地塞米松对胃癌细胞核骨架-中间纤维系统的影响

本文应用选择性抽提整装电镜观察,研究地塞米松诱导人胃癌ＭＧｃ８０－３细胞分化过程中细胞核骨架－中间纤维系统的构型变化。ＭＧｃ８０－３细胞核骨架纤维和中间纤维数量较少、分布不均匀,核纤层为厚薄不一结构,与两类纤维联系不密切。经１０μｇ／ｍｌＤｅｘ处理后,细胞核骨架纤维和中间纤维数量增多、结构层次丰富、单丝成份多,分布均匀并相互交织成规则网络,两类纤维通过薄层均一的核纤层密切联系,形成贯穿整个细胞核质     

HMBA对人胃腺癌细胞HM—IF系统形态的影响

本文研究了ＨＭＢＡ诱导处理前后人胃腺癌ＭＧｃ８０－３细胞的核骨架－中间纤维（ＮＭ－ＩＦ）系统形态的变化规律。整装扫描与透射电镜观察显示，ＭＧｃ８０－３细胞的核骨架及中间纤维表现为排列紊乱、联系疏松和分布不均，两类纤维之间则以厚薄不一的核周层状体相连结；在诱导处理之后的细胞中，这两种骨架纤维大多变得细长、丰富、排列有序且联系紧密，两种纤维间又通过薄而均一的核周层状体结构密切相闻，在整体上形成一种贯穿     

人胃腺癌MGc80—3细胞的核骨架——中间纤维系统

人胃腺癌ＭＧｃ８０—３细胞的核骨架──中间纤维系统倪子绵，李祺福（厦门大学分析测试中心电镜室，细胞生物学研究室，厦门３６１００５）细胞核骨架的结构与功能变化在细胞的增殖分化活动中起着重要作用￣［１］。一些研究报道，肝癌等多种肿瘤细胞的核骨架构型和蛋白...     

HMBA诱导胃癌细胞表面及骨架结构的变化研究

本文研究5mM HMBA(环六亚甲基双乙酰胺)诱导处理人胃腺癌MGc80—3细胞系后,细胞骨架及表面形态结构的变化。扫描电镜观察显示,MGc80—3细胞多呈球型,细胞表面有丰富的微绒毛,边缘有大量的丝状和片状伪足(图1)。经HMBA诱导后,细胞表面变化显著,主要表现在细胞表面微绒毛和边缘伪足的大量减少甚至消失,出现一些突起或皱痕状结构。细胞的形态普遍趋于扁平铺展状,呈现出与人正常胃粘膜原代培养细胞相似的表面形态结构(图2)。细胞骨架整装电镜观察显示,MGc80-3细胞骨架结构松散,分布不均,纤维间常有断开和分叉现象(图3)。经诱导后细胞骨架纤维伸长,纤维结构致密、分布均匀,纤维间联接紧密,并有较多的纤维束状结构(图4),与对照组细胞有着明显差异。     

两种卟啉类光敏剂对SMMC-7721细胞敏化损伤的拉曼光谱分析

用拉曼光谱技术比较血卟啉单甲醚HMEM和血卟啉衍生物HpD对肝癌细胞SMMC-7721光敏化损伤。实验测定了用两种光敏剂对SMMC-7721细胞光动力作用后的DNA和蛋白质的光谱图。结果显示:HMME和HpD对DNA和蛋白质构象的光敏损伤特征是相同的,但损伤程度不同。经HMME作用的SMMC-7721细胞的谱线,DNA各峰的位置和强度的变化都比HpD大,有的峰消失,蛋白质主链的几条谱线强度与HpD谱线相比大幅降低,表示HMME对DNA和蛋白质主链的损伤较大。结果表明:HMME对SMMC-7721细胞的光敏     

巴西菇多糖对肝癌细胞株凋亡的影响及其机制

目的 :研究巴西菇多糖诱导人肝癌细胞株 (SMMC-772 1)凋亡的作用及机制。方法 :采用电镜、DNA电泳、免疫组化法 ,对SMMC-772 1株细胞凋亡以及凋亡抑制基因 bcl-2的表达进行检测。结果 :SMMC-772 1株细胞经巴西菇多糖处理后出现核固缩、膜起泡、凋亡小体形成 ,琼脂糖凝胶电泳呈现出典型凋亡 DNA梯状带 ,细胞内 bcl-2表达较对照组低。结论 :巴西菇多糖能诱导 SMMC-772 1株细胞发生典型凋亡 ,其抑制细胞内 bcl-2的表达 ,是诱发肿瘤细胞凋亡的机制之一。     

二种固定方法对扫描电镜用植物细胞冷冻割断样品的影响

本文用冷冻割断法在扫描电镜下观察了大蒜(Allium sativum L.)根尖分生组织细胞内部的三维结构。样品制备采用了两种固定方法:冷冻割断前只用1％的锇酸固定的材料易在细胞质和细胞核之间断开;而先用Bouin‘s液固定,再用1％锇酸固定的材料易使细胞核被断开。据此认为前一固定法适于研究细胞质的内部结构(如细胞骨架纤维、线粒体、内质网等)及其三维分布关系;后一种固定法适于核内结构(染色质、核仁、核基质纤维)的三维图象的研究,特别是核仁纤维中心染色质的三维结构。     

还精煎对老年小鼠肝细胞亚微结构的影响

本实验用纯种小白鼠分成三组:正常对照组、老年组、还精煎组。动物迅速杀死后,取肝组织用电镜常规技术作固定、脱水、包埋和切片,铅轴染色,DXA_4—10电镜观察。对照组动物肝细胞超微结构正常,肝细胞多为三角形,含1—2个核,核膜清楚、核红周围有核红相随染色质围绕,异染色质分布在核膜内层,常染色质均匀分布于核液内。细胞质内有丰富的细胞器及内含物,线粒体嵴清楚,粗面内质网平行排列成群,滑面内质网分布于细胞质中,高尔基体分布于核附近及胆小管处,此外尚有溶酶体、微粒、肝糖元、少量脂滴及色素。老年组动物肝细胞的核及线粒体的亚微结构和核糖体的含量有显著变化。细胞的核仁失去典型的核仁丝结构,出现核仁病     

鼻咽微小浸润癌的电镜观察

15例鼻咽活检组织病理诊断为慢性炎症,而电镜检查却发现二例有微小浸润癌(其中一例数月后病理理诊断为鼻咽癌)。此二例均是在非典型鳞状化生的基础上发生的,其底层具异型性的化生细胞通过缺损的基底膜向上皮的固有层浸润,浸润的方式多以伸出不同形状的伪足为特点,伪足还可有多级分支,末级分支处的胞膜往往模糊不清,这可能与其以阿米巴运动的方式使整个细胞侵入上皮的固有层有关。浸润的癌细胞3—5成群或索状排列形成微小的癌巢,巢内的癌细胞多具丝状、指状、翼状及球状突起,细胞间由胞浆突起互相疏松的连接,有时可见其间有发育不良的桥粒。癌细胞核大,常染色质丰富,核仁大而居中或靠核膜,胞浆基质电子密度致密或透亮,细胞器较少並多位于核周,有数量不等的胞浆微丝或张力原纤     

间变性大细胞微绒毛淋巴瘤的超微结构观察及鉴别诊断

目的：探讨间变性大细胞微绒毛淋巴瘤的超微结构特点及鉴别诊断。方法：结合光镜、免疫组化及临床资料,对2例间变性大细胞微绒毛淋巴瘤进行超微结构观察。结果：光镜下,肿瘤呈窦性、巢团状浸润生长,瘤细胞排列紧密,体积大,核仁清楚。免疫组化显示瘤细胞CD30、EMA阳性。电镜下,瘤细胞表面有大量长短不一,粗细不等的微绒毛。胞质内有少量线粒体、核糖体、粗面内质网及高尔基复合体,1例偶见溶酶体样颗粒。核不规则,核仁明显,无细胞连接、基底膜及黑色素小体。结论：间变性大细胞微绒毛淋巴瘤的超微结构特征易与转移性低分化腺癌、恶性间皮瘤、黑色素瘤及毛细胞白血病鉴别。     

Improved ultrastructure of the desmosome-intermediate filament complex in MCF-7 breast cancer cells

We report on the application of variations to the traditional TEM processing methods, which provide improved clarity of the desmosome-cytoskeleton complex of MCF-7 human breast cancer cells. A more comprehensive understanding of the ultrastructure is presented, which in the past has been demonstrated in diagrammatic form based on numerous electron micrographs. Ultrastructural analysis shows that intermediate filament bundles do not terminate at the desmosome structure, but instead are continuous into the cytoplasm. Furthermore only a minor proportion of individual filaments are in actual contact with the desmosome plaque. Intermediate filaments were also observed throughout the cytoplasm and to the surface of the nuclear membrane. Extraction protocols allowed clear identification of other cellular features such as nuclear pores, which are approximately 80-85 nm in diameter, and were best viewed in sections cut tangentially to the nuclear surface.     

两种小菜蛾寄生蜂毒液器官超微结构的比较

应用超薄切片和电镜技术，观察比较了两种小菜蛾的内寄生蜂——小菜蛾盘绒茧蜂和半闭弯尾姬蜂毒液器官的形态结构。两种蜂的毒液器官均由一个毒囊和两条毒腺组成，但毒腺与毒囊的连接位点不同，毒腺分别接于毒囊的基部和顶端。它们的毒腺均由分泌细胞、退化的外胚层细胞和环腔的内膜三层结构构成，分泌细胞内含司分泌毒液的囊状细胞器及大量的内质网、线粒体、高尔基体、溶酶体和液泡。半闭弯尾姬蜂的分泌细胞内有分支的细胞核。小菜蛾盘绒茧蜂的毒囊由肌肉鞘和扁平细胞层构成，内膜呈波浪状内突并沿囊腔有几丁质增厚；半闭弯尾姬蜂的毒囊由肌肉鞘、分泌细胞和扁平细胞三层构成，分泌细胞基部内陷成一系列大的突起，这些结构与雌蜂的分泌和排出毒液的行为密切相关。在衰老的小菜蛾盘绒茧蜂的腺细胞中还存在大量的髓样体。本文就两种寄生蜂毒液器官的生物学、细胞生理学及相关的分类进化的意义进行了讨论。     

Experimental investigation of non-linear processes in quasi-optical resonator with paramagnetic

The following papers [1,2] are devoted to the experimental investigations of electron system in polarized nuclear target (PNT) materials and in particular in such substance as a paramagnetic complex HMBA (Cr^v). The processes of electron and nuclear system interactions in these matters were also considered [3,4]. The purpose of a present paper is the study of a nuclear system paramagnetic complex HMBA (Cr^v) in a 1,2 - propylene glycol. The super high frequency (SHF) power of saturation is changed during our experiment.     

细胞核凋亡过程中核基质的变化

在非细胞体系中以细胞色素C作为诱导剂，诱导小鼠有细胞核发生凋亡，在透射电子显微镜及扫描电子显微镜下观察到凋亡的一现典型的凋亡形态学特征，整装电镜的结果显示，在凋亡过程中核基质结构被破坏，但核基质结构仍然存在，并最终形成凋亡小体的结构基础。     

Non-linear quasi-optical cell with polarized nuclear target (PNT) material

A resonance electromagnetic structure (a Fabry-Perot resonator) with a plane-parallel sample of the polarized nuclear target (PNT) material of a paramagnetic complex Na HMBA (Cr^V) is considered under condition of dynamic polarization of nuclear by millimeter waves. Non-linear effects observed at the electron spin resonance (ESR) in such structure have been studied. The results of the experiment with the quasi-optical resonance cell of the radiospectrometer designed for the investigation of the magnetic resonance in the millimeter wavelengths range are given. A hysteresis of the transfer coefficient of the cell in the ESR-line has been found while slow passing the ESR-line by retuning of the static magnetic field. The deformation of the form of non-stationary signals passed through the cell has been found.     

电子显微镜在疑难病例病理诊断中的应用

目的：通过对4例疑难病例的诊断,进一步探讨电镜在疑难病例诊断中的作用及意义。方法：采用电镜技术对4例疑难病例进行超微结构观察。结果：Merkel细胞癌含有许多神经内分泌颗粒,Wilson’s病肝细胞溶酶体吞噬较多铜颗粒;毛细胞白血病的细胞表面有许多长突起,甲状腺玻璃样小粱状腺瘤细胞胞质内有成束中间丝等。结论：电镜对疑难病例的确诊具有重要价值。     

甲状腺乳头状癌的超微结构观察

对３０例甲状腺乳头状癌进行了详尽的超微结构观察与分析,通过与６例甲状腺滤泡癌和２０例甲状腺腺瘤的对比观察与分析,提出了甲状腺乳头状癌细胞的超微结构特征,主要表现为核大,分叶核,丰富的常染色质,极少的核膜下异染色质及突出的核仁等。电镜观察为乳头状癌的超微病理诊断提供了有力的依据。     

激光等离子体丝阵列对10 GHz微波传输特性的影响

为了研究飞秒激光等离子体丝阵列对10 GHz微波传输特性的影响,利用COMSOL软件构建了飞秒激光等离子体丝阵列与微波相互作用的数值仿真模型,研究了等离子体丝阵列参数、等离子体特征参数、阵列层数对微波反射率和透射率的影响。数值结果表明:当微波的电场方向垂直于等离子体丝轴向时,无论微波相对于丝阵列的入射角如何变化,丝阵列对微波完全没有影响。增加丝的直径或者电子数密度、减少阵列间距或者电子温度都可以使反射率增加,透射率减小。光丝直径为500m,阵列间距为1 mm的等离子体丝阵列对10 GHz微波反射率最大可达到0.88,此时等离子体的特征参数为ne=11023 m-3,Te=0.3 eV。当增加丝阵列的层数时,透射率减小,最终趋近于0,而反射率则保持不变。该研究结果对飞秒激光等离子体丝阵列屏蔽干扰微波具有重要意义。     

Ultrastructural verification of anchoring role of lamina fibroreticularis of dental basement membrane in odontogenesis

In a previous study of the developing tooth a characteristic fibrillar layer associated with the basement membrane of the inner enamel epithelium was found to be a highly specialized lamina fibroreticularis of the basement membrane which is unusually rich in basotubules, 10 nm wide microfibril-like structures. In this study this layer was further examined in detail in the hope of ultrastructurally elucidating its role in odontogenesis. Tooth germs of the monkey (Macaca fuscata) were processed for thin section observations. Dental papilla cell processes were inserted into the lamina fibroreticularis and their surface was closely associated with numerous parallel basotubules. With high-resolution observations the space between the surface and nearest basotubules as well as the spaces between neighbouring basotubules were bridged by 1.5-3 nm wide filaments running perpendicular to the axis of basotubules. These results indicate that the processes of dental papilla cells are linked to groups of basotubules by means of 1.5-3 nm wide filaments. Immunoperoxidase staining showed the presence of fibronectin along basotubules as well as within the space between the process and basotubule. This result, together with the comparison of these filaments with microfibril-associated 1.2-3 nm wide fibronectin filaments and the reported abundance of fibronectin in the basement membrane area during odontogenesis, indicates that these 1.5-3 nm wide filaments are composed of fibronectin. After immunostaining for amyloid P component, done with the rat tissue because of the nature of an available antiserum, basotubules in the lamina fibroreticularis were positively stained, as has been shown in basotubules/microfibrils in other locations. Microfibrils function as anchoring rods by interlinking connective tissue components to one another and to the cells. Basotubules, thought to be basement membrane-incorporated microfibrils, in the lamina fibroreticularis in this study are also likely to function as an anchoring device to immobilize dental papilla cells along the basement membrane. Such an arrangement of mesenchymal cells is known to be crucial for the successful differentiation of odontoblasts in the developing tooth.     

Fine structure of mitochondrial helical filaments revealed by computer image analyses

In order to clarify the fine structure of the helical filaments appearing in the outer compartment of the mitochondria, Sprague-Dawley strain male rats were given 30% ethanol in drinking water for 90 days. The hepatic tissues of these animals were fixed with perfusion of glutaraldehyde via the portal vein followed by immersion in OsO4 and were then routinely processed for preparation of thin sections. Transmission electron micrographs of the sections were used for computer image analyses of the intramitochondrial helical filaments. With the application of the image analysis, it was revealed that the helical filaments show a right-hand rotating helix of 4.3 nm in thickness, 13.2 nm in diameter of the helix, and 15.7 nm in pitch. Also, with the multivariate statistical analysis and classification method of the IMAGIC image processing system, it was suggested in the classified images that the helical filament has substructures of rod-shaped particles of 4.3 nm in diameter. These particles are considered to be connected to each other and forming the helical structure of the filament.