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细菌几丁质酶基因转化烟草的研究 总被引:1,自引:0,他引:1
为了提高烤烟品种K 32 6对真菌病害的抗性 ,以烟草再生植株的叶片为受体 ,采用农杆菌介导法将细菌几丁质酶基因导入烟草 ,获得了抗卡那霉素的转化植株 ,经PCR检测 ,初步证明了细菌几丁质酶基因已整合到烟草的基因组中 ;同时研究了再生烟草叶片的耐卡那霉素水平、受体预培养对转化的影响以及促进转化植株的生根等。结果表明 :培养基中卡那霉素浓度为 5 0mg/L时 ,能完全抑制烟草叶片的再生 ;烟草叶片预培养 2d的转化率提高 ;添加 0 .2mg/LIAA(吲哚乙酸 )能显著地提高转化植株芽梢的生根率。 相似文献
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《食品科技》2014,(7)
考察了脂肪酶生产菌株少根根霉N1023 G418的敏感性,结果表明,G418对少根根霉最小抑制浓度为500μg/mL。研究了不同条件对载体pBI121转化少根根霉的影响,结果表明,以根癌农杆菌LBA4404为介导菌,少根根霉N1023为受体菌株,G418为筛选标记的最佳转化条件为:乙酰丁香酮(AS)300μmol/L、根癌农杆菌的初始浓度OD600为0.6、共培养时间2 d。在此条件下pBI121对少根根霉的转化率达到112 cfu/106 cfu孢子。转化菌株经过5次连续培养后仍然稳定。通过上述研究,建立了根癌农杆菌介导载体pBI121转化少根根霉的方法。这是首次成功建立少根根霉的转化方法,为进一步的研究奠定了基础。 相似文献
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采用电击转化方法将双元载体质粒pCAM3300导入根癌农杆菌LBA4404,研究了不同实验条件对转化率的影响.结果表明:当电场强度为11 kV/cm时,转化率最高,每微克DNA得到9.8×103CFU;在细胞OD600为1.0时进行电转化,转化率最高,每微克DNA得到9.54×103CFU;在一定的细胞浓度范围内,电击转化率与细菌浓度密切相关,转化率随着细菌浓度的上升而上升;质粒大小与电击转化率之间没有明确的关系.将含pCAMBIA3300的根癌农杆菌与粗糙脉胞菌共培养转化,在含有膦化麦黄桐(PPT)(405 mg/L)的平板上筛选到转化子.PCR扩增转化子的染色体,初步证明Bar基因整合进粗糙脉胞菌的染色体中. 相似文献
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两种抗生素对甜菜离体叶柄分化的影响 总被引:4,自引:0,他引:4
在培养基中添加不同浓度的卡那霉素和壮观霉素,观察抗生素对甜菜叶柄分化再生的影响。结果表明:一定浓度的卡那霉素和壮观霉素对甜菜叶柄的分化均具有抑制作用,当卡那霉素浓度为50mg/L时,叶柄分化率明显变化,当浓度达到100mg/L时,分化率为1.4%。当壮观霉素浓度为40mg/L和50mg/L时,分化率为5.7%和3.7%,当浓度为60mg/L时,基本无绿芽分化,统计学分析显示:壮观霉素的抑制作用要强于卡那霉素。结合实验结果讨论了这两种抗生素作为甜菜遗传转化研究的筛选标记的适宜浓度。 相似文献
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为了培育打顶后不长侧芽的烟草植株,通过同源克隆,在烟草(品种K326)中得到了与侧芽生长相关基因的1个片断,将该片段序列通过BLAST检索Genbank同源比对,发现该基因同番茄的LS基因有非常高的同源序列,另外与水稻的MOC基因、拟南芥的LAS基因也有一定的同源性,命名为TLR基因.将TLR基因阅读框内640 bp的正向和反向片断构建到RNAi载体pHANNIBAL内含子两侧,再经NotI酶切回收约4300 bp目的片段,并插入到双元载体质粒pART27中,成功构建了含TLR基因片段正反向重复序列的植物表达载体pHANNIB AL-TLR-P ART27.将pHANNIB AL-TLR-P ART27质粒导入根癌农杆菌LBA4404中并转化烟草叶片细胞,没有得到转基因再生植株,推测这与TLR基因表达被抑制有关. 相似文献
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农杆菌介导快速、高效获得转基因烟草纯合株系 总被引:1,自引:0,他引:1
利用植物表达载体PBI121空载体,对农杆菌介导法转化烟草技术体系进行了综合改进,探索激素配比、侵染时间、分化和生根培养阶段卡那霉素的筛选压对烟草(Nicotiana tabacum cv.Petit Havana SR1)植株再生和转化效率的影响;同时根据孟德尔显性性状杂合子自交后代遗传分离规律,在转基因株系后代扩繁时用卡那霉素筛选分离,可以在T2代较快得到纯合株系.本研究通过使用整个叶片浸染、高的卡那霉素筛选剂浓度、控制生长条件以及强化管理,极大地缩短了获得纯合株系的时间,确立了该烟草品种快速、高效获得大量转基因阳性植株及快速获得转基因纯合株系的转化体系,其转化周期可减少至5-6个月,转基因植株阳性率可达到90%,其中60%的转基因植株后代卡那霉素抗性性状分离比例接近3:1. 相似文献
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Hepatitis B virus is responsible for significant morbidity and mortality despite the availability of safe and effective injectable vaccines. Transgenic plants are a novel system for expression and oral delivery of subunit vaccine antigens. In this paper, plant expression vector pBIS2S, which contains the HBsAg gene with precursor sequence preS2, was reconstructed based on pBI121 and was introduced into tobacco by the co-cultivating leaf-section method via Agrobacterium tumefaciens LBA4404. After selection by kanamycin and confirmation by PCR and Southern blot, it was found that the objective fragment had integrated into the tobacco genome. ELISA analysis showed that the hepatitis B surface antigen (HbsAg) was expressed in transgenic tobacco plants. These results indicate that the production of HbsAg with PreS2 extended to food plants as a source of edible vaccines is possible. 相似文献
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基于根瘤农杆菌(Agrobacterium tumefaciens)介导的方法实现异养蛋白核小球藻快速遗传转化,并优化转化条件。结果表明,小球藻异养生长条件的碳氮组合配比为葡萄糖20 g/L和酵母粉2 g/L时,生长速率为自养的3~5倍;根瘤农杆菌GV3101和LBA4404均可成功实现对异养小球藻的遗传转化,且转化效率无显著性差异(P>0.05)。最优转化条件为根瘤农杆菌GV3101初始OD600 nm值= 0.8,小球藻(Chlorella pyrenoidosa)浓度为107个/mL,各取100 μL混合涂布在pH=5.4、乙酰丁香酮浓度为200 μmol/L的CM平板上,24 ℃避光培养40 h后转到筛选平板,仅2 d转化子便清晰可见,数目达88个/106微藻。转化过程整体耗时仅5 d,普遍比自养微藻转化时间缩短3倍以上,为小球藻代谢工程改造提供技术手段,同时为其他可异养培养微藻的短时高效遗传转化提供科学依据。 相似文献
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Supartana P Shimizu T Shioiri H Nogawa M Nozue M Kojima M 《Journal of Bioscience and Bioengineering》2005,100(4):391-397
Seeds of rice (Oryza sativa L. var. Koshihikari) were soaked in water for 2 d. Thereafter, the embryo containing an apical meristem was inoculated with Agrobacterium tumefaciens by piercing a site of the husk overlying the embryonic apical meristem with a needle that had been dipped in an A. tumefaciens inoculum. The inoculated seeds were then grown to maturation (T0 plants) and allowed to pollinate naturally to set seeds (T1 plants) in pots under nonsterile conditions. To examine the transformation by various means, three different strains of A. tumefaciens were used for transformation: an M-21 mutant, which is an avirulent mutant with a Tn5 insertion in the iaaM gene, and two LBA4404 strains each with a different binary vector. Five different lines of evidence were demonstrated the transformation: the altered phenotype and its inheritance by the next generation, histochemical detection of beta-glucuronidase, resistance to hygromycin B, detection of the transgene by PCR and rescue of a plasmid consisting of the integrated T-DNA and the flanking rice genome DNA. Transformation efficiency of T1 plants was estimated to be 40% and 43% by PCR and a histochemical assay of beta-glucuronidase, respectively. 相似文献
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从转几丁质酶基因烟草株系(NPTII基因是选择标记基因)对卡那霉素的抗性,及外源几丁质酶基因表达蛋白的活性(Western Blot检测)两个方面对转基因株系的遗传稳定性进行了分析研究。在卡那霉素抗性检测试验中,检测了13个T2转基因株系,其中9个株系被检测种子100%对卡那霉素(100 mg/L)具有抗性;T3和T4转基因株系各检测了10个,100%被检测种子在含卡那霉素100 mg/L的MS培养基上长成了烟苗。Western Blot检测了11个T2株系,检测结果表明,6个株系被检测植株100%含有外源几丁质酶蛋白,2个株系被检测植株80%含有外源几丁质酶蛋白,2个株系被检测植株50%含有外源几丁质酶蛋白,1个株系被检测植株19.05%含有外源几丁质酶蛋白。在温室内将转基因烟草与未转基因烟草密植在一起,种植2代,烟株开花期模仿自然风力对烟株吹风,进行了转基因烟草是否可以通过花粉进行基因漂移的研究。研究结果表明,在自然风媒条件下未发现转基因烟草可进行基因漂移。 相似文献
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Hepatitis B virus is responsible for significant morbidity and mortality despite the availability of safe and effective injectable vaccines. Transgenic plants are a novel system for expression and oral delivery of subunit vaccine antigens. In this paper, plant expression vector pBIS2S, which contains the HBsAg gene with precursor sequence preS2, was reconstructed based on pBI121 and was introduced into tobacco by the co-cultivating leaf-section method via Agrobacterium tumefaciens LBA4404. After selection by kanamycin and confirmation by PCR and Southern blot, it was found that the objective fragment had integrated into the tobacco genome. ELISA analysis showed that the hepatitis B surface antigen (HbsAg) was expressed in transgenic tobacco plants. These results indicate that the production of HbsAg with PreS2 extended to food plants as a source of edible vaccines is possible. 相似文献
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半嵌套式PCR检测转几丁质酶基因烟草研究 总被引:5,自引:1,他引:4
用含卡那霉素的培养基首先对转几丁质酶基因烟草种子和烟苗进行初步筛选鉴定,再用Western Blot进一步证实抗卡那霉素的转基因烟苗中外源转几丁质酶基因的表达。然后研究半嵌套式PCR(Semi-nested PCR)检测转几丁质酶基因烟草植株及其烤后烟叶中的所转目的基因;利用限制性内切酶Hind Ⅲ对半嵌套式PCR产物进行酶切,从而验证半嵌套式PCR产物是否为真正的目的基因。研究结果表明,半嵌套式PCR是一种快速、灵敏的检测转几丁质酶基因烟草植株及烤后烟叶的方法。 相似文献