥补的。适当地延长波数覆盖面，有的波数段的检测就可以用相邻波数段的标准器来囊括，可省一台标准器，这就使建标和检定工作更简要更合理。     

分光光度计紫外区透射比检定两种方法的比较

本文依据JJG 178-2007《紫外可见近红外分光光度计》,分析了在紫外光区段用紫外分光光度计标准溶液和紫外光区透射比滤光片两种检定方法,并总结了两种方法优缺点。     

色散型红外分光光度计检定标准器杂散辐射标准滤光片的两个问题

本文以实验数据为依据，说明了在色散型红外分光光度计的检定中，杂散辐射滤光片的波数覆盖面的断档现象有些是可以弥补的，甚至有的波段可以用别的标准片来代替。这就使建标和检定工作更简便更经济合理。     

紫外、可见分光光度计检定或校准结果的测量不确定度评定

正一、波长示值误差测量结果的不确定度评定1.概述(1)测量依据JJG178-2007《紫外、可见、远红外分光光度计检定规程》。(2)测量环境条件温度10℃～35℃,相对湿度不大于85%。(3)测量标准氧化钬滤光片不确定度U=0.3nm,包含因子k=2。(4)被测对象用TU-1810双光束紫外-可见分光光度计对标准氧化钬滤光片测试。(5)测量方法在规定的条件下,用双光束紫外-可见分光光     

湖北省荆门市计量所4项计量标准通过考核

日前．湖北省计量测试研究院专家考核组对荆门市计量所申请新建的气相色谱仪检定装置、液相色谱仪检定装置、原子吸收分光光度计检定装置、二等铂电阻温度计标准装置等4项全市最高计量标准进行现场考核,同时对一等铂铑10一铂热电偶标准装置进行计量标准复查。现场试验结果符合检定规程的要求。     

Estimation of Parameters of Universal Distribution of Moments of Failure of Products

A method is proposed for estimating the parameters of a mixture of exponential and Weibull distributions for which the accuracy of preliminary estimates obtained by graphical analysis is refined in accordance with the criterion of maximum likelihood. The efficiency of the proposed method is supported by the results of statistical modeling.     

Evaluation of optical properties of highly scattering media by moments of distributions of times of flight of photons

A novel method for the determination of the optical properties of tissue from time-domain measurements is presented. The data analysis is based on the evaluation of the first moment and the second centralized moment, i.e., the mean time of flight and the variance of the measured distribution of times of flight (DTOF) of photons injected by short (picosecond) laser pulses. Analytical expressions are derived for calculation of absorption and of reduced scattering coefficients from these moments by application of diffusion theory for infinite and semi-infinite homogeneous media. The proposed method was tested on experimental data obtained with phantoms, and results for absorption and reduced scattering coefficients obtained by the proposed method are compared with those obtained by fitting of the same data with analytical solutions of the diffusion equation. Furthermore, the accuracy of the moment analysis was investigated for a range of integration limits of the DTOF. The moment analysis may serve as a comparatively fast method for evaluating optical properties with sufficient accuracy and can be used, e.g., for on-line monitoring of optical properties of biological tissue.     

Estimation of potentialities of methods of summation of direct measurement errors

Conventional methods for summation of direct measurement errors are considered. Conditions under which these methods provide consistent results are stated. It is proposed to utilize for characteristic of the total error, its interval estimator or the half-sum of the value obtained by adding up its arithmetic and geometric random errors.Translated from Izmeritel'naya Tekhnika, No. 4, pp. 10–12, April, 1995.     

空压机轴承箱蜗壳温度探头油封改造

由于空压机进气侧轴承箱蜗壳温度探头密封效果不好,使空压机及其后续管道受到油污染。后将温度探头的O形圈密封改为骨架油封,杜绝了轴承箱漏油问题。文章简介空压机的结构和技术参数,详细介绍了骨架油封的制作和优点。     

Characteristics of mechanical properties of materials in studies of conditions of attainment of marginal states

It is shown that conditions of attainment of marginal states in the material of structural components are formulated on the basis of analysis of force and deformation criteria of formation and development of destruction. The parameters of these criteria are data on types of structural materials, standard and special characteristics of their mechanical properties, structural forms of considered elements of the equipment, and their operating stress loading. In this case, the construction of a system of state equations for describing interrelation of current damage, external actions, and responses to them is based on the concept of calculating variations in properties of materials at all stages of the design life cycle.     

Determination of parameters of microstructural inhomogeneity of deformation of metals by the method of modeling

The article suggests a method of evaluating the microstructural inhomogeneity of deformation of metals by modeling. The method includes the measurement of deformations of metal according to meshes of a dividing grid with small base and the calculation of the distribution parameters of microdeformations in model representation. It is shown that the modeling method makes it possible objectively to evaluate the microinhomogeneity of deformation of metals independently of the chosen mesh size of the dividing grid. New structural characteristics are introduced: the base and wave of variation expressing the degree of crumbling or amalgamation of metal grains by inhomogeneity of deformation.Translated from Problemy Prochnosti, No. 7, pp. 40–44, July, 1991.     

Analysis of the rate of propagation of the wave of change of boiling modes

An approximate analytical expression is derived for the velocity of the front of the wave of change of boiling modes on a rod and on a plate. The impact of the Thomson effect and of the heater orientation in the gravity field is included. Adequate agreement is demonstrated between the derived relations and experimental data.     

水三相点复现的不确定度评定

水三相点是开尔文热力学温度的唯一基准点，也是ITS-90国际温标重要的定义固定点。因此，水三相点不确定度分析对整个温标的建立、温度量值传递起着至关重要的作用。近3年来，中国计量科学研究院研制出一系列高质量的水三相点容器，加强了水三相点的研究，为不确定度的分析提供了更为可靠的实验依据。同时，不确定度的分析也是客观评价新研制容器性能的一个重要指标。因此，根据实验结果对新研制容器所复现的水三相点进行了不确定度评定。评定结果表明，其扩展不确定度为0．16mK(k=2．69，P=0．99)。