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简单介绍了胶原蛋白的结构、来源和其在化妆品中的功效,重点阐述了胶原蛋白的酶解和分子量检测方法,对未来胶原蛋白在化妆品中的研究方向做出了展望。对于各种化妆品的不同需求,可根据不同分子量的胶原水解产物的不同功能特性予以满足,胶原蛋白因其特有的护肤美容功效而受到越来越多的关注。 相似文献
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采用高温酸解法对水剂、乳霜类化妆品进行前处理,经柱前衍生化法-高效液相色谱法测定样品中L-羟脯氨酸(L-Hyp)和胶原蛋白含量。化妆品样品经105℃下盐酸酸解后,以α-氨基丁酸为内标物,利用高效液相色谱法定量测定L-Hyp含量(色谱柱:Waters Xbridge C18,流速:2.0m L/min,检测波长:260 nm,柱温55℃),经换算得到样品中胶原蛋白的实际含量。色谱条件下,L-Hyp与内标物峰面积比和质量浓度比成良好的线性关系,相关系数为0.999 8。含量测定的相对标准偏差为0.06%~2.78%,加标回收率为93%~101%,精密度和稳定性良好。该方法准确可靠,适用于测定化妆品中的L-Hyp和胶原蛋白含量。 相似文献
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《日用化学工业》2015,(9)
通过水提取法分离提取得到牛肝菌提取液,生药质量浓度为60 g/L。采用XTT比色法检测牛肝菌提取液对表皮角质细胞的线粒体活性、观察细胞增殖并检测其对细胞DNA含量的影响;利用DCFH-DA等方法检测牛肝菌提取液对人皮肤成纤维细胞内活性氧(ROS)及抗氧化元件(ARE)的影响;利用免疫荧光法检测牛肝菌提取液对成纤维细胞Ⅰ型前胶原蛋白表达的影响。结果表明:牛肝菌提取液在终质量浓度为1.5 g/L时,较对照组可以显著提升角质细胞的线粒体活性及DNA的含量,能够明显促进细胞的增殖;牛肝菌提取液可以明显减少细胞内ROS的含量、提高细胞内ARE激活水平;同时牛肝菌提取液还可以促进Ⅰ型前胶原蛋白的表达。 相似文献
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胶原蛋白被广泛地作为生物医用材料使用,具有良好的生物相容性、组织再生功能和机械性能。为了探讨交联对胶原蛋白提取的影响,在体外酶降解胶原蛋白的实验中,通过胶原蛋白物理外观形态发生变化、降解液羟脯氨酸含量分析和红外谱图分析等表征方法,考察了经过戊二醛交联所获得不同交联度的胶原蛋白体外降解情况。同时,对其降解原理进行了进一步研究。实验表明经过0.2%戊二醛交联1h的胶原蛋白抗酶解性能最好,基本能满足生物医学材料的要求。 相似文献
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研究了冰川水和五脉绿绒蒿提取物对人真皮成纤维细胞(HDF-a)的作用。采用MTT法检测了冰川水和五脉绿绒蒿提取物对HDF-a增殖活性的影响,利用荧光试剂盒检测了二者对HDF-a内ATP水平的影响,利用酶联免疫吸附试验(ELISA)法检测了二者对HDF-a细胞外基质中透明质酸含量的影响,利用Western Blot法研究了二者对HDF-a细胞外基质中Ⅰ型胶原蛋白和弹性蛋白表达的影响。实验结果表明,与对照组相比,冰川水处理后HDF-a增殖速度加快、代谢水平提高、透明质酸含量上升、弹性蛋白表达上调,且差异有统计学意义。五脉绿绒蒿提取物对HDF-a的作用在上述方面更为明显,同时可以促进Ⅰ型胶原蛋白的表达。二者共同作用时效果更为显著。 相似文献
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《日用化学工业》2021,(8)
使用酿酒酵母对黑枸杞进行发酵,获得黑枸杞发酵液。从成分、安全性、抗衰老效果三个方面对其进行检测和评价,为其在化妆品领域的应用提供基础。首先从生化水平对黑枸杞发酵液清除自由基能力进行检测,接着从细胞水平评估黑枸杞发酵液对人成纤维细胞内的胶原蛋白、胶原蛋白酶以及抗氧化酶活性的影响进行检测,最后使用鸡胚尿囊膜和人体斑贴试验对黑枸杞发酵液的眼刺激性和皮肤安全性进行评价。结果表明,黑枸杞发酵液富含多糖和蛋白质,多酚、黄酮和原花青素含量较少。黑枸杞发酵液具有良好的清除DPPH自由基和ABTS+自由基的能力,IC50分别为8.15%和1.08%。此外,5%体积分数的黑枸杞发酵液能够促进胶原蛋白合成,含量为对照组的137.84%(p0.01),同时能够抑制胶原蛋白酶的表达,含量为对照组的70.41%(p0.01)。1%体积分数的黑枸杞发酵液能够极显著提升成纤维细胞内抗氧化酶活性(p0.01),提高总抗氧化能力以及抑制活性氧的生成(p0.01)。鸡胚尿囊膜试验和人体斑贴试验结果显示黑枸杞发酵液具有较高的眼睛安全性和皮肤安全性。 相似文献
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R. Marquard 《European Journal of Lipid Science and Technology》1987,89(3):95-99
Analytical Methods for Quality Traits in Plant Breeding In plant breeding programs selection for quality traits usually requires two kinds of methods, accurate quantitative methods for precise determination and rapid screening methods for mass selection. The demands on all methods are acceptable sensitivity and precision besides low costs and short times per analysis. In screening programs for fat and protein content NMR and NIR analysis are methods of choice. By own experiences, pyrolysis gaschromatography is a very useful tool for the determination of protein content. Rapid analysis of crude fibre content is still an unsolved problem, although there are many improvements of corresponding laboratory equipment. Paperchromatography is a common rapid method for fatty acid analysis. For quantitative determination gaschromatographic methods are very useful, especially in combination with automatic sample injection techniques. Some unsolved difficulties still exists with the analysis of glucosinolates. Screening-methods are based either on the colour formation of intact GSL molecules with palladium reagent or on the determination of enzymatically released glucose following myrosinase degradation of intact glucosinolates. Hitherto, quantitative determination of glucosinolates is based on gaschromatography as well. Unfortunately, these methods lack in determination of indole glucosinolates, due to their thermal instability. HPLC determination is well suited for the detection of both compounds, simultaneously. This is of great importance, because most of the breeding material low in total glucosinolate content shows remarkably increased indole glucosinolate proportions. For practical purposes, photometrical methods as well as X-ray fluorescence-spectrometry seems to be useful and rapid tools for the determination of total glucosinolate content. 相似文献
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The paper discusses the methods suitable for serial investigations of vegetable oils modification by catalytic isomerization, which comprises geometrical and positional isomerization, as well as partial polymerization and cyclization of unsaturated acids. The first part discusses the methods relating to the determination of stereoisomers. Infrared spectrophotometric method is used for the determination of isolated and conjugated trans isomers total content. Gas chromatography method in packed columns filled with cyanosilicones is suitable for the determination of the trans bonds in monoenes, dienes and trienes. In further parts of the paper discussion on the selection of methods of determination of the total content of conjugated dienes, cyclic monomers and polymers is envisaged. 相似文献
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氧化锌在国民经济建设中是不可缺少的重要基础化工原料和新型材料,随着近年来伪劣氧化锌的出
现,使得氧化锌现行标准的局限性日益显现。通过理论基础和实验验证,对氧化锌现行标准中锌含量测定方法的局限性进行了论证。中国现行氧化锌标准中锌含量的测定方法仅局限于纯品或近乎纯品的氧化锌。如果被测氧化锌是含有大量杂质金属元素的伪劣氧化锌,那么按国家标准或行业标准定量分析时,由于大量杂质金属元素的存在,超出了掩蔽剂掩蔽的限量,使得锌含量的检测结果高于实际锌含量。因此,中国氧化锌现行标准目前无法正确评价伪劣氧化锌的质量水平。目前,急需针对伪劣氧化锌的关键检测技术开展研究,制定可行有效的伪劣氧化锌的鉴别与氧化锌含量的检测技术。 相似文献
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Stephen A. Wise Lane C. Sander Michele M. Schantz 《Polycyclic Aromatic Compounds》2015,35(2-4):187-247
The identification of 16 polycyclic aromatic hydrocarbons (PAHs) as priority pollutants by the U.S. Environmental Protection Agency (EPA) in 1976 has been a primary driver for analytical methods development for the determination of PAHs. In this article, the historical development of methods in liquid chromatography (LC) and gas chromatography (GC) to separate these 16 PAHs is discussed. In LC a significant effort was the search for and the fundamental understanding of the unique stationary phase capable of achieving the desired separation of the 16 EPA PAHs. For GC methods, the focus on stationary phase development has been the separation of critical isomers with a broader scope than the 16 EPA PAHs. The current routine LC and GC methods for the 16 EPA PAHs are well established; however, new advances in analytical techniques beyond LC and GC are discussed. Many analysts are now interested in more than just the 16 EPA PAHs (e.g., higher molecular mass PAHs and alkyl-substituted PAHs) and analytical methods have emerged to address these needs. Reference materials and their use in the determination of PAHs are discussed. 相似文献
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目前催化裂化油浆固含量的测定方法没有统一的标准,现行测定方法的比较分析鲜有报道。为了给油浆固含量测定方法的选择提供参考,本文通过实验,系统地比较分析了过滤法、离心法、灰分法以及炭化灼烧法这4种方法在固含量测定方面的准确度和精密度,并对过滤法在过滤材料及稀释剂选择方面进行了进一步的优化分析。结果表明:过滤法的准确度和精密度均较高,且基本能全面反映油浆固含物中催化剂及焦粉的含量;离心法的准确度和精密度均较差,测定结果明显偏大;灰分法及炭化灼烧法的精密度较高,但准确度较低,测定结果偏小;采用过滤法测定固含量时,过滤材料选择慢速定量滤纸即可满足一般的精度要求,当精度要求较高时,过滤材料可选择孔径为0.22μm的有机滤膜,另外稀释剂可选择芳香性溶剂如甲苯。 相似文献
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Conclusions The informativeness of the methods of determining molecular weight distribution, determining the content of low-molecular fractions having a DP up to 100, and of determining the turbidity spectrum in the determination of microgel particle parameters in the identification of industrial lots of cellulose acetate has been demonstrated.The dependence of the spinnability of cellulose acetate solutions on molecular weight distribution and microgel particle parameters has been established.Translated from Khimicheskie Volokna, No. 1, pp. 37–38, January–February, 1987. 相似文献