南美白对虾体内氧氟沙星的检测方法的研究

确定了一种检测对虾体内氧氟沙星含量的方法——反相高效液相色谱法(RP-HPLC)，氧氟沙星在0.26～32．0mg／L质量浓度范围内的线性关系（γ=0．9998)。并利用此方法研究了氧氟沙星在南美白对虾体内的分布，结果为氧氟沙星能够分布在对虾的肝胰腺、血淋巴、鳃、肌肉。     

消旋氧氟沙星抗体制备及其手性识别特性研究

采用活泼酯法（A）和直接EDC（1-乙基-3-（3-二甲氨丙基）碳二亚胺盐酸盐）法（D）,合成两种包被抗原（OFL-A-OVA和OFL-D-OVA）和两种免疫抗原（OFL-A-BSA和OFL-D-BSA）,然后分别用两种免疫抗原免疫小鼠获得与其对应的多克隆抗体,建立消旋氧氟沙星残留免疫检测方法。研究结果表明：紫外扫描图谱证明四种抗原均合成成功,OFL-A-BSA、OFL-D-BSA、OFL-A-OVA和OFL-D-OVA的偶联比分别为19.67、3.03、1.93和0.99。消旋氧氟沙星的间接竞争ELISA法的线性检测范围为16.35～444.10ng/mL,IC50为5.42ng/mL,最低检测限为6.23ng/mL。消旋氧氟沙星抗体与恩诺沙星、环丙沙星、加替沙星等其他同类药物的交叉反应率较低,表明消旋氧氟沙星抗体具有较高的特异性,且消旋氧氟沙星抗体对左旋氧氟沙星和右旋氧氟沙星的识别并非对等,对左旋的识别能力较大,左旋氧氟沙星对抗体的产生起主要作用。     

氧氟沙星-壳聚糖-明胶共混膜的制备及表征

制备了壳聚糖 明胶共混膜 ,测定了壳聚糖 明胶共混膜的抗张强度 ,并以抗张强度最大的壳聚糖 明胶共混膜为载体 ,氧氟沙星为模型药物制得具有抗菌性能的氧氟沙星 -壳聚糖 -明胶共混膜。通过红外光谱 (FT -IR) ,X射线衍射 (X ray) ,扫描电子显微镜 (SEM)表征了共混膜的特性。结果表明 :明胶质量分数为 2 5 %时 ,壳聚糖 明胶共混膜的抗张强度最大 ,达 5 5MPa。壳聚糖、明胶、氧氟沙星三者在共混膜中有很好的相容性 ,并且形成了分子间氢键     

Preparation,Antibacterial and Physicochemical Behavior of Chitosan/Ofloxacin Complexes

A novel chitosan derivative with ofloxacin (OFX) has been successfully prepared. The IR and 1H-NMR results revealed that the chitosan/ofloxacin (CH-OFX) complex exhibited an electrostatic interaction. The crystalline and surface morphology were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The antimicrobial activity of the complexes against various micro-organisms viz. Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus was tested. It was established that their antibacterial activity is up to four times greater than that of free quinolone drug and chitosan, probably due to the conjunction of favorable pharmacokinetics, excellent bacterial susceptibility and good stability towards metabolic degradation.     

原料乳中氧氟沙星残留量对S. thermophilus grx02发酵乳质构及流变特性的影响

选择对氧氟沙星敏感的嗜热链球菌（S. thermophilus）grx02作为发酵微生物,研究了不同氧氟沙星残留量对其生长曲线及发酵乳质构和流变特性的影响。结果表明：当原料乳中氧氟沙星残留量达到1.0 μg/mL时,会使S. thermophilus grx02的生长速率、发酵乳的黏附性、胶黏性和咀嚼性显著降低;当氧氟沙星残留量达到0.5 μg/mL时,会对S. thermophilus grx02发酵乳的流变特性产生显著影响,使其凝胶稳定性降低,抗机械运动和温度变化的能力降低（P＜0.05）。上述结果表明,用于发酵乳制品生产的原料乳中氧氟沙星的残留限量应该控制在0.5 μg/mL以下。     

Synthesis and characterization of chitosan/cloisite 30B film for controlled release of ofloxacin

In this research program, chitosan film was prepared by blending chitosan with Cloisite 30 B at different concentrations 0 wt %, 1 wt %, and 2.5 wt %. The blends were characterized by Fourier transmission infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X‐ray diffraction (XRD) analysis. From the FTIR spectra the various groups present in chitosan/C 30 B blend were monitored. The homogeneity, morphology, and crystallinity of the blends were ascertained from SEM and XRD data, respectively. The most suitable form of blend was taken and used as a carrier for the controlled release of ofloxacin. The swelling studies have been carried out at different drug loading. Drug release kinetics was analyzed by plotting the cumulative release data versus time by fitting to an exponential equation which indicated the occurrence of non‐Fickian type of kinetics. The drug release was investigated at different pH medium and it was found that the drug release depends upon the pH medium as well as the nature of matrix. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012     

荧光光度法测定鱼肉中的氧氟沙星残留

采用荧光光度法测定了鲫鱼、鳗鱼、鲤鱼和罗非鱼肌肉组织中的氧氟沙星药物残留量。样品用酸性乙腈提取,正己烷脱脂,水相在B-R(pH4.0)缓冲溶液中,激发波长λex294nm,荧光波长λem496nm下用荧光光度法测定其中的氧氟沙星含量,氧氟沙星的线性范围0.03～0.20μg/ml,相关系数r=0.9929,检出限为0.005μg/ml。回收率为76.0%～94.5%。日内变异系数为2.14%～6.50%,日间变异系数为3.02%～9.78%。     

The effect of thiol content on the gelation and mucoadhesion of thiolated poly(aspartic acid)

A synthetic poly(amino acid), poly(aspartic acid) modified with thioethyl side groups, was studied as an in situ gelling and mucoadhesive dosage form. The chemical structure and the controllable, large thiol content of the polymer were confirmed using ¹H NMR spectroscopy and Ellman's assay. In situ gelation of aqueous polymer solutions was induced by oxidation and monitored using oscillation rheometry. The strength of mucoadhesion towards excised mucosa was characterised by tensile tests in ex vivo experiments. Release kinetics of ophthalmic antibiotic ofloxacin was studied from an in situ crosslinked hydrogel and a liquid formulation to prove the sustained release of the former. © 2017 Society of Chemical Industry