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排序方式: 共有1215条查询结果,搜索用时 15 毫秒
61.
Keiichi Miyamoto Ayaka Kawasaki Yuko Nagata Masanori Uraya Hisayoshi Kojima Takanori Ito Takashi Horiuchi Nagisa Asakawa Shinsuke Nomura 《Materials science & engineering. C, Materials for biological applications》2011,31(2):158-165
Immunoglobulin A1 (IgA1) concentration in the plasma of patients with IgA nephropathy (IgAN) as the cause of renal failure is higher than that in the plasma of normal controls. IgA1 with abnormal sugars is considered to deposit in the glomerular mesangium, aggravating nephritis in IgAN. Jacalin is a lectin that recognizes sugars on IgA1. However, its selective-recognition for normal-type (ST type, NeuAc-α(2,3)-Gal-β(1,3)-GalNAc) and abnormal-type (T type, Gal-β(1,3)-GalNAc; Tn type, GalNAc; STn type, NeuAc-α(2,6)-GalNAc) sugars α-O-linked to serine/threonine in IgA1 is weak. Therefore, jacalin cannot be used for recognizing specific sugar types on IgA1. We attempted to develop a new recognition method for specific sugar types on IgA1 by utilizing the multirecognition capability of jacalin. Its binding abilities were regulated by heat denaturation with suitable template sugar (galactose or N-acetylgalactosamine). Further, we successfully prepared denatured-jacalin derivatives, which recognized ST-/T-type sugars on IgA1, by sugar-immobilized affinity chromatography. Enzyme-linked immunosorbent assay of denatured-jacalin derivatives, showed the ratios of abnormal sugars on IgA1 in the plasma of IgAN patients and normal controls to be approximately 60% and 20%, respectively. The results proved that profiling of sugar types in IgAN can successfully be performed by solely using jacalin derivatives. 相似文献
62.
R. Kondo Y. Tsutsumi H. Doi N. Nomura T. Hanawa 《Materials science & engineering. C, Materials for biological applications》2011,31(5):900-905
The effects of Pt and Pd addition to a Zr-20Nb alloy on its microstructure and mechanical property, as well as the elution of metals from the alloys in lactic acid solution, were investigated. The microstructure was characterized with an X-ray diffractometer (XRD), an optical microscope (OM), and a transmission electron microscope (TEM). The mechanical properties were evaluated by a tensile test. The β phase is dominantly observed in the Zr-20Nb as well as in the Pt-added and Pd-added Zr-20Nb alloys. Needle-like microstructures are observed in equiaxed grains in all alloys. Pd addition to the Zr-20Nb alloy suppresses ω phase formation more than Pt addition does. The 0.2% offset yield strength and the ultimate tensile strength of the Pt-added and Pd-added Zr-20Nb alloys increase with the Pt and Pd concentrations. XRD analysis revealed that the lattice parameter of β-Zr in the Pt-added and Pd-added Zr-20Nb alloys decreases with the Pt and Pd concentrations. Pt and Pd solute in β-Zr as a substitutional element and contribute to the increase in the strength by solid solution hardening. The addition of 2Pt and 2Pd to the Zr-20Nb alloy also improves metal elution from the alloys in lactic acid solution. 相似文献
63.
Takuya Takahashi Motoya Suzuki Ryuji Nomura Yuichi Okuda Koji Kamiya Takenori Numazawa Peter Shirron 《Microgravity science and technology》2011,23(4):365-372
Superfluid 4He was produced on a small jet plane for the first time using a small GM-refrigerator to condense the liquid and a scroll
pump to get the superfluid by evaporation. The surface wave on superfluid under 0.5g
E, 0.1g
E and 0.05g
E, together with 2g
E and 1g
E, was successfully examined by an optical method utilizing parabolic flight. Here, g
E is the gravitational constant on the ground. Assuming that only the fundamental mode was excited as determined by the sample
cell width, the resonance peak in the frequency domain was well reproduced by the gravity wave with corresponding gravity
constant. 相似文献
64.
Weixiao Huang Chow T.P. Niiyama Y. Nomura T. Yoshida S. 《Electron Device Letters, IEEE》2009,30(10):1018-1020
We report on the experimental demonstration of a novel n-channel GaN epilayer RESURF GaN MOSFET with good tradeoff between breakdown voltage and specific on-resistance for the first time. Device with 4-mum channel length and 16-mum RESURF length has breakdown voltage up to 730 V with specific on-resistance 34 mOmegamiddotcm2 (VG - VT = 20 V), best reported to date. 相似文献
65.
Tsuyoshi Nomura Masahiro Ikeda Seiji Ishiyama Kazuhide Mita Toshiki Tamura Takahiro Okada Kazuhito Fujiyama Akihiro Usami 《Journal of Bioscience and Bioengineering》2010,110(4):386-391
In insects, β-N-acetylglucosaminidase (GlcNAcase) participates in critical physiological processes such as fertilization, metamorphosis, and glycoconjugate degradation. Insects produce glycoproteins carrying paucimannosidic-type N-glycans, the terminal GlcNAc residue of which is cleaved by a GlcNAc-linkage specific GlcNAcase, also known as the fused lobes (FDL) protein. To obtain information on the structure of GlcNAcases and insight into their contribution to physiological processes, we cloned Bombyx mori FDL (BmFDL) from silkworm larvae. The full-length cDNA (1.9 kb) encoded a protein of 633 amino acids with 42% amino acid sequence identity to Drosophila melanogaster FDL (DmFDL). Recombinant BmFDL cleaved only β-1,2-linked GlcNAc residues from the α-1,3 branch of biantennary N-glycan. This substrate specificity was similar to that of DmFDL. Microsomal FDL activity was inhibited by anti-BmFDL antibodies. Taken together, our results suggest that BmFDL is a N-glycan-processing GlcNAcase in B. mori. 相似文献
66.
Genki Kobayashi Shin‐ichi Nishimura Min‐Sik Park Ryoji Kanno Masatomo Yashima Takashi Ida Atsuo Yamada 《Advanced functional materials》2009,19(3):395-403
State‐of‐the‐art LiFePO4 technology has now opened the door for lithium ion batteries to take their place in large‐scale applications such as plug‐in hybrid vehicles. A high level of safety, significant cost reduction, and huge power generation are on the verge of being guaranteed for the most advanced energy storage system. The room‐temperature phase diagram is essential to understand the facile electrode reaction of LixFePO4 (0 < x < 1), but it has not been fully understood. Here, intermediate solid solution phases close to x = 0 and x = 1 have been isolated at room temperature. Size‐dependent modification of the phase diagram, as well as the systematic variation of lattice parameters inside the solid‐solution compositional domain closely related to the electrochemical redox potential, are demonstrated. These experimental results reveal that the excess capacity that has been observed above and below the two‐phase equilibrium potential is largely due to the bulk solid solution, and thus support the size‐dependent miscibility gap model. 相似文献
67.
The isotope effects of gadolinium in Gd-EDTA ligand exchange system were studied by means of ion exchange chromatography. The separation coefficients of gadolinium isotopes, epsilon, and the local enrichment factors, beta, were calculated from the observed isotopic ratios at the front and rear boundaries of the gadolinium adsorption band. Clear mass independent anomalies were observed in the isotope effects of 155Gd and 157Gd. The relation between the isotope effects of gadolinium isotopes, studied by the three-isotope plot and the separation coefficient methods, and the mass of gadolinium isotopes was found to be related to the change in the mean square radius of the nuclear charge distribution parameter, (r2), of these isotopes, which suggests that the nucleus shape and size highly affect the gadolinium isotope effects in chemical exchange reactions. 相似文献
68.
Interferograms obtained with ordinary interferometers, such as the Fizeau interferometer or the Twyman-Green interferometer, show the contour maps of a wave front under test. On the other hand, lateral shearing interferograms show the difference between a wave front under test and a sheared wave front, that is, the inclination of the wave front. Therefore the shape of the wave front under test is reconstructed by means of analyzing the difference. To reconstruct the wave front, many methods have been proposed. An integration method is usually used to reconstruct the wave front under test rapidly. However, this method has two disadvantages: The analysis accuracy of the method is low, and part of the wave front cannot be measured. To overcome these two problems, a new, to our knowledge, integration method, improved by use of polynomials, is proposed. The validity of the proposed method is evaluated by computer simulations. In the simulations the analysis accuracy achieved by the proposed method is compared with the analysis accuracy of the ordinary integration method and that of the method proposed by Rimmer and Wyant. The results of the simulations show that the analysis accuracy of the newly proposed method is better than that of the integration method and that of the Rimmer-Wyant method. 相似文献
69.
70.
Techniques for measuring aberrations in lenses used in photolithography with printed patterns 总被引:3,自引:0,他引:3
In optical lithography, it is a serious problem that aberrations in projection lenses reduce the imaging quality. Therefore techniques to measure the aberrations are required that will predict the adverse effects of aberrations on lithographic imagery and reduce them. We present a measurement method that uses a fine grating and its imaging condition to quantify coma, astigmatism, and spherical aberration. With this method, these aberrations can be described with simple expressions from the measured results. Application of this method revealed the coma of Zernike polynomials for our krypton fluoride (KrF) excimer-laser scanner. 相似文献