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61.
An analytic model based on ANSYS/LS-DYNA has been developed on the cold rolling process for Q235 steel rebar with 12 mm in diameter. The elastic-plastic finite element method (FEM) and the cold deformation resistance model of Q235 steel were adopted in this model. Deformation uniformity of the final product has been analyzed using this model. The results indicate that the uniformity of the final product is obtained only as the centerline of the bending rolls is vertical to the centerline of the driven roll and parallel to the centerline of the drive roll in the whole rolling process. Besides, the number of the bending rolls must even realize the continuous bending and reverse bending process. Also, the number of the bending rolls must match the deformation degree of the workpiece in the cold rolling process. The validity of this finite element model was verified by the size and distribution of grains from the billet to the rebar in a practical cold rolling process. 相似文献
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QUAN WANG YING‐CHUN LIU JIAN LI WEI JIANG YONG‐JUN CHEN NING‐NING SONG 《Journal of food quality》2012,35(1):76-82
ABSTRACT
An indirect competitive enzyme‐linked immunosorbent assay with chemiluminescent (CL‐ELISA) detection for okadaic acid (OA) in mussel muscle was developed. A hybridoma cell line secreting monoclonal antibody against OA was established after immunization of BALB/c mice with artificially synthesized OA‐bovine serum albumin as antigen. Luminol solution was used as the substrate for horseradish peroxidase. The detection limit was 0.175 ng/g. The range of average fortified recovery was 91.8–102.6% when OA was spiked in mussel muscle at levels of 50, 160 and 800 µg/kg. The standard curve for OA showed good linearity over the concentration range of 0.08125–20 ng/mL. The cross‐reactivity with dinophysistoxin1 and saxitoxin was 51.82 and 0%, respectively. In a residue study, the results obtained by CL‐ELISA correlated well within those obtained using the commercial ABRAXIS kit (ABRAXIS, Warminster, PA). The developed method is therefore suitable for detecting the residues of OA in shellfish.PRACTICAL APPLICATIONS
Diarrhetic shellfish poisoning is a gastrointestinal syndrome that occurs in humans following the consumption of bivalve mollusks contaminated with OA with the main symptoms of diarrhea, nausea, vomiting and abdominal pain. OA widely exists at marine products and it is very important to develop a technique to monitor this toxin. In this study, a sensitive competitive indirect enzyme‐linked immunosorbent assay with chemiluminescence for determination of OA in mussel soft tissues was investigated. Chemiluminescent ELISA (CL‐ELISA) is a good alternative method for screening samples. This technique has the potential to improve the sensitivity of the immunoassays by at least two to three orders of magnitude compared with conventional colorimetric detection. 相似文献64.
要摆脱目前县域工业企业发展的窘境,必须建立企业智库,帮助在迷茫中前行的企业家崛起,以实现县域工业经济的新跨越. 相似文献
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通过电子阳极与镁阳极棒对储水式水箱内胆阴极保护理论分析与实验测试对比研究,从多方位包括水质PH值、不同水温条件、不同电导率等状态分析,对比电子阳极与镁棒阴极保护在热水器水箱上实施阴极保护的差异,分析总结出采用电子阳极技术对储水式水箱内胆腐蚀保护效果与镁棒效果一致,并且电子阳极适应范围更广,不消耗有色金属,更节能环保;不需要更换维护,使用寿命更长,经济性更高。 相似文献
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