ICP-MS法测定野生及种植北五味子中的微量元素

利用ICP-MS对长白山区野生和种植五味子中的微量元素进行了测定和分析.结果表明：在选定的实验条件下,方法的检出限为0.002～0.092ng/mL,相对标准偏差RSD在1.87%～4.96%之间,标准加入回收率在94.0%～104.5%之间;野生五味子中的微量元素的总量明显高于种植的,并且有害元素铅、砷和镉的含量均低于种植五味子.     

Protective effect of HV-P411, an herbal mixture,on carbon tetrachloride-induced liver fibrosis

This study was performed to examine the anti-fibrotic activity of HV-P411, an herbal mixture of seeds of Vitis vinifera, Schisandra chinensis and Taraxacum officinale extract, against carbon tetrachloride (CCl₄)-induced liver fibrosis. Hepatic fibrosis was induced by intraperitoneal injection of CCl₄ (0.5 ml/kg, twice weekly) for 8 weeks. Rats were treated orally with HV-P411 at 50, 100, 200, and 400 mg/kg once a day. After chronic exposure to CCl₄, the levels of hydroxyproline were markedly increased; these were significantly reduced by HV-P411 at all dose levels. The level of serum aminotransferases and lipid peroxidation were increased after the CCl₄ treatment, while reduced glutathione was decreased. These changes were attenuated by HV-P411. In addition, HV-P411 attenuated CCl₄-induced raised serum concentration of transforming growth factor-β1, and the levels of matrix metalloprotease-2 and tissue inhibitor of metalloprotease-1 mRNAs. Our results suggest that HV-P411 may prevent liver fibrosis by modulating fibrogenesis and fibrolysis.     

中华猕猴桃种子提取不饱和脂肪酸的研究

用中华猕猴桃种子提取不饱和脂肪酸 ,宜用石油醚作萃取剂 ,经过萃取、脱蜡、浓缩、精制后 ,其得率约为种子质量的 2 8% ,经分析 ,其亚麻酸与亚油酸含量占总脂肪酸含量的 89.4 %。     

五味子酿酒工艺及配方优化研究

以野生北五味子果实为原料，对三种原酒制备工艺进行了对比研究，结果以新鲜果实发酵质量最好。通过正交设计，时成品酒配方进行了筛选并优化。     

RP-HPLC测定南、北五味子中三种木脂素类成分的含量

采用反相高效液相色谱(RP-HPLC)法检测五味子中三种木脂素类成分的含量。色谱柱为Agilent C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(75∶25),流速1.0 m L/min,检测波长250 nm,柱温30℃,进样量10μL。结果表明,3种成分的进样量分别在4~800、4~400、4~400μg/m L范围与各自峰面积积分值呈良好的线性关系(r分别为0.999 3,0.999 8,0.999 9),平均回收率分别为98.44%,98.86%和97.84%,RSD分别为1.24%,1.87%和1.18%(n均为5)。北五味子中五味子醇甲、五味子甲素和五味子乙素含量分别为7.196,1.344,3.476 mg/g,南五味子中不含五味子醇甲,五味子甲素、五味子乙素的含量依次为59.065,3.213 mg/g。该方法快速、准确、重现性好,可用于南、北五味子中木脂素类成分的分析,评价不同来源五味子的质量。     

北五味子的超临界萃取及产物的HPLC-APCI-MS/MS分析

优化超临界流体萃取北五味子中木脂素类成分的提取工艺,对萃取物中所含有效成分的归属进行分析鉴定.以五味子总木脂素为评价指标,采用正交实验法和不同影响因素系统考察法对CO2 -SFE萃取北五味子中木脂素类成分提取工艺进行优化,利用液相色谱-大气压化学电离质谱联用法(HPLC-APCI-MS/MS)分析鉴定产物结构.确定了最...     

维生素C对中国对虾的营养和免疫作用及其最适剂型

论述了维生素Ｃ对中国对虾的营养作用，对对虾蜕壳的影响，以及对虾对维生素Ｃ的缺乏症；ＬＡＰＰ对中国对虾缺氧耐受力及免疫抵抗力的影响；各类维生素Ｃ产品在不同条件下的稳定性；不同剂型维生素Ｃ对中国对虾生长的影响。     

Angiotensin I-converting enzyme inhibitory activity of Acetes chinensis peptic hydrolysate and its antihypertensive effect in spontaneously hypertensive rats

Peptic hydrolysate with angiotensin I-converting enzyme (ACE) inhibitory activity was prepared from Acetes chinensis . The 3 kDa ultrafiltration filtrates (UF-IV) of the desalted hydrolysate exerted a potent ACE inhibitory activity with IC50 being 0.67 mg mL⁻¹. The fractions with molecular weight located between 611 and 240 Da achieved a total percentage of 63.6% in the UF-IV, with a mean peptide length of about 5.6–2.1. Gastrointestinal digestions were simulated on the hydrolysate and UF-IV and addressed their effect on the ACE inhibitory activity. The ACE inhibitory activity of the UF-IV could survive or be maintained in active form by simulated gastrointestinal digestion. Furthermore, the UF-IV exerted potent antihypertensive effect after oral administrations were given to spontaneously hypertensive rats (SHR) at a dose of 900 mg kg⁻¹ body weight. In conclusion, the UF-IV could serve as useful antihypertensive peptides in the prevention and treatment of hypertension and other associated disorders.     

Simultaneous determination of nine lignans using pressurized liquid extraction and HPLC-DAD in the fruits of Schisandra chinensis

The pressurized liquid extraction and HPLC-DAD was developed for extraction and determination of bioactive lignans in Schisandra chinensis. The efficient PLE conditions employed methanol as extraction solvent, 125 °C of extraction temperature, 5 min of static extraction time and only one cycle. A rapid HPLC-DAD method was described for simultaneous determination of nine lignans, including schisandrol A, gomisin J, schisandrol B, tigloylgomisin H, angeloylgomisin H, schisandrin A, γ-schisandrin, gomisin N and schisandrin C. The extraction efficiency of PLE was observed to be comparable with reflux and sonication. In addition, the contents of nine lignans in S. chinensis from different regions were analysed by PLE and HPLC-DAD method.