Improved aqueous solubility,bioaccessibility and cellular uptake of quercetin following pH-driven encapsulation in whey protein isolate

The purpose of this study was to increase the water solubility and potential bioavailability of quercetin by encapsulation in whey protein isolate (WPI) based on a green, efficient pH-driven method. According to the results, the water solubility of quercetin increased by 346.9: times after loading into WPI nanoparticles. When the initial quercetin concentration was 0.25 mg mL⁻¹ and WPI was 2% w/v, the encapsulation efficiency reached 94.1%, the Z-average diameter was 36.63 nm, and the zeta potential was −36.4 mV at pH 7.0. The fluorescence spectroscopy assay suggested the molecular complexation of quercetin and WPI at pH 12.0. X-ray diffraction assay indicated the enclosure of amorphous quercetin in WPI. Correspondingly, the bioaccessibility increased from 2.76% to 31.23% and the Caco-2 cell monolayer uptake increased from 0% to 2.12% after nanoencapsulation. This work confirmed that the pH-driven method is an effective approach to prepare WPI–quercetin nanocapsules to improve physical and potentially biological properties of quercetin.     

基于人文关怀的信号配时方法研究

在传统的信号配时中，都是以减少车辆的总延误为目标，这样并不能很好地体现出整个社会中人的时间价值。现以减少所有通过交叉口的出行者的总延误为目标，在传统的信号配时方案的基础上，提出了一套新的配时方案，同时以减少全部出行者的总延误时间为目标给出了相应的延误评价公式，最后以杭州市文一路-古翠路口的实测交通数据进行分析，论证了本配时方案的有效性。     

Fractionation and characterisation for antioxidant activity of hydrolysed whey protein

Whey protein isolate (WPI) was hydrolysed for 1 h using Alcalase, Protamex and Flavourzyme. Native WPI, hydrolysed WPI and two commercial WPI hydrolysates were subjected to fractionation by size exclusion chromatography. Antioxidant activity of WPI fractions was measured with a liposome‐oxidising system (50 µM FeCl₃/0.1 µM ascorbate, pH 7.0). Lipid oxidation was measured as thiobarbituric acid‐reactive substances (TBARS). Gel electrophoresis and amino acid analysis were run to identify the peptide composition. The influence of amino acid composition on antioxidant activity was evaluated using multivariate analysis methods (correlation analysis, principal component analysis, multiple linear regression and discriminant analysis). TBARS assays indicated the presence of antioxidant activity in all protein fractions, including non‐hydrolysed WPI. For native and hydrolysed WPI samples the first fraction (> 45 kDa) showed a higher TBARS inhibition effect (24–27%) when compared with lower‐molecular‐weight fractions and hydrolysate mixtures. In contrast, for commercial WPI hydrolysates a higher inhibitory effect was found in most of the lower‐molecular‐weight fractions (30–55%). The ability of WPI fractions to delay lipid oxidation was found to be related to the prevalence of histidine and hydrophobic amino acids. Copyright © 2004 Society of Chemical Industry     

The Horace Method for Error-Correcting Codes

Here we apply the so-called Horace method for zero-dimensional schemes to error-correcting codes on complete intersections. In particular, we obtain sharper estimates on the minimum distance.     

折线链节点标注技术

针对传统多边形位置关系计算比较烦琐,以及简单多边形的理论难以拓展到一般多边形的问题,提出标注节点状态的方法.通过定义11种位置来描述折线链上每个节点的状态,再采用"线段端点与线段"和"线段端点与邻折线"的标注方法来实现任意折线链的标注,同时利用两线段分割预处理使相交仅发生在端点处,从而使算法更高效;然后给出折线链基本位置关系的节点特征,并且探讨了三维顶点的标注方法.该方法的标注原理简单、方法实用,算法空间和时间复杂度分别为O(n)和O(n2).实验结果表明,该方法对任意形状的折线链都能实现稳定标注;通过搜索节点状态特征可以求解折线链间的相互关系,还可以实现一般折线链的碰撞检测、相交区域计算以及多边形简单化分解等.     

动态优化不等精度经纬仪交会方法

在多台不等精度经纬仪交会测量系统中,为提高数据的可靠性和精度,根据异面交会原理,提出空间定位精度及其误差的模型;提出采用定常异动算子算法和普通加权平均法相结合的处理方法;对该算法进行数学仿真,计算分别使用定常异动算子和普通加权平均法的交会角区间,并在实际任务中加以应用。试验结果表明,采用合理的算法对多台不等精度经纬仪进行交会测量,增加了测量基线数量从而提高了整体数据的稳定性,同时提高了测量精度。     

带观测滞后和不确定噪声方差的多智能体传感网络鲁棒序贯协方差交叉融合Kalman滤波

针对带观测滞后和不确定噪声方差的分簇多智能体传感网络系统,研究鲁棒序贯协方差交叉融合Kalman滤波器的设计问题.应用最邻近法则,传感网络被分成簇.应用极大极小鲁棒估计原理,基于带噪声方差最差保守上界的最差保守传感网络系统,提出了两级序贯协方差交叉(SCI)融合鲁棒稳态Kalman滤波器,可减小通信和计算负担并节省能量,且保证实际滤波误差方差有一个最小保守上界.一种Lyapunov方程方法被提出用于证明局部和融合滤波器的鲁棒性.提出了鲁棒精度的概念且证明了局部和融合鲁棒Kalman滤波器的鲁棒精度关系.证明全局SCI融合器的鲁棒精度高于每簇SCI融合器的精度且两者的鲁棒精度都高于每个局部鲁棒滤波器的精度.一个跟踪系统的仿真例子证明了鲁棒性和鲁棒精度关系.