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101.
Previously, we showed that the N-terminal recognition domain (T1) of Kv1.3 was not required for assembly of functional channels [Tu et al. (1996) J. Biol. Chem. 271, 18904-18911]. Moreover, specific Kv1.3 peptide fragments including regions of the central core are able to inhibit expression of current produced from a channel lacking the T1 domain, Kv1.3(T1-). To elucidate the mechanism whereby Kv1.3 peptide fragments suppress Kv1.3(T1-) current, we have studied the ability of peptide fragments containing the transmembrane segments S1, S1-S2, or S1-S2-S3 to physically associate with the Kv1.3(T1-) polypeptide subunit in vitro in microsomal membranes. Using c-myc (9E10) epitope-labeled peptide fragments and anti-myc antibody as well as antisera to the Kv1.3 C-terminus, we now demonstrate specific association of these peptide fragments with Kv1.3(T1-). Association of peptide fragments with Kv1.3(T1-) was correlated with integration of both proteins into the membrane. Furthermore, the relative strength and kinetics of this association directly correlated with the ability of fragments to suppress Kv1.3(T1-) current. The rate-limiting step in the sequential synthesis, integration, and formation of a complex was the association of integrated polypeptides within the plane of the lipid bilayer. These results strongly suggest that the physical association of transmembrane segments provides the basis for suppression of K+ channel function by K+ channel peptide fragments in vivo. Moreover, the S1-S2-S3 peptide fragment potently suppressed full-length Kv1.3, thus implicating a role for the S1-S2-S3 region of Kv1.3 in the assembly of the Kv1.3 channel. We refer to these putative association sites as IMA (intramembrane association) sites. 相似文献
102.
N-羟甲基丙烯酰胺的合成 总被引:6,自引:0,他引:6
对N-羟甲基丙烯酰胺(NAM)的合成工艺做了进一步的研究。结果表明:在丙烯酰胺:甲醛=1∶1.05(mol比),碱性催化剂存在下,70℃反应3h。转化率可达99%。产品质量稳定,反应液可不经分离直接用作交联剂,降低了生产成本。并提出用固含量法测定溶液中的NAM含量,简易可靠。 相似文献
103.
104.
105.
油菜素内脂(BR)与烯效唑(S3307)复合剂处理黄瓜幼苗提高了叶片的叶绿素含量。光合速率,SOD酶活性和MDA含量降低,相对电解质渗透显降低,幼苗的根系活力显提高。田间试验结果表明,BS处理比对照增产11.62%,瓜数增加17.72%,因此,BR和SS3307复合剂在生产上有一定的应用推广价值。 相似文献
106.
107.
Due to patient demand for biocompatible, safe, and tooth-colored restorations, the use of amalgam as a restorative material has been declining. Currently, the profession of operative dentistry is in a state of transition from the amalgam age to the post-amalgam age. The learning objective of this article is to discuss the current state of the art of tooth-colored restoratives--amalgam substitutes and amalgam alternatives--used either as direct or laboratory fabricated restorations nationally and internationally. Adhesive dentistry is discussed in detail, with tooth preparation as the key element. The challenges facing the clinician during the transition period include clinician education, restorative concepts and teaching competence, costs, prevention, and maintenance. 相似文献
108.
抗高温磨损高铬铸铁的研究 总被引:3,自引:0,他引:3
经Mo、B、V、Ti合金化和RE变质处理的高铬铸铁,具有优良的抗高温磨损性能和强韧性,用于制造筛分高温烧结矿的振动筛筛板,使用寿命比ZG30Cr18Mn12Si2N耐热钢筛板提高2倍以上。多元高铬铸铁筛板制造工艺简单,成本低,值得在筛分领域推广使用。 相似文献
109.
Microfibre reinforced cement composites reinforced with high volume fractions of carbon, steel and polypropylene fibres were tested in uniaxial tension. Composites investigated included those with only one type of fibre (mono-fibre composites) and those with two or more types of fibres (hybrid-fibre composites) in the same mix. Considerable strengthening, toughening and stiffening of the host matrix due to microfibre reinforcement were observed. In the hybrid-fibre composites, different fibres appear to act as additive phases; i.e., they maintain their individual reinforcing capabilities. The composites were also impact tested in uniaxial tension using a newly designed instrumented impact machine. When compared with static test results, considerable sensitivity to stress rate was noted; composites were found to be stronger and tougher under impact and the improvements were more pronounced at higher fibre volume fractions. The potential of these composites for use in thin sheet products and other similar applications is recognized, and the need for continued research is stressed. 相似文献
110.
马来酸酐在乙丙橡胶上的接枝共聚 总被引:4,自引:0,他引:4
马来酸酐在乙丙橡胶上的接枝共聚,用过氧化苯甲酰为引发剂的效果优于偶氮二异丁腈。研究了温度、马来酸酐用量和引发剂用量等对接枝率的影响。用红外光谱和薄层色谱证明了接枝物的存在。用接触角技术测定了接枝物的表面性能。 相似文献