结核分枝杆菌RD1区重组11 kD蛋白的克隆表达及效力

目的在大肠杆菌中表达结核分枝杆菌RD1区11kD蛋白,并检测表达产物的效力。方法构建11kD蛋白编码基因原核表达载体,转化大肠杆菌BL21(DE3)IPTG诱导表达,离子层析柱分离纯化重组蛋白。以结核分枝杆菌致敏豚鼠进行皮肤变态反应(DTH)测定。结果结核分枝杆菌11kD蛋白在大肠杆菌中以可溶形式表达,表达蛋白占总菌体蛋白的33%以上,纯化后纯度达95%以上,并可诱导结核分枝杆菌致敏豚鼠产生迟发型超敏反应,5μg/ml11kD蛋白的效力与50IU/ml TB-PPD相当。结论重组11kD蛋白已在大肠杆菌中成功表达,并有望成为结核病皮试鉴别诊断用新试剂。     

Male european corn borer,Ostrinia nubilalis (Hübner), antennal responses to analogs of its sex pheromone

Experiments were conducted to (1) determine whether the electroantennogram (EAG) can detect differences among the responses of antennae from males derived from the three strains ofOstrinia nubilalis (Hübner), and (2) characterize the EAG responses of each strain to isomeric forms of the natural pheromone, (E)- and (Z)-11-tetradecen-1-ol acetate (TDA), and analogs possessing differences in the terminal alkyl group, cyclopropyl (CPA), ortert-butyl (TBA).EAG responses differed among the strains in two ways: (1) Antennae fromZZ males always produced an EAG to (Z)-TDA with an extended duration of response. This signature EAG response was found to be unique to the antennal response ofZZ males to (Z)-TDA, thus providing a relatively easy method of distinguishing liveZZ males fromEE orZE males. Correlated with this longer EAG response was a longer disadaptation time, i.e., the EAG response ofZZ antennae disadapted more slowly (ca. 10 min) than the response ofEE antennae. (2) Strain differences in the relative EAG amplitudes to isomers and analogs were observed at the stimulus amounts eliciting the peak EAG amplitude as follows: TDA CPA > TBA forZZ males and both isomers; TDA > CPA TBA and CPA TDA > TBA forEE males and theE andZ isomers, respectively; CPA > TBA TDA forZE males and both isomers. Dose—response relationships were seen for all compounds if amplitude (peak height) of the EAG was used as a measure of response. However, if width of the EAG at half the peak height (peak width) was used, then only theZZ antennal response to (Z)-TDA resulted in a meaningful dose-response relationship. For all strains, the EAG amplitudes elicited by theZ isomers of any of the tested compounds were greater than those elicited by the correspondingE isomers. Therefore, correlations between the relative EAG and upwind flight responses were observed in theZZ (r = 0.86) andZE (r = 0.80) strains but were not correlated in theEE strain (r = 0.18). Temporal studies showed that adaptation, not postexcision deterioration, was responsible for the observed decreases in the EAG amplitude after repetitive stimulation or after stimulation with amounts in a descending order. Disa-daptation required at least 20 min for a moderate dose (10 g for 1 sec). Developmental studies showed that antennae from 2-day-old adults had the greatest EAG response.     

Sr_2Al_6O_(11):Eu~(2+),Dy~(3+)蓝色发光材料制备

用高温固相法合成Sr2Al6O11:Eu2+,Dy3+蓝色发光材料,研究了合成温度、保温时间、B2O3加入量对该材料物相组成和发光性能的影响。结果表明,只有在掺入一定量的B2O3(5%~15%)的条件下,通过控制温度(1000℃~1150℃)和保温时间(1h~2h)能够合成Sr2Al6O11:Eu2+,Dy3+蓝色发光材料。     

基于临近空间的预警系统建设研究

针对来自空、天两个层面的强大威胁,仅依靠现有的预警手段和系统很难有效应对.通过分析空天一体信息作战的威胁与现状,阐述了临近空间特性并分析了在临近空间部署预警系统的优势：对上可以弥补天基系统,对下可以填补空基系统和陆基系统的缝隙,且可控性强.最后就作为临近空间防空预警系统、区域战场侦察监视系统、指挥通信系统以及集成临近空间电子对抗系统等提出了发展临近空间预警系统建设的建议.     

SAPO-11型分子筛水热合成改进工艺研究

使用廉价的二乙胺为模板剂,以合适的硅源、磷源、铝源,采用先陈化后晶化的合成方式,合成了SAPO-11分子筛,并研究了不同因素对SAPO-11分子筛合成的影响．结果表明：二乙胺为SAPO-11分子筛合成的合适模板剂,不同的加料方式对分子筛的合成有影响,最佳的陈化时间和晶化时间分别为24h,36h．     

Hot-compression behavior of Al-1Mn-1Mg alloy

The hot-compression of Al-1Mn-1Mg (mass fraction, %) alloy sample was carried out on a Gleeble-1500 thermo-simulator at deformation temperatures from 320 to 400 ℃ and strain rates from 0.1 to 10 s-1 by total strain of 1.4. Microstructure and texture evolution of the hot-compressed alloy were investigated by optical microscopy and X-ray diffraction analysis, respectively. The results show that the relationship among flow stress σ, deformation temperature T and strain rate ε can be expressed in the form of βσ...     

Influence of surface finish and annealing treatment on oxidation behavior of Ti-48Al-8Cr-2Ag alloy

The effect of surface finish and annealing treatment on the oxidation behavior of Ti-48Al-8Cr-2Ag (molar fraction, %) alloy was investigated at 900 and 1 000 °C, respectively in air. Thermal gravimetric analysis (TGA) was conducted for the characterization of oxidation kinetics. The microstructures of oxide scales were studied by scanning electron microscopy (SEM) and transmission election microscopy (TEM) techniques. Unfavorable effect of the annealing treatment on the oxidation behavior of the coating was also investigated. The results indicate that the oxidation behavior of the alloy is influenced by surface finish and annealing treatment. The oxidation rate of ground sample is lower than that of the polished alloy at 1 000 °C in air. The former forms a scale of merely Al₂O₃, and the latter forms a scale of the mixture of Al₂O₃ and TiO₂. Annealing can improve the formation of TiO₂. Foundation item: Project(2007430028) supported by the Science and Technique Foundation of Henan Educational Committee, China     

凝析气藏注气过程中露点压力变化特征研究

在凝析气藏循环注气和注气吞吐解除近井带反凝析堵塞的注气过程中,凝析气流体的露点压力变化较大,而在目前的凝析气藏开发过程中,对凝析气露点压力变化考虑较少.作者利用Nemeth.L.K和Kennedy.H.T.的露点压力计算经验公式,研究了向凝析气中注入N2、甲烷、CO2和丙烷等介质的过程中露点压力变化规律,并分析了衰竭式开发和注气过程中凝析气井近井带露点压力变化特征.结果表明,注甲烷使露点压力有较小幅度的减小;注入CO2和丙烷均可使凝析气露点压力有较大幅度降低;而注入N2将使露点压力升高.在凝析气藏衰竭式开发过程中,当近井带压力下降至露点压力以下时,距井筒越近,凝析气中重质组分含量越低,露点压力也越低.在注气吞吐的焖井阶段,反蒸发作用会引起凝析气中重质组分增加,露点压力将升高.     

作物病害胁迫检测的研究进展

综述了基于遥感技术、图像处理技术和农业专家系统的作物病害胁迫检测方法,比较这三种方法研究进展及其优缺点,介绍了近场声全息技术研究现状,提出了基于近场声全息理论的作物病害胁迫检测的研究方法,为作物病害综合精准防治开辟了一条新的途径．     

临近空间通信网RA/DAMA协议时延性能分析

近年来,临近空间通信网络开始成为新的研究热点,但对临近空间通信网多址接入协议的研究较少,且已发表的论文仅关注固定终端。为解决移动终端对临近空间物理链路资源有效共享的问题,提出一种随机预约/按需分配多址接入协议（RA/DAMA）,运用排队理论对其平均时延公式进行了推导,并利用OPNET软件进行了仿真分析。仿真结果表明,随着网络负载的增加,网络的平均时延越来越大;相同的网络负载情况下,时帧周期越长,网络的平均时延越大,且时延性能越不稳定。