气相色谱法检测消毒产品中的正丙醇与乙醇

使用HP-Plot Q色谱柱与FID检测器检测消毒剂中复配的正丙醇与乙醇含量,最大方法相对偏差(RSD)为2.14%,最小方法回收率为100.0%,乙醇与正丙醇的检出浓度为0.025%,是一种快速准确的一针检测法。     

MS Binding Assays for Glycine Transporter 2 (GlyT2) Employing Org25543 as Reporter Ligand

This study describes the first binding assay for glycine transporter 2 (GlyT2) following the concept of MS Binding Assays. The selective GlyT2 inhibitor Org25543 was employed as a reporter ligand and it was quantified with a highly sensitive and rapid LC-ESI-MS/MS method. Binding of Org25543 at GlyT2 was characterized in kinetic and saturation experiments with an off-rate of 7.07×10⁻³ s⁻¹, an on-rate of 1.01×10⁶ M⁻¹ s⁻¹, and an equilibrium dissociation constant of 7.45 nM. Furthermore, the inhibitory constants of 19 GlyT ligands were determined in competition experiments. The validity of the GlyT2 affinities determined with the binding assay was examined by a comparison with published inhibitory potencies from various functional assays. With the capability for affinity determination towards GlyT2 the developed MS Binding Assays provide the first tool for affinity profiling of potential ligands and it represents a valuable new alternative to functional assays addressing GlyT2.     

Continuous size fractionation of magnetic nanoparticles by using simulated moving bed chromatography

The size fractionation of magnetic nanoparticles is a technical problem, which until today can only be solved with great effort. Nevertheless, there is an important demand for nanoparticles with sharp size distributions, for example for medical technology or sensor technology. Using magnetic chromatography, we show a promising method for fractionation of magnetic nanoparticles with respect to their size and/or magnetic properties. This was achieved by passing magnetic nanoparticles through a packed bed of fine steel spheres with which they interact magnetically because single domain ferro-/ferrimagnetic nanoparticles show a spontaneous magnetization. Since the strength of this interaction is related to particle size, the principle is suitable for size fractionation. This concept was transferred into a continuous process in this work using a so-called simulated moving bed chromatography. Applying a suspension of magnetic nanoparticles within a size range from 20 to 120 nm, the process showed a separation sharpness of up to 0.52 with recovery rates of 100%. The continuous feed stream of magnetic nanoparticles could be fractionated with a space-time-yield of up to 5 mg/(L∙min). Due to the easy scalability of continuous chromatography, the process is a promising approach for the efficient fractionation of industrially relevant amounts of magnetic nanoparticles.     

A new partitioning process for high-level liquid waste by extraction chromatography using silica-substrate chelating agent impregnated adsorbents

To separate the long-lived minor actinides (MA(III) = Am(III), Cm(III)) and some specific fission products (FP) such as Pd(II), Mo(VI), Cs(I) and Sr(II) from high-level liquid waste (HLLW), we have been studying a new partitioning process by extraction chromatography using several novel silica-based extraction resins. In this work, we examined the separation behavior of the elements contained in a simulated MA-effluent by the CMPO/SiO₂P packed column. In addition, as an attempt to further isolate Am(III) and Cm(III) from the heavy RE(III) such as Eu(III), Gd(III) and Dy(III) contained in the MA-effluent, we investigated the possibility of separation by using a silica-based cationic exchange resin. Furthermore, to isolate Sr(II) from the HLLW, adsorption and separation performances of Sr(II) and some other FP elements were studied by using a novel silica-based crown ether extraction resin, DtBuCH18C6/SiO₂P. The experimental results demonstrated that the elements in the simulated MA-effluent can be successfully separated to (1) Pd, (2) MA-hRE and (3) Zr–Mo, by CMPO/SiO₂P packed column using water and a dilute DTPA solution as eluents. Am(III) and Cm(III) are expected to be effectively separated from light RE(III) and Y(III) by the SiSCR cationic resin. However, more effective separation between Am(II), Cm(III) and heavy RE(III) such as Eu(III), Gd(III) and Dy(III) needs further approach. DtBuCH18C6/SiO₂P showed a highly selective adsorption for Sr(III) so that the Sr(II) could be completely separated from other FPs except a portion of Ba(II).     

SMB色谱分离过程计算机仿真

针对模拟移动床(SMB)综合速率模型，采用有限元法和正交配点法分别对柱向和颗粒径向模型进行离散化，利用MatLab ODE求解器对SMB过程进行数值求解。通过仿真，验证了该方法的可行性。     

大豆中异黄酮类化学成分的研究

用w(C2H5OH)=70%的工业乙醇从脱脂大豆饼中提取大豆异黄酮,并依次用乙酸乙酯、正丁醇萃取,乙酸乙酯萃取部分经硅胶柱和SephadexLH-20柱层析,用V(CHCl3)∶V(CH3OH)=(12～4)∶1梯度洗脱。分离得到4种化合物,通过理化常数和光谱分析鉴定其化学结构,分别为大豆甙元(daidzein)、染料木素(genistein)、大豆甙(daidzin)、染料木甙(genistin)。     

木瓜凝乳蛋白酶的分离纯化及性质研究

目的　建立简单有效的木瓜凝乳蛋白酶分离纯化方法 ,并研究其酶学性质。方法　采用离子交换层析直接将木瓜凝乳蛋白酶分离纯化 ,通过温度、葡萄糖、盐酸胍及其他因素研究酶学性质。结果　木瓜凝乳蛋白酶有较高的热稳定性 ,盐酸胍对其有抑制作用 ,葡萄糖对其有激活作用。结论　离子交换层析是简单有效的分离纯化木瓜凝乳蛋白酶的方法 ,木瓜凝乳蛋白酶值得进一步开发利用。     

预分离色谱法直接测定微量组份无限稀释活度系数

预分离色谱法可直接测定混合物中微量组份的无限稀释活度系数(γ~∞),而不必把此组份从混合物中分离出来。主要由四步组成:被测组份的预浓缩,被测组份的定性,选择预分离柱和γ~∞的测定。预浓缩样品经过预分离柱分出被测组份,然后进入测量柱测定γ~∞。此方法简捷、可靠与常规以纯溶质进样的色谱测y~∞的方法具有相同的测定精度,可广泛用于缺少纯样的场合。用此法测定了戊烯-1-炔-4、丁炔-2在若干酸醇中的γ~∞,分别求得了修正和未修正的UNlFAC参数,对kikic修正模型其值为ac=c,CooH=694.9(K)acooH,c=c=-99.08(K),ac=c,oH=812.99(K),aoH,c=c=143.36(K)     

采用气相色谱法检测精脱萘循环油中的萘含量

2003年济钢焦化厂引进焦炉煤气精脱萘工艺,利用柴油循环脱萘。鉴于精脱萘工艺柴油成分复杂,混合物性质极为相近的特点,开发了采用气相色谱法测定循环柴油中萘含量的分析方法,并确定了其检测条件。该法操作简便、快速,数据准确,其检测结果为精脱萘工艺生产提供了及时、准确的指导数据。