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Theodoros Eleftheriadis Georgios Pissas Marta Crespo Evdokia Nikolaou Vassilios Liakopoulos Ioannis Stefanidis 《International journal of molecular sciences》2021,22(4)
Direct allorecognition is the earliest and most potent immune response against a kidney allograft. Currently, it is thought that passenger donor professional antigen-presenting cells (APCs) are responsible. Further, many studies support that graft ischemia-reperfusion injury increases the probability of acute rejection. We evaluated the possible role of primary human proximal renal tubular epithelial cells (RPTECs) in direct allorecognition by CD4+ T-cells and the effect of anoxia-reoxygenation. In cell culture, we detected that RPTECs express all the required molecules for CD4+ T-cell activation (HLA-DR, CD80, and ICAM-1). Anoxia-reoxygenation decreased HLA-DR and CD80 but increased ICAM-1. Following this, RPTECs were co-cultured with alloreactive CD4+ T-cells. In T-cells, zeta chain phosphorylation and c-Myc increased, indicating activation of T-cell receptor and co-stimulation signal transduction pathways, respectively. T-cell proliferation assessed with bromodeoxyuridine assay and with the marker Ki-67 increased. Previous culture of RPTECs under anoxia raised all the above parameters in T-cells. FOXP3 remained unaffected in all cases, signifying that proliferating T-cells were not differentiated towards a regulatory phenotype. Our results support that direct allorecognition may be mediated by RPTECs even in the absence of donor-derived professional APCs. Also, ischemia-reperfusion injury of the graft may enhance the above capacity of RPTECs, increasing the possibility of acute rejection. 相似文献
3.
Abhendra K. Singh Kaitlyn Kahle Hannah James Allison L. Horner Daniel Villaflor Zach Benedict 《Ceramics International》2021,47(12):17268-17275
In this study the effects of high temperature and moisture on the impact damage resistance and mechanical strength of Nextel 610/alumina silicate ceramic matrix composites were experimentally evaluated. Composite laminates were exposed to either a 1050°C isothermal furnace-based environment for 30 consecutive days at 6 h a day, or 95% relative humidity environment for 13 consecutive days at 67°C. Low velocity impact, tensile and short beam strength tests were performed on both ambient and environmentally conditioned laminates and damage was characterized using a combination of non-destructive and destructive techniques. High temperature and humidity environmental exposure adversely affected the impact resistance of the composite laminates. For all the environments, planar internal damage area was greater than the back side dent area, which in turn was greater than the impactor side dent area. Evidence of environmental embrittlement through a stiffer tensile response was noted for the high temperature exposed laminates while the short beam strength tests showed greater propensity for interlaminar shear failure in the moisture exposed laminates. Destructive evaluations exposed larger, more pronounced delaminations in the environmentally conditioned laminates in comparison to the ambient ones. External damage metrics of the impactor side dent depth and area directly influenced the post-impact tensile strength of the laminates while no such trend between internal damage area and residual strength could be ascertained. 相似文献
4.
《International Journal of Hydrogen Energy》2022,47(8):5638-5649
The sustainable reduction of greenhouse gas emissions from road transport requires solutions to achieve net-zero carbon dioxide emissions. Therefore, in addition to vehicles with electrified powertrains, such as those implemented in battery electric of fuel cell vehicles, internal combustion engines fueled with e-fuels or biofuels are also under discussion. An e-fuel that has come into focus recently, is hydrogen due to its potential to achieve zero tank-to-wheel and well-to-wheel carbon dioxide emissions when the electrolysis is powered by electricity from renewable sources. Due to the high laminar burning velocity, hydrogen has the potential for engine operation with high cylinder charge dilution by e.g. external exhaust gas recirculation or enleanment, resulting in increased efficiency. On the other hand, the high burning velocity and high adiabatic flame temperatures pose a challenge for engine cooling due to increased heat losses compared to conventional fuels. To further evaluate the use of hydrogen for small passenger car engines, a series production 1 L 3 cylinder gasoline engine provided by Ford Werke GmbH was modified for hydrogen direct injection. The engine was equipped with a high pressure external exhaust gas recirculation system to investigate charge dilution at stoichiometric operation. Due to limitations of the turbocharging system, very lean operation, which can achieve nitrogen oxides raw emissions below 10 ppm, was limited to part load operation below BMEP = 8 bar. Thus, a reduction of the nitrogen oxides emission level at high loads compared to stoichiometric operation was not possible. At stoichiometric operation with external exhaust gas recirculation engine efficiency can be increased significantly. The comparison of stoichiometric hydrogen and gasoline operation shows a reduced indicated efficiency with hydrogen with significant faster combustion of hydrogen at comparable centers of combustion. However, higher boost pressures would allow to achieve even higher indicated efficiencies by charge dilution compared to gasoline engine operation. 相似文献
5.
Mit Rita Goswami Prayas Singh Pankaj Chamoli Sumit Bhardwaj Kuldeep Kumar Raina Ravi Kumar Shukla 《应用聚合物科学杂志》2021,138(43):51260
ZnO rice like nonarchitects are grafted on the graphene carbon core via a rapid microwave synthesis route. The prepared grafted systems are characterized via XRD, SEM, RAMAN, and XPS to examined the structural and morphological parameters. Zinc oxide grafted graphene sheets (ZnO-G) are further doped in β-phase of polyvinylidene fluoride (PVDF) to prepare the polymer nanocomposites (PNCs) via mixed solvent approach (THF/DMF). β-phase confirmation of PVDF PNCs is done by FTIR studies. It is observed that ZnO-G filler enhances the β-phase content in the PNCs. Non-doped PVDF and PNCs are further studied for rheological behavior under the shear rate of 1–100 s−1. Doping of ZnO-G dopant to the PVDF matrix changes its discontinuous shear thickening (DST) behavior to continues shear thickening behavior (CST). Hydrocluster formation and their interaction with the dopant could be the reason for this striking DST to CST behavioral change. Strain amplitude sweep (10−3% -10%) oscillatory test reveals that the PNCs shows extended linear viscoelastic region with high elastic modulus and lower viscous modulus. Effective shear thickening behavior and strong elastic strength of these PNCs present their candidature for various fields including mechanical and soft body armor applications. 相似文献
6.
为研究钢管套筒灌浆连接轴向受拉破坏过程及破坏机理,试验中设计了16组48个钢管套筒灌浆连接试件,试件采用钢板代替圆钢管,并进行静载试验。分析了灌浆料裂缝扩展过程、荷载-相对位移曲线,并对抗剪键高距比、灌浆料厚度、侧向力等因素对破坏过程及承载力的影响进行分析。结果表明:对于不设置抗剪键的套筒灌浆连接试件,斜裂缝随机产生,裂缝分布不均匀;对于设置抗剪键的套筒灌浆连接试件,裂缝首先出现在底部抗剪键位置处,与水平方向夹角约为30°,随后在中部和上部抗剪键位置处分别出现斜裂缝。由于每个抗剪键上荷载分担并不均匀,与抗剪键接触的灌浆料逐渐达到极限压应力,达到极限状态时,承载力全部由抗剪键间的机械咬合力承担,在连接承载力中,可忽略摩擦力和胶结力作用。随着抗剪键高距比h/s增大,各试件初始剪切刚度相差不大,承载力增大,但增幅逐渐减小,建议抗剪键高距比0.06g/s>0.3,同时需要满足灌浆料灌注的施工要求。 相似文献
7.
ABSTRACT This study investigates the effects of strain, strain rates, and forming directions (RD-rolling direction, TD-transverse direction, and ND-normal direction) on adiabatic shear, via dynamic impact compression tests using the Split Hopkinson Pressure Bar (SHPB) apparatus. A modified Johnson-Cook (J-C) constitutive model is proposed, which used to analyse the influence of the constitutive parameters on the sensitivity of adiabatic shear, employing a finite element software. The different sensitivities of adiabatic shear under different directions are explained by combining microscopic analysis and results from mechanical responses. The results show that the sensitivity of adiabatic shear can be related to the time of stress collapse in the following trend: ND?>?TD?>?RD; the sensitivities of these constitutive parameters on adiabatic shear are calculated and compared. 相似文献
8.
段潇乐 《湖南工业职业技术学院学报》2015,(1):82-84
本研究检测了直接和间接两种修正性反馈与国内高职理科学生写作水平的关系。本实验持续五周,将150名学习者分为直接反馈组、间接反馈组及控制组,共安排了一次预先测试,,一次即时后测和一次延时后测,并分别对各组进行考察,结果显示两个实验组进步显著,且直接反馈效果最明显。 相似文献
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10.
In this paper, we first re-examine the previous protocol of controlled quantum secure direct communication of Zhang et al.’s scheme, which was found insecure under two kinds of attacks, fake entangled particles attack and disentanglement attack. Then, by changing the party of the preparation of cluster states and using unitary operations, we present an improved protocol which can avoid these two kinds of attacks. Moreover, the protocol is proposed using the three-qubit partially entangled set of states. It is more efficient by only using three particles rather than four or even more to transmit one bit secret information. Given our using state is much easier to prepare for multiqubit states and our protocol needs less measurement resource, it makes this protocol more convenient from an applied point of view. 相似文献