直接法合成月桂酰基甲基牛磺酸钠

以月桂酸和N-甲基牛磺酸钠为原料,经直接缩合一步反应合成了月桂酰基甲基牛磺酸钠。考察了直接法缩合工艺中反应温度、反应时间、催化剂种类和用量、投料比、溶剂用量等对反应的影响。结果表明,优化反应条件为:月桂酸与N-甲基牛磺酸钠投料摩尔比为1.3∶1,催化剂硼酸用量为月桂酸质量的2%,溶剂用量为月桂酸质量的40%,215℃反应6 h。在上述条件下,N-甲基牛磺酸钠的转化率可达95%以上,粗产品经重结晶及干燥处理后纯度可达98%以上。     

The methionine precursor dl-2-hydroxy-(4-methylthio)butanoic acid protects intestinal epithelial barrier function

dl-2-hydroxy-(4-methylthio)butanoic acid (HMTBA) is a source of dietary methionine (Met) that is widely used in poultry nutrition. We have previously shown that HMTBA is preferentially diverted to the transsulfuration pathway, which gives antioxidant metabolites such as taurine and glutathione. Therefore, here we hypothesize that this Met source can protect epithelial barrier function in an in vitro model of intestinal inflammation of Caco-2 cells. The results show that HMTBA prevents the increase in paracellular permeability induced by H₂O₂ or tumour necrosis factor-α. This effect can be attributed to the increased production of taurine and reduced glutathione. Similar results were obtained for dl-Met, although the protective role of the amino acid was less pronounced than that of the hydroxy analogue. In conclusion, the diversion to the transsulfuration pathway means that this Met precursor is of greater value than previously thought, due to its capacity to improve intestinal homeostasis and the quality of poultry products destined for human consumption.     

离子交换法分离牛磺酸与硫酸钠的研究

牛磺酸 (taurine)具有消炎 ,解热镇痛和促进大脑发育、增强视力、调节神经传导及促进吸收等作用。作为添加剂 ,已广泛用于药物、食品饮料中 ,具有进一步推广使用的前景。但药用和化学用的大量牛磺酸主要通过化学合成法生产 ,最终反应产物为牛磺酸和硫酸钠的混和物 ,二者的分离方法将是牛磺酸纯度和得率的关键。本文探讨了不同的离子交换树脂和操作条件对分离的影响 ,取得了满意的结果。用离子交换法来分离牛磺酸和硫酸钠 ,阳树脂为 0 0 1× 7,阴树脂为D30 1 -SC ,交换柱 2 5× 60 0mm ,阴阳树脂体积比为 1 1 .37,试液浓度 0 .4mol·L- 1 ,流速 5 .0ml·min- 1 ,牛磺酸回收率为 97.0 % ,纯度 99.5 %。     

2-氨基乙磺酸钠-4,6-二氯-1,3,5-均三嗪的合成

以三聚氯氰和氨基乙磺酸为原料,在0℃~5℃下进行反应,以薄层色谱法确定其反应终点,最终制得了一种新型的阴离子磺酸型表面活性剂2-氨基乙磺酸钠-4,6-二氯-1,3,5-均三嗪,并用红外光谱、1HNMR谱、13CNMR谱对其进行结构验证。     

荧光法测定啤酒中牛磺酸含量

以啤酒为基质,牛磺酸为添加剂,采用强酸性阳离子交换柱分离出牛磺酸,与邻苯二甲醛(OPA)/2-巯基乙醇衍生化后进行荧光法测定。结果表明:相对标准偏差≤2.4%,回收率>95%,最小检出限为0.023μg/mL;放置6个月的和现配的样品中牛磺酸含量差别无显著性(p>0.05)。该方法快速、简便而且重现性好,可用于测定啤酒中添加的牛磺酸。     

Taurine ameliorates alcoholic steatohepatitis via enhancing self-antioxidant capacity and alcohol metabolism

Chronic alcohol consumption or alcohol abuse is the main cause of alcoholic steatohepatitis or further cirrhosis. This study was to exam if the antioxidant capacity and alcohol metabolism in livers of chronic alcohol-fed rats were improved by supplementing taurine (Tau). Rats were randomly divided into four groups with five times per week of treatment: 1) isocaloric solution; 2) 3 g alcohol/kg BW/day; 3) 3 g alcohol/kg BW/day + 1 g taurine (Tau)/kg BW/day; and 4) 3 g alcohol/kg BW/day + 2 g Tau/kg BW/day. A 6-week alcohol consumption resulted in lower (p < 0.05) body weight gain and self-antioxidant capacities, as well as increased (p < 0.05) liver size, serum/hepatic lipids, and AST and ALT values. However, alcohol-fed rats co-treated with Tau have decreased (p < 0.05) liver lipid levels via increasing fecal lipid output and cholesterol metabolism. Besides, co-treatment of Tau also enhanced (p < 0.05) self-antioxidant capacities and alcohol metabolism in livers via enhancing GSH contents, CAT, GSH-Px, ADH, and ALDH activities, but decreasing MDA contents. In a histological examination of rat liver, microvesicular steatosis and necrotic cells were observed in alcohol-fed rats without Tau while largely suppressed microvesicular steatosis and no necrotic cells were observed in alcohol-fed rat supplemented with Tau. Therefore, Tau could be an effective hepatoprotective agent against alcohol-induced damage via enhancing self-antioxidant capacity and alcohol metabolism.     

牛磺酸功能啤酒抗疲劳作用研究

目的:研究牛磺酸功能啤酒对小鼠的抗疲劳效果。方法:小鼠随机分成对照组和高、中、低3个剂量组,剂量分别为10g/L、5g/L、2.5g/L,对照组给予等剂量空白啤酒灌胃,共30d。测定负重游泳时间、肝糖原(LG)、尿素氮(BUN)和血乳酸(LAC)指标。结果:3个剂量组均能显著提高小鼠运动耐力以及LG的储备量;能显著降低运动后LAC和BUN的含量。结论:牛磺酸功能啤酒具有良好的抗运动疲劳作用。     

牛磺酸对半乳糖性白内障大鼠晶状体超微结构的影响

目的：观察牛磺酸在实验性半乳糖性白内障发生发展过程中对晶状体超微结构的影响。方法：应用冷冻复型，超薄切片透射电镜技术对半乳糖性人障大鼠晶状体及给予牛磺酸后大鼠晶状体超微结构的改变进行了观察。结果：半乳糖性白内障大鼠晶状体纤维表面光滑，膜内颗粒减少，未见典型缝管连接，晶状体纤维细胞间隙协大，细胞质内充满大小不等的空泡状结构，牛磺酸投药后晶状依纤维细胞膜内颗粒增多，可见典型缝管连接，晶状体纤维细胞胞质     

牛磺酸的合成

以乙醇胺为原料用硫酸酯化和亚硫酸钠磺化的路线合成牛磺酸,对第二步磺化反应进行了专题实验,通过改变工艺条件和操作方法,使磺化反应得到了较高的产率,同时验证了影响产率的主要因素,并在反应机理上进行阐述.