苦荞种子中葡萄糖含量变异研究

[目的]明确不同产地苦荞种子中萄萄糖含量的差异.[方法]以不同产地的35份苦荞资源为试材,测定了其籽粒的葡萄糖含量.[结果]35份苦荞资源的葡萄糖含量变化幅度为0.0556%～0.8402%,平均值为0.3217%;不同产地苦荞的葡萄糖含量存在差异,以贵州威宁的苦荞种子T324中葡萄糖含量最高,原产贵州威宁苦荞种子T398最低.[结论]为明确葡萄糖含量在不同苦荞资源间的遗传变异规律提供了参考.     

苯酚-浓硫酸法测定西藏天麻中多糖含量的条件优化

[目的]建立西藏天麻(RHIZOMA GASTRODIAE)多糖含量的测定方法.[方法]采用苯酚-浓硫酸比色法进行测定.[结果]以葡萄糖为标准品,苯酚-硫酸法测定天麻多糖含量的最佳反应条件为:6%苯酚溶液1 ml,浓硫酸5 ml,水浴温度25℃.西藏天麻多糖以葡萄糖(C<,5>H<,12>O<,6>)计,在21%～33%之间,平均值为28%,多糖的含量高,具有较高的使用价值和经济价值.[结论]该研究中的测定方法简便、准确,可用于评价西藏波密天麻药材的质量.西藏天麻多糖的含量较高,是一种大有潜质的保健食品和药材,开发及利用西藏天麻,对带动当地经济有非常重要的意义.     

反相高效液相色谱法同时测定香椿果中3种酚性化合物

[目的]建立了同时测定香椿果提取物中没食子酸、没食子酸甲酯、1,2,3,4,6-五-O-没食子酰-β-D-葡萄糖3种酚性化合物含量的方法.[方法]采用Hypersil C<,18>色谱柱(6.0 mm×150.0 mm,5 μm),流动相为甲醇-0.1%甲酸,以1.0 ml/min流速为进行梯度洗脱,柱温为30℃,检测波长为280 nm,外标法定量.[结果]没食子酸、没食子酸甲酯、1,2,3,4,6-五-O.没食子酰-β-D-葡萄糖分别在0.048～0.480、0.018～0.180和0.022～0.220 μg之间线性关系良好(r≥0.999 8),平均加样回收率分别为101.6%、99.5%和99.1%,RSD值≤2.9%.[结论]该含量测定方法简便、准确、分离效果好,可用于香椿果中的酚性成分含量的测定.     

HPLC测定不同采收期川楝子中阿魏酸的含量

[目的]测定不同采收期川楝子中阿魏酸的含量,并考察阿魏酸在采收期内的含量变化.[方法]采用高效液相色谱法,色谱柱为UltrasphereTM-ODS柱(4.6 mm ×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液(15:85=V:V),流速为1.0 ml/min,检测波长为322nm,柱温为25℃.[结果]不同采收期川楝子药材中阿魏酸含量无显著变化,以1月份采集的样品中阿魏酸含量最高.[结论]该方法简便、快速,可为川楝子确定最佳采收期提供科学依据.     

分光光度法测定伏牛山荷叶中微量元素锌

[目的]测定荷叶(FOLIUM NELUMBINIS)中微量元素锌的含量,为荷叶在医药及食品工业的深度开发利用提供科学依据.[方法]采用直接分光光度法,以1-(2-吡啶偶氮)-2-萘酚(PAN)为显色剂、锌标准液为对照品,在562 nm波长处测定吸光度.[结果]Zn(Ⅱ)与PAN形成红色的络合物,在0.48～5.03 μg/ml范围内,吸光度值与含量线性关系良好(r=0.999 9);平均回收率为99.8%,RSD为0.34%.伏牛山荷叶中锌元素的平均含量为87.44 μg/g,RSD为1.02%.[结论]该方法简便、准确、重现性好,稳定性高,可用于荷叶中微量元素锌含量的测定.     

RP-HPLC法测定土藿香根中乌索酸和齐墩果酸的含量

[目的] 建立反相高效液相色谱法同时测定土藿香(Agastache rugosa)根中乌索酸和齐墩果酸含量的方法,为土藿香的研究开发提供试验依据.[方法]色谱柱为C18分析柱(4.6 mm×250.0 mm,5 μm),流动相为乙腈- 甲醇-0.3% H3PO4水溶液 (V∶V∶V=60∶30∶10),流速为0.8 ml/min,检测波长为210 nm,柱温为常温.[结果]在选定色谱条件下2种成分线性关系良好,乌索酸和齐墩果酸的样品加样回收率分别为99.6%和98.9%,RSD分别为3.12%和1.99%.土藿香根中乌索酸和齐墩果酸的平均含量分别为0.138 mg/g和0.147 mg/g.[结论]该方法快捷、准确、可靠,可用于土藿香根中乌索酸和齐墩果酸含量的定量分析,为土藿香药用价值的开发提供了试验依据.     

紫外分光光度法测定垃圾渗滤液中的DEHP含量

[目的]建立紫外分光光度法测定垃圾渗滤液中DEHP含量的方法.[方法]以三氯甲烷为溶剂萃取50 ml垃圾渗滤液样品中的DEHP,然后利用紫外分光光度法进行测定.[结果]DEHP浓度的线性范围为30～100 mg/L,其标准曲线方程Y=0.003x+0.015 1,相关系数R<'2>=0.998 8,加标回收率为92.02%～102.93%.对标准DEHP溶液进行6次平行测定,RSD为0.045%～0.164%.[结论]该测定方法操作简便,有较高的准确度,能够满足垃圾渗滤液中DEHP含量的测定,具有一定的应用价值.     

紫外可见分光光度法测定不同年份黄精中多糖含量

[目的]测定九华山地区所产不同育成年份黄精中多糖含量,为九华山地区黄精的产品分级和种植研究提供参考.[方法]采用苯酚-硫酸法对九华山地区产不同年份黄精中多糖含量进行测定.[结果]不同年份的黄精中多糖含量变化较大,随着种植年限的增加,多糖含量呈现升高趋势.其中,1年生黄精中多糖含量最少,为13.02%;9年生多糖含量达到最大,为18.44%;10年生多糖舍量出现下降,为15.64%.[结论]可以在实际采收中,通过不同育成年份对黄精进行分级.     

RP-HPLC法测定当归片中阿魏酸的含量

[目的]建立当归片中阿魏酸含量的RP-HPLC测定方法.[方法]采用反相高效液相法,以阿魏酸对照品作为内部标准品测定自制当归片中阿魏酸的含量.[结果]阿魏酸在8.3～41.6μg/ml范围内呈现良好线性关系,加样回收率为99.9%,RSD=0.51%(n=6).[结论]高效液相法操作简单、准确、重复性好、精密度高,可作为当归片的质量控制方法.     

微波灰化-原子荧光光谱法测定卷烟纸中As含量

[目的]建立一种用于测定卷烟纸中砷含量的微波灰化-原子荧光方法.[方法]采用微波灰化法处理卷烟纸样品,然后用原子荧光光谱法测定卷烟纸中砷含量.[结果]砷在O.2～20 μg/L范围内和荧光强度有良好的线性关系,其回归方程为If=286 C(μg/L)+2.68(r=O.999 5).该方法的回收率为95.4%～97.0%,检出限为0.04 μg/L.[结论]采用原子荧光光谱法测定卷烟纸中的砷含量,灵敏度高,检出限低,精密度好,回收率高,可满足卷烟纸中痕量砷测定的要求.     

转炉炉壳热应力分析

根据实际转炉建立实体模型。以有限元为手段,考虑了炉衬和炉壳材料的物性参数随温度变化的特点和炉衬与炉壳之间膨胀间隙,计算了转炉炉壳在温度载荷和炉衬膨胀压力同时作用下的热应力。所得结果可为转炉炉壳设计提供依据。     

Quality of life of relatives of mental patients

Usage of quality of life of psychiatric patients' relatives as the parameter of estimation of the quality of psychiatric care is considered. By means of standard questionnaire elaborated by the authors as well as by means of both individual and group conversations the quality of life was analyzed in 40 relatives which lived together with psychiatric patients. Different spheres of their life and difficulties (financial, social-psychologic, production) were examined in families with such patients. The defects of psychiatric care that had direct influence on the quality of life of the psychiatric patients' relatives are outlined.     

Effect of antibiotics of chemotaxis of human leukocytes

The effect of 20 different antibiotics on chemotaxis by human neutrophils was studied. Human leukocytes incubated with chloramphenicol, rifampin, sodium fusidate, and tetracyclines in vitro showed markedly depressed migration. The mechanisms by which these antibiotics affect leukotaxis are discussed.     

显微维氏硬度测量不确定度评定

对显微维氏硬度测量不确定度进行评定，建立了不确定度计算的数学模型，确定了影响实验结果的各项因素，计算出了各因素的标准不确定度，得出结果的扩展不确定度，并给出最终测量结果的表达式。     

Concept of freedom of infection of animal flocks

Totally negative results of epidemiological investigation of random samples do not prove the absence of the infection as the pathogen may be restricted to only a few animals in the herd for a long time. The statement "absence of infection" is critical for such situations. The question is raised, whether the statement "absence of infection" should be generally avoided. Classification of herds and flocks according to the prevalence of the pathogen would be more valid for the implementation of control measures.     

The effect of amount of possession of the trait of hostility on accuracy of perception of hostility in others.

Some theories of personality hold that an individual's perception of others is influenced by his own personality characteristics; more specifically, that a trait of low social value will be projected more into others, and that insight into the possession of these characteristics reduces this distortion. These 2 hypotheses were tested by having a group of college students rate each other (including themselves) on degree of friendliness or hostility. Agreement with group rating about self yielded the insight score. In general, the hypotheses were supported; the "friendly" Ss were most accurate in their perception of others, and insight was positively associated with accurate perception. From Psyc Abstracts 36:04:4HL16M. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Comparison of rates of infection of two methods of emergency ventricular drainage

The rates of infection of two methods of external ventricular drainage in use at Atkinson Morley's Hospital--namely, (a) percutaneous drainage with Rickham reservoirs and (b) tunnelled ventriculostomies--were compared in this retrospective review. Percutaneous drainage of CSF with Rickham reservoirs was associated with a 27% rate of infection as identified by positive microbiological cultures; tunnelled ventriculostomy catheters had a 10% infection rate. The difference in the infection rate between the two methods was statistically significant (P < 0.015). Other variables examined, including the age and sex of the patients and the reasons for ventricular drainage, were not associated with an increased rate of infection. Most infections from either method were caused by a coagulase negative staphylococcus. The average duration of ventricular drainage before identification of positive cultures was 5.7 days for Rickham reservoirs and 6.0 days for ventriculostomies.