共查询到20条相似文献,搜索用时 108 毫秒
1.
为了快速有效地检测植烟土壤中的烟草黑胫病菌的定殖数量,以一种锁核苷酸(LNA)引物为基础,进行了植烟土壤中烟草黑胫病菌数量的定量PCR 检测方法研究。结果表明:①与普通DNA引物相比,LNA 引物提高了PCR 反应的退火温度,减少了引物二聚体和非特异性产物的形成,提高了烟草黑胫病菌分子检测的灵敏度与特异性;②定量分析检测出14 个烟草-大蒜轮作土样中烟草黑胫病菌的定殖数量为2.76×103~5.20×104个/g,且在4~5 h 内完成检测,具有较高的灵敏度和检测效率。 相似文献
2.
3.
4.
为明确河南省烟草黑胫病菌群体的遗传结构,本研究利用SSR分子标记对来自河南省12个地区的32株烟草黑胫病菌进行分析。结果显示,6对SSR引物共扩增出24个条带,其中多态性条带23个,多态性条带比率为95.83%;Nei’s基因多样性指数和Shannon信息指数分别为0.2132和0.3356;遗传相似系数在0.61~1.00之间,相似系数0.80水平下UPGMA聚类可将32个菌株划分为5个类群,其中类群I和类群II包括29个菌株,为优势类群;主成分分析结果与UPGMA聚类分析结果基本一致;遗传结构分析推测河南省烟草黑胫病菌群体来自于3个祖先亚群,亚群I、亚群II和亚群III在群体中所占比例分别为13.59%、46.61和39.80%;单个菌株遗传构成分析显示87.50%菌株的遗传组分几乎由单一亚群组成。以上研究结果表明河南省烟草黑胫病菌群体的遗传多样性水平较低,遗传结构也较为简单。 相似文献
5.
转马铃薯Y病毒复制酶基因烟草的获得及抗病性分析 总被引:4,自引:0,他引:4
重组克隆DNA用KpnI/BamHI双酶切得到马铃薯Y病毒NIb基因,将其插入质粒pROK2相应切点中构成植物表达载体。用重组质粒pROK2转化农杆菌(Agrobacteriumtumefa-ciens)LBA4404菌株,叶盘法将NIb基因转入烤烟品种NC89的染色体,获得抗卡那霉素的转化再生植株。经抗性筛选、PCR检测、无性扩繁和大量重复抗病鉴定,结果表明,转化烟草植株DNA中整合了外源目的基因,且表现抵抗20μg/mlPVYN的侵染,ELISA分析认为抗性植株无病毒累积,初步筛选出对PVYN侵染具有较高抗性的转基因烟草植株。 相似文献
6.
7.
8.
9.
本试验筛选出了45个引物,可产生遗传多样性,其中OPA-08、OPA-16、OPC-10、OPC-12、GENMES'S 36号、78号、112号、181号引物其扩增结果稳定性好、多态性强。这些引物的RAPD扩增结果都可作为烟草烤烟品种的遗传多样性分析、基因定位及分子标记等。经凝胶电泳比较,GENMED'S 36号引物可作为烟草品种的最适宜的引物;1.8kb条带被初步认为与抗黑胫病基因相关。这些结果为以后对烟草烤烟品种在分子水平上的研究奠定了基础,加速烟草烤烟品种分子水平上的研究进程 相似文献
10.
用非程序DNA合成(UDS)试验以研究镰刀菌毒素的脱氧雪腐镰刀菌烯醇(DON)、雪腐镰刀菌烯醇(NIV)和黄曲霉毒素(AFB1)的联合毒性。结果表明,这三种毒素均可造成DNA损伤;DON和NIV及NIV与AFB1对DNA损伤有交互作用,产生增毒影响。 相似文献
11.
12.
双孢蘑菇SRAP、ISSR、RAPD标记遗传多样性和菌群分类研究 总被引:2,自引:1,他引:1
通过PCR试验,筛选能扩增清晰稳定的DNA条带的53对SRAP引物、10条ISSR引物和56条RAPD引物。SRAP、ISSR和RAPD 3种DNA分子标记扩增位点的平均多态率分别为70.21%、78.33%、61.94%。SRAP、ISSR和RAPD扩增结果用0与1表示,共获得1 046个位点的42 840个数据。采用组间距离法和Jaccard相似性系数,应用SPSS 11.0 for Windows软件进行聚类分析,构建40个双孢蘑菇样品的遗传关系树状图。当组间距离值取16时,40个双孢蘑菇品种可分为6个类群;当组间距离值取13时,40个双孢蘑菇品种被分为9个类群。 相似文献
13.
Ninety-nine randomly selected isolates of Listeria monocytogenes from several processing environment locations, in a shrimp processing plant, obtained during a 5-month sampling period were subjected to randomly amplified polymorphic DNA (RAPD) analysis with the use of four primers. Preliminary studies indicated that the number of DNA bands and their intensity differed greatly with respect to the commercial source of the Taq polymerase used with individual isolates. Eighteen composite RAPD types were discerned with the use of the four primers. Among these 18 composite RAPD types, type 1 comprised 14 indistinguishable isolates, and type 9 comprised 49 indistinguishable isolates. These results indicate that the shrimp processing plant was dominated by these 2 RAPD types that comprised 63.6% of the 99 randomly selected isolates. 相似文献
14.
Ninety-nine randomly selected isolates of Listeria monocytogenes from several processing environment locations, in a shrimp processing plant, obtained during a 5-month sampling period were subjected to randomly amplified polymorphic DNA (RAPD) analysis with the use of four primers. Preliminary studies indicated that the number of DNA bands and their intensity differed greatly with respect to the commercial source of the Taq polymerase used with individual isolates. Eighteen composite RAPD types were discerned with the use of the four primers. Among these 18 composite RAPD types, type 1 comprised 14 indistinguishable isolates, and type 9 comprised 49 indistinguishable isolates. These results indicate that the shrimp processing plant was dominated by these 2 RAPD types that comprised 63.6% of the 99 randomly selected isolates. 相似文献
15.
16.
Shioda H Satoh K Nagai F Okubo T Seto T Hamano T Kamimura H Kano I 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2003,44(4):203-207
Juice and integument of leaves of 3 Aloe species, Aloe vera, A. ferox and A. africana, are not allowed to be used as food according to the Pharmaceutical Affairs Law in Japan. On the other hand, whole leaves of A. arborescens can be used as food. The present study was designed to distinguish Aloe species by random amplified polymorphic DNA (RAPD) analysis. DNA was isolated from fresh and dried leaves of the 4 Aloe species. Five out of 32 different 10-mer primers examined were useful for analysis. By comparison of the characteristic bands of PCR products on agarose gel, it was possible to distinguish the 4 species. Thus, the botanical species of Aloe in commercial food products can be identified by RAPD analysis. 相似文献
17.
Junhua Zhang Yan Lin Yuchao Gu Jianjun Dong Wengong Yu 《Journal of the Institute of Brewing》2005,111(2):229-233
Random amplified polymorphic DNA (RAPD) was used for hop varietal identification, primarily to distinguish Tsingtaodahua, a fine Chinese variety. Eleven typical varieties, including four aroma hops, five bitter hops, Tsingtaodahua and Cluster, were successfully identified on the basis of 28 polymorphic RAPD bands amplified by five random primers. UPGMA analysis of RAPD data showed genetic relationship among analyzed varieties consistent with traditional hop classification. Subsequently, one specific RAPD fragment was converted to a sequence tagged site (STS) marker which can detect as little as a 5% admixture of the variety Kirin 1 in Tsingtaodahua. The RAPD and STS markers can be successfully used for Tsingtaodahua identification and quality control. 相似文献
18.
19.
不同来源酿酒酵母菌株的随机扩增多态DNA分析 总被引:2,自引:0,他引:2
研究中试用了20个随机引物对16株不同来源的酿酒酵母菌株全基因组进行了随机扩增多态DNA分析,其中OPG06,OPG11和OPG20三条适宜引物具有鉴别作用,每一引物均可扩增1~10条DNA片段,大多数片段分子量大小在100~2000bp之间,共扩增出34条RAPD谱带,多态性为85.3%,获得了稳定清晰的菌株RAPD指纹图谱。RAPD分析结果表明,不同来源的酿酒酵母菌株之间的遗传相似系数在37.5%~94.1%之间,反映出较高的遗传差异性,并可通过聚类分析将16株不同来源的酿酒酵母菌株按亲缘关系的远近分为6个类群。结果表明,利用RAPD标记技术在基因水平上对酿酒酵母菌株进行分子鉴定和分型是可行的。 相似文献