末端含1,3,4-噻二唑聚苯乙烯的ATRP合成及其荧光性能研究

合成了化合物2-苯基-5-(4-溴甲苯基)-1,3,4-噻二唑(PBrMPTDA),并以其作为引发剂、PMDETA/CuBr为催化剂、环己酮为溶剂,实现了苯乙烯的原子转移自由基聚合(ATRP).一级动力学曲线证明该聚合反应符合"活性"/可控自由基聚合,同时讨论了配体用量、温度和引发剂浓度对聚合速率的影响,发现在100℃下n(St)/n(PBrMPTDA)/n(CuBr)/n(PMDETA)=200/1/1/1能较有效控制苯乙烯的原子转移自由基聚合,所得聚合物相对分子质量分布(mw/mn)较窄(1.22～1.50).对聚苯乙烯的荧光光谱研究发现,端基功能化的聚苯乙烯在390nm处有很强的荧光.     

Syntheses and applications of Eu（Ⅲ） complexes of 2-thienyltrifluoroacetonate, terephthalic acid and phenanthroline as light conversion agents

A series of europium(Ⅲ) complexes of 2-thienyltrifluoroacetonate (HTTA), terephthalic acid (TPA) and phenanthroline (Phen) were synthesized. The new complexes Eu(TPA)(TTA)Phen and Eu2(TPA)(TTA)4Phen2 were characterized by elemental analysis, IR spectrum, scanning electron microscope and thermal stability analysis. The results show that the thermal stability of the Eu( Ⅲ ) complexes increases in the following order: the mononuclear complex Eu(TTA)3Phen, the binuclear complex Eu2(TPA)(TTA)4Phen2, the chain polynuclear complex Eu(TPA)(TTA)Phen. And the formation of the binuclear/polynuclear structure of the new complexes appears to be responsible for the enhancement of their thermal and optical stability. In addition, The fluorescence excitation spectra of these new complexes show more broad excitation bands than that of the complex Eu(TTA)3Phen corresponding to their formation. The enhancement of Eu3 fluorescence in the new complexes can be observed by the addition of Gd3 . The bright red luminescent plastics can be obtained when the complex EuGd(TPA)(TTA)4Phen2 is added above 0.5% (mass fraction).     

大肠杆菌无细胞蛋白质合成系统的初步研究

陈海琴，徐志南，汪诚，廖玉华，岑沛霖在近几年来被广泛研究和应用的大肠杆菌无细胞蛋白质合成系统中，大肠杆菌抽提物的制备、底物浓度的配比以及能量再生体系的选择是最关键的几个因素.以绿色荧光蛋白(GFP)为报导基因，以缺失核酸酶I基因的大肠杆菌A19为实验菌株，通过对大肠杆菌无细胞抽提物制备过程中菌体收集时间、细胞破碎压力、后处理条件等参数的摸索和确定、无细胞蛋白质合成系统中底物浓度配比的正交实验优化以及系统中能量再生体系的比较和选择，制备得到了GFP表达量为154 μg/mL的大肠杆菌无细胞蛋白质合成系统，与优化前相比产量提高了29倍.     

Fluorescent silicon nanoparticle-based gene carriers featuring strong photostability and feeble cytotoxicity

Safe fluorescent gene-transfection vectors are in great demand for basic biological applications and for gene-therapy research. Here, we introduce a new type of luminescent silicon nanoparticle (SiNP)-based gene carrier suitable for determining the intracellular fate of the gene vehicle in a long-term and real-time manner. The presented SiNP-based nanocarriers simultaneously feature strong and stable fluorescence, high DNA-loading capacity and gene-transfection efficiency, as well as favorable biocompatibility. Taking advantage of these unique benefits, we were able to readily observe the behavior of the gene carriers in live cells (e.g. cellular uptake, intracellular trafficking, and endosomal escape) in a long-term and real-time manner. The results demonstrate the potential usability of these fluorescent SiNP-based gene vectors as powerful tools in the field of gene therapy, and provide invaluable information for understanding the intracellular behavior of gene carriers.

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Dicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae

Fluorescent proteins are convenient tools for measuring protein expression levels in the budding yeast Saccharomyces cerevisiae. Co‐expression of proteins from distinct vectors has been seen by fluorescence microscopy; however, the expression of two fluorescent proteins on the same vector would allow for monitoring of linked events. We engineered constructs to allow dicistronic expression of red and green fluorescent proteins and found that expression levels of the proteins correlated with their order in the DNA sequence, with the protein encoded by the 5′‐gene more highly expressed. To increase expression levels of the second gene, we tested four regulatory elements inserted between the two genes: the IRES sequences for the YAP1 and p150 genes, and the promoters for the TEF1 gene from both S. cerevisiae and Ashbya gossypii. We generated constructs encoding the truncated ADH1 promoter driving expression of the red protein, yeast‐enhanced Cherry, followed by a regulatory element driving expression of the green protein, yeast‐enhanced GFP. Three of the four regulatory elements successfully enhanced expression of the second gene in our dicistronic construct. We have developed a method to express two genes simultaneously from one vector. Both genes are codon‐optimized to produce high protein levels in yeast, and the protein products can be visualized by microscopy or flow cytometry. With this method of regulation, the two genes can be driven in a dicistronic manner, with one protein marking cells harbouring the vector and the other protein free to mark any event of interest. Copyright © 2009 John Wiley & Sons, Ltd.     

Progress on Study of Luminescence of Rare Earth Organic Chelates

Ｓｉｎｃｅｒａｒｅｅａｒｔｈｃｈｅｌａｔｅｓｗｅｒｅｆｏｕｎｄｔｏｅｍｉｔｌａｓｅｒｉｎｔｈｅｂｅｇｉｎｎｉｎｇｏｆ 1 96 0ｓ ,ｔｈｅｆｌｕｏ ｒｅｓｃｅｎｃｅｐｒｏｐｅｒｔｉｅｓ ,ｅｆｆｉｃｉｅｎｃｙａｎｄｌｉｆｅｔｉｍｅｆｏｒｒａｒｅｅａｒｔｈｏｒｇａｎｉｃｃｈｅｌａｔｅｓｗｅｒｅｅｘｔｅｎｓｉｖｅｌｙｓｔｕｄｉｅｄ .Ｔｈｅｃｈｅｌａｔｅｓｗｅｒｅｐｒｏｖｅｄｎｏｔｔｏｂｅｓｕｉｔａｂｌｅｆｏｒｌａｓｅｒｍａｔｅｒｉａｌｓｉｎｔｈｅｅｎｄｏｆ1 970ｓ .Ｓｏｉｎｉｓｕｇｇｅｓｔｅｄｔｏｕｓｅｒａ…     

A 30,000-cd/m2, 50-lm/W,and 92%-luminance-uniformity flat discharge fluorescent lamp for LCD backlights

Abstract— A 5.2-in.-diagonal simple-structured argon-mercury cold-cathode flat discharge fluorescent lamp has been developed for LCD backlighting. A pair of insulated electrodes is provided at the top and bottom ends of the inner surface of the front glass plate. Phosphor is deposited on both the front and rear glass plates. A luminance of 30,000 cd/m² and a luminous efficacy of 50 lm/W were obtained with a luminance uniformity of 92% without the use of a diffuser sheet. A mechanism for obtaining the high luminous efficacy is described. Luminance can be dimmed down to 4% of the peak value by extending the pulse interval and/or by reducing the pulse amplitude.     

Luminescent Conjugated Oligothiophenes for Sensitive Fluorescent Assignment of Protein Inclusion Bodies

Small hydrophobic ligands identifying intracellular protein deposits are of great interest, as protein inclusion bodies are the pathological hallmark of several degenerative diseases. Here we report that fluorescent amyloid ligands, termed luminescent conjugated oligothiophenes (LCOs), rapidly and with high sensitivity detect protein inclusion bodies in skeletal muscle tissue from patients with sporadic inclusion body myositis (s‐IBM). LCOs having a conjugated backbone of at least five thiophene units emitted strong fluorescence upon binding, and showed co‐localization with proteins reported to accumulate in s‐IBM protein inclusion bodies. Compared with conventional amyloid ligands, LCOs identified a larger fraction of immunopositive inclusion bodies. When the conjugated thiophene backbone was extended with terminal carboxyl groups, the LCO revealed striking spectral differences between distinct protein inclusion bodies. We conclude that 1) LCOs are sensitive, rapid and powerful tools for identifying protein inclusion bodies and 2) LCOs identify a wider range of protein inclusion bodies than conventional amyloid ligands.