The synthesis and characterization of surface-active compounds based on various steroid derivatives and glucose are presented.
The hydrophobic and hydrophilic parts of the compounds were linked via glucosidic bonds. All compounds were found to have
very low water solubility and only limited solubility in various organic solvents. The compounds were investigated according
to their ability to interact with an anionic surfactant, sodium dodecylsulfate (SDS), in order to induce a decrease in the
critical micelle concentration (CMC) of the surfactants. This synergistic effect was pronounced for cholestanol-6-on-β-D-glucopyranoside and for cholestan-3,6-diol-β-D-glucopyranoside. These two compounds lowered the CMC from 8 to 6 and 0.6 mM, respectively, for water solutions of SDS/glucoside
with a molar ratio of 92∶8. Furthermore, the ability of the compounds to stabilize lipid membranes, in liposomes, at varying
concentrations of Ca2+, was studied. The compounds were, as expected, found to induced stabilization similar to that of cholesterol. 相似文献
The alterations caused by different surfactants in the permeability of liposomes formed by a lipid mixture that models the
stratum corneum (SC) composition (40% ceramides, 25% cholesterol, 25% palmitic acid, and 10% cholesteryl sulfate) were investigated.
The surfactant/lipid molar ratios (Re) and the bilayer/aqueous phase surfactant partition coefficients (K) were determined at two sublytic levels. The selected surfactant were sodium dodecyl sulfate (SDS); sodium dodecyl ether
sulfate (SDES) to assess the influence of the ethylene oxide groups on the anionic surfactant’s behavior; Triton X-100 (OP-10EO)
and dodecyl betaine (D-Bet) as representatives of nonionic and amphoteric surfactants. Permeability alterations were determined
by monitoring the increase in the fluorescence intensity of liposomes due to the 5(6) carboxyfluorescein (CF) released from
the interior of vesicles. The SC liposomes/surfactant sublytic interactions were mainly ruled by the action of surfactant
monomers. OP-10EO showed the highest ability to alter the permeability of bilayers and the highest affinity with these structures,
whereas D-Bet showed the lowest tendencies. Although SDS and SDES exhibited similar activity at 50% CF release (similar Re values), SDES appeared to be more active at 100% CF release, its affinity with bilayers being also increased. The different
ability exhibited by SDS, SDES, and D-Bet (same alkyl chainlength) to alter the permeability of SC liposomes emphasizes the
role played by the polar part of these surfactants in this interaction. Different trends in the evolution of Re and K were observed when comparing the results with those reported for phosphatidylcholine (PC) liposomes. Thus, whereas SC liposomes
appeared to be more resistant to the action of surfactants, the surfactant affinity with SC bilayers was always greater than
that reported for PC bilayers. 相似文献
Context: Long-circulation (PEGLip), pH-sensitive (PEOzLip), and active targeted liposomes (PEG-TATLip)-loading doxorubicin (DOX) and harmine (HM) were prepared. Their physicochemical properties and antitumor effect were investigated.
Objectives: The aims of the present study were to evaluate synergistic antitumor efficacy.
Materials and methods: Liposomes were prepared by using thin-film dispersion, active drug-loading and target post-insertion method. Subsequently physiochemical properties including particle size distribution, zeta potential, entrapment efficiency (EE), drug-loading content and in-vitro release were determined. Besides, the in vitro cytotoxicity of free drugs and drug-loaded liposomes was explored by using a Sulforhodamine-B Staining assay and the combination index values (CI Value) were calculated. Finally, the cellular uptake experiments by MCF-7cells were carried out via flow cytometry.
Results and discussion: All liposomes enhanced the antitumor effect significantly compared to free drugs. Among liposomes, PEG-TATLip enhanced the antitumor effect significantly compared to others. DOX and HM had moderate synergism with CI Value 0.85 for free drugs, 0.81 for PEGLip, 0.72 for PEOzLip, and 0.84 for PEG-TATLip respectively when the weight ratio of two drugs was 1:2. Moreover, the similarity between DOX and HM such as physicochemical properties, in vitro release modes and in vitro uptake kinetics characteristics when they were in the same formulations proved it possible for them to be delivered together.
Conclusion: Active targeting liposomes were the most effective delivery system as compared with pH-sensitive and long circulation liposomes. Additionally, DOX and HM could be co-delivered in liposomes and they could play moderate synergism effect in antitumor efficacy. 相似文献