阿魏菇多糖高产菌株筛选的离子束诱变和复合诱变对比研究

为筛选出阿魏菇菌丝体多糖高产菌株，本文进行了阿魏菇多糖高产菌株筛选的离子束诱变和复合诱变对比研究。尝试用打散的阿魏菇菌丝体为靶材，以离子束注入为诱变手段，通过营养缺陷型初筛和摇瓶发酵复筛，获得了2株阿魏菇多糖高产菌株PFPH-1和PFPH-2。其菌丝体多糖产量分别达到551．80mg，L和659．46mg／L，比出发菌株分别提高了46．55％和75．14％。再分别以PFPH．1和PFPH．2为出发菌株，用紫外线和氯化锂进行复合诱变处理，通过高通量初筛和摇瓶发酵复筛，结果发现，菌株1、9和10号比其出发菌株PFPH-1的多糖产量分别减少了27％、38％和37％；菌株17号比其出发菌株PFPH-2的多糖产量减少了28％。在本研究中，紫外线和氯化锂的复合诱变表现出了一定的负效应。     

食(药)用真菌多糖研究进展

综述了食(药)用真菌多糖的提取工艺、化学结构、生物活性以及食(药)用真菌多糖产品的开发现状,展望了 食(药)用真菌多糖的研发前景。     

鸡腿菇液体深层发酵培养基的优化

通过单因素试验,确定玉米粉、蔗糖为碳源,麸皮、酵母浸粉为氮源;在此基础上,以筛选的碳源、氮源和无机盐KH2PO4和MgSO4为考察因素,以生物量为主要指标利用正交试验优化培养基配方比例,确定鸡腿蘑摇瓶发酵培养基最佳配方为：蔗糖2．0％,酵母浸粉0．5％,玉米粉3％,麸皮4％,KH2PO40．35％,MgSO40．10％,此时干重可达1．83g／100mL。     

低能氮离子注入香菇生物学效应的研究

本文用能量为 15 keV,而注量不同的氮离子注入对武香一号香菇进行诱变,分析了不同注量下菌丝长速,菌丝生物量以及多糖含量的变化,对该低能离子与微生物间的作用规律进行了初步探索,为进一步筛选出多糖高产菌株提供前提.实验结果表明菌丝长速,菌丝生物量及多糖含量随不同注量的变化情况基本遵循"马鞍型曲线".注量小时菌丝日均长速较快,随着注量的增大,菌丝日均长速呈下降趋势,但在1.5×1016cm-2附近,菌丝长速有短暂回升.在注量较小时,随着注量的增加,菌丝生物量及多糖含量先升高后降低,注量为4×1015cm-2时,两者均达到最大值,而后会出现小的波动,注量增加到1.5×1016cm-2时,菌丝生物量及多糖含量又会达到-个较大的峰值.通过发酵动力学分析,研究了香菇液体菌种培养过程中菌丝球数量及形态、摇瓶总重、pH值、多糖含量等指标的变化情况,绘制该菌发酵产糖的过程曲线,这将为诱变前后液体发酵过程的控制提供参数.     

Mutation-Screening of Pleurotus Ferulae with High Temperature Tolerance by Nitrogen Ion Implantation

In order to obtain Pleurotus ferulae with high temperature tolerance, conidiophores of wild type strain ACK were implanted with nitrogen ions in energy of 5 -15 keV and dose of 1.5×10^15 - 1.5 × 10^16 cm^-2, and a mutant CGMCC1763 was isolated subsequently through thermotolerant screening method. It was found that during riper period the surface layer mycelium of the mutant in mushroom bag wasn＇t aging neither grew tegument even above 30℃. The mycelium endurable temperature of the mutant was increased by 5℃ compared to that of the wild type strain. The fruiting bodies growth temperature of the mutant was 18 -22℃ in daytime and 8 -14℃ at night. The highest growth temperature of fruiting bodies of the mutant was increased about 7℃ w.r.t, that of original strain. Through three generations investigations, it was found that the mutant CGMCC1763 was stable with high temperature tolerance.