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以南五味子总木脂素、多糖为指标,研究南五味子有效成分的最优提取工艺条件及其在乙醇相和水相中的分布状态,采用紫外分光光度法和苯酚-硫酸法进行检测。结果表明:总木脂素最优提取工艺为乙醇浓度70%、料液比为1:9、提取3次、每次2 h,得率可达9.76 mg/g生药量;总多糖最优提取工艺为70%乙醇、料液比为1:7、提取3次、每次1.5 h,得率可达11.32 mg/g生药量。结果还表明,提取物经分离后在乙醇相中总木脂素和多糖含量均明显大于水相中含量。乙醇相中,总木脂素与多糖含量相当;水相中,多糖含量高于总木脂素量。 相似文献
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为了获得川楝素最佳提取方法,以川楝果实为材料,采用高效液相色谱法检测,通过正交实验,分别研究溶剂浸提法、索氏提取法和超声波法提取川楝素的最佳提取条件。结果表明:溶剂浸提法的最佳提取工艺条件为:乙醇浓度70%,液料比8:1(mL/g),提取时间20min,提取次数3次,川楝素得率0.482%;索氏提取法提取川楝素的最佳工艺条件为:乙醇浓度70%,液料比15:1,提取时间4h,川楝素得率0.599%;超声波法提取川楝素的最佳工艺条件为:乙醇浓度80%,液料比12:1(mL/g),提取时间35min,超声功率450W,川楝素得率0.580%。三种提取方法进行比较,索氏提取法川楝素得率最高,为川楝素的最佳提取方法。 相似文献
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利用单因素试验和正交试验研究了以乙醇作为溶剂对荷梗中黄酮类化合物的提取工艺以及对不同季节荷梗中黄酮类化合物含量进行研究。确定最佳工艺条件为:提取温度70℃、乙醇浓度75%、料液比为1:30、时间3h.,黄酮类化合物的得率为0.582%。9月份的含量最高。 相似文献
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This study examined the application of pressurized low polarity water (PLPW) extraction of lignans, proteins and carbohydrates from defatted flaxseed meal. Key processing conditions included temperature (130, 160, 190 °C), solvent pH (4, 6.5 and 9), solvent to solid ratio (90, 150 and 210 mL/g) and introduction of co-packing material (0 and 3 g glass beads). The addition of 3 g glass beads increased the yields for all target compounds. The maximum yield of lignans (21 mg/g meal) was obtained at 170 °C with solvent to solid ratio of 100 mL/g meal at pH 9. Optimal conditions for protein extraction were pH 9, solvent to solid ratio of 210 mL/g meal and 160 °C. Total carbohydrates recovery was maximized at 215 mg/g meal (50% recovery) at pH 4 and 150 °C with solvent to solid ratio of 210 mL/g meal. The increase of temperature accelerated extraction, thus reducing solvent volume and time to reach equilibrium. For the extraction of proteins and carbohydrates, however, a temperature of 130-160 °C is recommended, as proteins and carbohydrates are vulnerable to thermal degradation. 相似文献
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本研究建立了一种磁力搅拌辅助的超临界CO2萃取法,用于提取五味子中的8种木脂素类化合物,采用高效液相色谱对目标分析物进行分离和测定。本研究以超临界CO2作为萃取溶剂,采用乙醇溶液预先浸渍样品,通过磁力搅拌促进样品与超临界CO2的充分接触,以提高传质效率。本研究对影响木脂素类化合物提取率的各个因素进行了优化,并与传统方法进行了比较。实验结果表明,各目标分析物在线性范围内具有良好的线性关系(r>0.9998),检出限和定量限为21.30~990.20 ng/mL和60.80~2992.80 ng/mL,加标样品回收率在75.00%~97.78%之间,分析结果令人满意。与其他方法相比,虽然本法在提取时间和实验成本上并不占优势,但本法所获得的提取率明显高于微波提取法和热回流提取法,并与药典方法相似,尽管本法在萃取过程中使用了少量的有机溶剂,但料液比仅为1:2,是其他方法的1/25~1/50。本法具有样品处理量大、操作简单、绿色环保、萃取效率高等优点,在工业化生产应用方面具有一定潜质,可用于中药中木脂素类化合物的提取分离。 相似文献
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The pressurized liquid extraction and HPLC-DAD was developed for extraction and determination of bioactive lignans in Schisandra chinensis. The efficient PLE conditions employed methanol as extraction solvent, 125 °C of extraction temperature, 5 min of static extraction time and only one cycle. A rapid HPLC-DAD method was described for simultaneous determination of nine lignans, including schisandrol A, gomisin J, schisandrol B, tigloylgomisin H, angeloylgomisin H, schisandrin A, γ-schisandrin, gomisin N and schisandrin C. The extraction efficiency of PLE was observed to be comparable with reflux and sonication. In addition, the contents of nine lignans in S. chinensis from different regions were analysed by PLE and HPLC-DAD method. 相似文献
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《Journal of food engineering》2007,78(4):1087-1095
The application of pressurized low polarity water (PLPW) extraction to the production of flaxseed lignans-rich products has been studied, and the key geometric and process conditions, including temperature, flow rate, and total volume have been determined and optimized. Maximum amounts of lignans and other flaxseed bioactive, including proteins were extracted at 160 °C and 5.2 MPa. However, on a dry weight basis the most concentrated extracts in terms of lignans and other phenolic compounds were obtained at 140 °C and 5.2 MPa. A flow rate of 0.5 mL/min was optimal for the extraction of lignans from flaxseed (Linum usitatissimum L.) and a total volume of 30–40 mL/g of seed was required to maximize the recovery. Higher flow rates increased the rate of the extraction but required larger water volumes. Bed depth to ID ratios of 5–18 resulted in faster extraction and maximum recovery (90–95%) at water to seed ratios of 30–50 mL/g. Larger depth to ID ratios (15–18) would allow the use of lower solvent to solid ratios (14–20 mL/g) and would still result in yields of 84–90%. 相似文献
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以五味子种子为原料,提取溶剂为乙醇,采用超声波辅助提取工艺,并设计正交实验,优化得到了最佳的超声波提取工艺条件,然后以提取的五味子木脂素浸膏为原料,研究了吐温80、聚甘油辛癸酸酯、亲水性辛癸酸甘油酯对木脂素浸膏的乳化稳定性。研究结果表明,五味子木脂素的最佳提取工艺为:乙醇浓度90%,料液比(m:v)为1:8,提取时间为每次50min,提取次数为2次。在此条件下五味子木脂素的提取率为3.06%;木脂素乳化回填的最佳工艺为:每100mL乳液添加29.2mg木脂素浸膏,三种乳化剂用量分别为:吐温80,16.7mg;聚甘油辛癸酸酯,41.7mg;亲水性辛癸酸甘油酯,41.7mg。木脂素乳化回填后的溶液有较好的澄清度,并且在常温下存放60d未出现破乳现象,有较好的稳定性。 相似文献
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对引进的De-Smet浸出车间混合油蒸发、溶剂冷凝回收、尾气回收及水化脱胶系统的工艺配备情况及操作过程进行了介绍。整个蒸发系统采用全负压工艺技术,充分考虑余热利用,降低蒸汽消耗。从使用3年的情况来看,脱胶油含磷量和残溶达标,预处理车间和浸出车间整条生产线蒸汽消耗为265 kg/t,溶剂消耗小于等于0.8 kg/t。 相似文献
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超临界CO2提取五味子木脂素的工艺研究 总被引:2,自引:1,他引:1
目的研究超临界CO2流体提取(SFE-CO2)北、南五味子木脂素的工艺。方法以五味子总木脂素提取率为考察指标,以提取压力、提取温度、CO2流量等3项因素设计3个水平进行正交试验设计,确定最佳工艺。用薄层扫描色谱法测定提取物中4种主要木脂素(五味子甲素、五味子乙素、五味子酯甲和五味子醇甲)的含量,并与传统的乙醇提取法比较。结果超临界CO2流体提取五味子木脂素的优选工艺为:提取压力25 MPa,提取温度35℃,CO2流量2 L/min。北、南五味子总木脂素的平均提取率分别为93.2%、95.5%,提取物纯度分别为13.0%、9.2%,北五味子提取物中五味子乙素含量2.89%。结论 SFE-CO2法提取效率高、选择性好、操作简便,明显优于传统的乙醇提取法,可用于提取五味子木脂素。 相似文献
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本研究以提油率为评价指标,在萃取时间、溶剂用量、温度、pH四个单因素试验的基础上,利用响应面分析法对主要影响因素萃取时间、温度、pH,以乳化率为评价指标,对酶法水解提取鲐鱼鱼油的萃取条件进行优化。结果表明,提油率最高而乳化率最低的最佳萃取条件为萃取时间15min、温度40℃、pH4.0、萃取剂用量100ml/每20g原料。 相似文献
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萃取法提取谷维素的研究 总被引:1,自引:0,他引:1
:采用非极性溶剂萃取法对提取谷维素进行了试验研究 ,结果表明该方法可行。正交试验得到的最佳工艺参数为 :萃取时pH =8 5 ,二道捕集碱炼的超量碱为 6 0 % ,溶剂为苯 ,头道碱炼的保留酸值为 5 相似文献