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排序方式: 共有313条查询结果,搜索用时 15 毫秒
1.
The emission rate of carbon dioxide (CO2) depends on many factors but mainly on the activity level (metabolic rate) of occupants. In this study, we examined two other factors that may influence the CO2 emission rate, namely the background CO2 concentration and the indoor temperature. Six male volunteers sat one by one in a 1.7 m3 chamber for 2.5 h and performed light office-type work under five different conditions with two temperature levels (23 vs. 28°C) and three background concentrations of CO2 (800 vs. 1400 vs. 3000 ppm). Background CO2 levels were increased either by dosing CO2 from a cylinder or by reducing the outdoor air supply rate. Physiological responses to warmth, added CO2, and bioeffluents were monitored. The rate of CO2 emission was estimated using a mass-balance equation. The results indicate a higher CO2 emission rate at the higher temperature, at which the subjects were warm, and a lower emission rate in all conditions in which the background CO2 concentration increased. Physiological measurements partially explained the present results but more measurements are needed. 相似文献
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Atsushi Kawano Kansuke Fukui Yuji Matsumoto Atsushi Terada Akihiro Tominaga Nozomi Nikaido Takashi Tonozuka Kazuhide Totani Nozomu Yasutake 《Journal of Applied Glycoscience》2020,67(2):41
According to whole-genome sequencing, Aspergillus niger produces multiple enzymes of glycoside hydrolases (GH) 31. Here we focus on a GH31 α-glucosidase, AgdB, from A. niger . AgdB has also previously been reported as being expressed in the yeast species, Pichia pastoris ; while the recombinant enzyme (rAgdB) has been shown to catalyze tranglycosylation via a complex mechanism. We constructed an expression system for A. niger AgdB using Aspergillus nidulans . To better elucidate the complicated mechanism employed by AgdB for transglucosylation, we also established a method to quantify glucosidic linkages in the transglucosylation products using 2D NMR spectroscopy. Results from the enzyme activity analysis indicated that the optimum temperature was 65 °C and optimum pH range was 6.0–7.0. Further, the NMR results showed that when maltose or maltopentaose served as the substrate, α-1,2-, α-1,3-, and small amount of α-1,1-β-linked oligosaccharides are present throughout the transglucosylation products of AgdB. These results suggest that AgdB is an α-glucosidase that serves as a transglucosylase capable of effectively producing oligosaccharides with α-1,2-, α-1,3-glucosidic linkages. 相似文献
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Anna Giovanna Sciancalepore Elisa Mele Valentina Arcadio Francesco Reddavide Francesco Grieco Giuseppe Spano Patrick Lucas Giovanni Mita Dario Pisignano 《Food microbiology》2013
The development of fast, reliable and culture-independent molecular tools to detect bacteria producing biogenic amines deserves the attention of research and ultimately of the food industry in order to protect consumers' health. Here we present the application of a simple, low-cost, fast and sensitive method to perform microdroplet-based multiplex PCR, directly on a food matrix, for the simultaneous detection of bacterial genes involved in biogenic amine biosynthesis. After inoculating wine with Lactobacillus brevis IOEB 9809, cell lysis and DNA amplification are performed in one single step, without preliminary nucleic acid extraction or purification treatments. The assay is performed in about 30 min, requiring 150 nL of starting sample and it enables the detection of down to 15 bacterial cells. With respect to traditional culture techniques, the speed, the simplicity and the cheapness of this procedure allow an effective monitoring of microbial cells during food-making and processing. 相似文献
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Tsuyoshi Nomura Masahiro Ikeda Seiji Ishiyama Kazuhide Mita Toshiki Tamura Takahiro Okada Kazuhito Fujiyama Akihiro Usami 《Journal of Bioscience and Bioengineering》2010,110(4):386-391
In insects, β-N-acetylglucosaminidase (GlcNAcase) participates in critical physiological processes such as fertilization, metamorphosis, and glycoconjugate degradation. Insects produce glycoproteins carrying paucimannosidic-type N-glycans, the terminal GlcNAc residue of which is cleaved by a GlcNAc-linkage specific GlcNAcase, also known as the fused lobes (FDL) protein. To obtain information on the structure of GlcNAcases and insight into their contribution to physiological processes, we cloned Bombyx mori FDL (BmFDL) from silkworm larvae. The full-length cDNA (1.9 kb) encoded a protein of 633 amino acids with 42% amino acid sequence identity to Drosophila melanogaster FDL (DmFDL). Recombinant BmFDL cleaved only β-1,2-linked GlcNAc residues from the α-1,3 branch of biantennary N-glycan. This substrate specificity was similar to that of DmFDL. Microsomal FDL activity was inhibited by anti-BmFDL antibodies. Taken together, our results suggest that BmFDL is a N-glycan-processing GlcNAcase in B. mori. 相似文献
6.
Bhowmik Showmik Sarkar Ram Nasipuri Mita Doermann David 《International Journal on Document Analysis and Recognition》2018,21(1-2):1-20
International Journal on Document Analysis and Recognition (IJDAR) - Separation of text and non-text is an essential processing step for any document analysis system. Therefore, it is important to... 相似文献
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Numerical assessment of the seismic response of an earth embankment on liquefiable soils 总被引:2,自引:0,他引:2
Yu Huang Atsushi Yashima Kazuhide Sawada Feng Zhang 《Bulletin of Engineering Geology and the Environment》2008,67(1):31-39
This study presents a numerical assessment of the seismic behaviour of an earth embankment founded on liquefiable foundation
soils during earthquake loading. Analysis was carried out using an effective stress-based, fully coupled, finite element method.
The behaviour of the sandy soil is described by means of a cyclic elastoplastic constitutive model which was developed within
the framework of the Armstrong–Frederick type non-linear kinematic hardening concept. The numerical method and the analysis
procedure are briefly outlined and as an example, the seismic response of an earth embankment on a saturated sand foundation
is assessed. Based on the numerical results, the distinctive patterns of seismic response of the embankment are discussed.
Special emphasis is given to the computed results of excess pore water pressures, co-seismic and post-seismic deformations,
and accelerations during the seismic excitation. It has been found that the numerical model can capture fundamental liquefaction
aspects of the embankment foundation system and produce preliminary results for its seismic assessment.
相似文献