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原位氮饥饿发酵工艺中梯度补氮对谷氨酰胺合成酶的调控 总被引:4,自引:1,他引:3
The effects of uniform and gradient fed nitrogen on glutamine synthetase (GS), glutamate dehydrogenase(GDH) and glutamate synthase ((K)GAT)were investigated in glutamine production by fermentation of Corynebacterium glutamicum NS611 after 3 h of in-situ nitrogen starvation. It was shown that the strain in the later growth phase entered naturally into in-situ nitrogen starvation by controlling the initial concentration of urea and the biomass was slightly decreased. The pH value reached 6.5 again in the culture system, which confirmed the beginning of nitrogen starvation in the culture system. After 3 h nitrogen starvation the activity of GS was increased over two folds and the time of high activity of GS persisted three folds longer in the gradient fed nitrogen system than that in the normal fed batch. The higher activity of GDH was also maintained. The glutamine production increased by 72 % than the original technology of nitrogen starvation and the time of fermentation was shortened by above 12 h. 相似文献
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建立并完善了谷氨酸棒杆菌NS611生产谷氨酰胺的中心碳代谢网络.研究了菌体生长和生产阶段的代谢流分布,结果说明该菌葡萄糖的代谢以糖酵解途径为主,在产酸阶段丙酮酸羧化反应的代谢流量较大。NADPH不是谷氨酸棒杆菌NS611代谢的限制性因素.研究了氮饥饿处理对代谢流分布的影响,提出要提高谷氨酰胺的产量必须适当保留α-酮戊二酸的向下氧化的功能,以保证能量供给. 相似文献
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谷氨酰胺产生菌NS611的代谢流分析 总被引:3,自引:0,他引:3
建立并完善了谷氨酸棒杆菌NS611生产谷氨酰胺的中心碳代谢网络。研究了菌体生长和生产阶段的代谢流分布,结果说明该菌葡萄糖的代谢以糖酵解途径为主,在产酸阶段丙酮酸羧化反应的代谢流量较大,NADPH不是谷氨酸棒杆菌NS611代谢的限制性因素。研究了氮饥饿处理对代谢流分布的影响,提出要提高谷氨酰胺的产量必须适当保留a-酮戊二酸的向下氧化的功能,以保证能量供给。 相似文献
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