GOLPH3 Regulates EGFR in T98G Glioblastoma Cells by Modulating Its Glycosylation and Ubiquitylation

Protein trafficking is altered when normal cells acquire a tumor phenotype. A key subcellular compartment in regulating protein trafficking is the Golgi apparatus, but its role in carcinogenesis is still not well defined. Golgi phosphoprotein 3 (GOLPH3), a peripheral membrane protein mostly localized at the trans-Golgi network, is overexpressed in several tumor types including glioblastoma multiforme (GBM), the most lethal primary brain tumor. Moreover, GOLPH3 is currently considered an oncoprotein, however its precise function in GBM is not fully understood. Here, we analyzed in T98G cells of GBM, which express high levels of epidermal growth factor receptor (EGFR), the effect of stable RNAi-mediated knockdown of GOLPH3. We found that silencing GOLPH3 caused a significant reduction in the proliferation of T98G cells and an unexpected increase in total EGFR levels, even at the cell surface, which was however less prone to ligand-induced autophosphorylation. Furthermore, silencing GOLPH3 decreased EGFR sialylation and fucosylation, which correlated with delayed ligand-induced EGFR downregulation and its accumulation at endo-lysosomal compartments. Finally, we found that EGF failed at promoting EGFR ubiquitylation when the levels of GOLPH3 were reduced. Altogether, our results show that GOLPH3 in T98G cells regulates the endocytic trafficking and activation of EGFR likely by affecting its extent of glycosylation and ubiquitylation.     

药用辅料对CYP3A活性的影响及其对仿制药质量和疗效一致性评价的指导作用

药物制剂的处方和工艺是保证药物质量和疗效的基础，药用辅料是药物制剂处方的重要组成部分。部分药用辅料可影响CYP3A的活性，继而可能影响其底物在体内的代谢和生物利用度。在中国和美国，相对生物利用度是仿制药研究的关键内容。我国正在推进仿制药质量和疗效一致性评价。因此，了解药用辅料对CYP3A的影响及其对一致性评价的指导作用具有十分重要的现实意义。     

Random poly(ε‐caprolactone‐L‐alanine) by direct melt copolymerization

A series of random polyesteramides (PEAs) with a range of molar composition from 90/10 to 50/50 were synthesized by direct melt polycondensation of ε‐caprolactone and l ‐alanine. Their structure was fully characterized by Fourier transform IR and NMR spectroscopy. The resulting copolymers are completely amorphous with the exception of PEA‐90/10 which possesses a semicrystalline structure. These PEAs present increasing glass transition temperatures at increasing l ‐alanine contents and exhibit fairly good thermal stability with 10% mass loss temperatures reaching 315 °C. © 2020 Society of Industrial Chemistry     

CVD涂层α-Al2O3织构制备技术研究

采用化学气相沉积法在硬质合金基体上沉积具有不同织构择优的α-Al2O3涂层,通过扫描电子显微镜(SEM)、X射线衍射(XRD)分别对其微观组织和机械性能进行分析。研究结果表明,通过改善过渡层的氧化气氛,氧化铝的过渡层结构为针状物,成功制备不同织构的氧化铝涂层,涂层结合力良好。     

火成岩侵入区煤层巷道支护方案数值模拟分析

本文以永定庄矿3-5^#层盘区皮带巷为研究对象,针对火成岩侵入区掘进顶板易冒落、锚索锚固效果差等工程问题,对破碎全煤巷进行了支护设计。根据火成岩侵入区巷道优化设计试验段的实际工程情况,建立了合理的数值模型,对掘进期间巷道的支护效果进行模拟分析,确定了最优的巷道支护方案。     

Common Mode Voltage Reduction Using 3D-SVPWM for 3-level CI-NPC Inverter with Hybrid Energy System

This paper elucidates Common Mode Voltage (CMV) reduction in transformerless 3-phase 3-level Coupled Inductor Neutral Point (CI-NPC) Clamped Inverter with Hybrid Energy System. The three dimensional Space Vector Pulse Width Modulation (3D-SVPWM) with Nearest State Vector (NSV) is implemented to reduce the CMV by proper selection of medium, large and small vectors in 3D cubic space region. This NSV scheme in addition to CMV reduction, reduces the capacitor voltage balancing issues and minimizes switching losses. The proposed control provides full utilization of dc link voltage with reduced harmonics. This 3-level CI-NPC inverter is energized by hybrid energy source which includes photovoltaic system and wind energy system. The results obtained for the proposed scheme through simulation and experimental setup is compared with the conventional 2D-SVPWM and 3D-SVPWM scheme. From the compared results it is evident that the proposed scheme reduces CMV to a larger extent than 2D and 3D-SVPWM control. The simulation and experimental results are verified using matlab-simulink and FPGA-Spartan-6 controller respectively.     

三维激光扫描技术在矿山主溜井测量中的应用

溜井测量具有一定的隐蔽性和不确定性,测量时有相当的危险性,如何对溜井的空间形态特征和冒落状况等进行量化评判,是所有矿山面临的难题。本文介绍了三维激光扫描技术的作业流程,给出了点云数据处理的过程和方法,并以安徽开发矿业有限公司3#主溜井测量为例进行数据采集和处理分析,建立了主溜井的三维立体模型,计算得到了主溜井需要修复的体积,为溜井的安全治理和维修设计提供了可靠的数据依据。实践结果表明了三维激光扫描技术在溜井测量中应用的可行性和优越性。     

3D and simplified pseudo-2D modeling of single cell of a high temperature solid oxide fuel cell to be used for online control strategies

In the current study, a single cell of a planar SOFC is firstly modeled in 3D using commercial SOFC module of ANSYS Fluent and the results are validated against the experimental investigations in the literature. Many researchers have used ANSYS Fluent for simulating solid oxide fuel cells. However, there is a huge gap in the literature on explaining the detailed procedure that should be followed in order to use this software effectively. A thorough step-by-step approach is presented to provide a deep insight into the software. Thereafter, a simplified quasi-2D method with infinitely shorter computational time is developed and the results are compared with the 3D model. It is found that the reduced model is capable of being utilized as an alternate method for both online diagnosis and designing active control strategies.     

温拌LDPE改性沥青混合料性能研究

选择Sasobit和Evotherm 3G两种温拌剂对温拌LDPE沥青混合料制备工艺和性能进行研究。首先,比较变温等体积法和等黏温度法两种方法,并确定两种温拌混合料的拌和及击实温度。在此基础上,分别采用车辙试验、低温弯曲试验、中点加载弯曲试验以及冻融劈裂试验评价70#、LDPE、Sasobit-LDPE以及3G-LDPE四种沥青混合料的高温性能、低温性能、疲劳性能和水稳定性。结果表明,两种温拌技术均是可行的,Sasobit和3G温拌技术可使施工温度降低20℃和15℃。与热拌LDPE沥青混合料相比,Sasobit温拌沥青混合料具有更好的高温性能和水稳定性,在高应力比条件下抗疲劳性能更好,低温性能虽变差,但是优于70#沥青混合料;3G温拌沥青混合料的高温性能,低温性能以及水稳定性则与热拌LDPE沥青混合料各项性能较为接近,抗疲劳性能变差,但是优于70#沥青混合料。