全文获取类型
收费全文 | 645篇 |
免费 | 44篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 7篇 |
综合类 | 9篇 |
化学工业 | 160篇 |
金属工艺 | 23篇 |
机械仪表 | 25篇 |
建筑科学 | 15篇 |
矿业工程 | 1篇 |
能源动力 | 20篇 |
轻工业 | 64篇 |
水利工程 | 2篇 |
石油天然气 | 2篇 |
无线电 | 40篇 |
一般工业技术 | 165篇 |
冶金工业 | 83篇 |
原子能技术 | 1篇 |
自动化技术 | 73篇 |
出版年
2024年 | 1篇 |
2023年 | 11篇 |
2022年 | 8篇 |
2021年 | 47篇 |
2020年 | 20篇 |
2019年 | 22篇 |
2018年 | 24篇 |
2017年 | 27篇 |
2016年 | 24篇 |
2015年 | 18篇 |
2014年 | 29篇 |
2013年 | 29篇 |
2012年 | 42篇 |
2011年 | 52篇 |
2010年 | 36篇 |
2009年 | 30篇 |
2008年 | 34篇 |
2007年 | 27篇 |
2006年 | 21篇 |
2005年 | 16篇 |
2004年 | 11篇 |
2003年 | 10篇 |
2002年 | 10篇 |
2001年 | 5篇 |
2000年 | 7篇 |
1999年 | 9篇 |
1998年 | 17篇 |
1997年 | 12篇 |
1996年 | 13篇 |
1995年 | 7篇 |
1994年 | 16篇 |
1993年 | 9篇 |
1992年 | 6篇 |
1991年 | 6篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 6篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1978年 | 2篇 |
1976年 | 4篇 |
1975年 | 2篇 |
1974年 | 1篇 |
排序方式: 共有690条查询结果,搜索用时 78 毫秒
1.
Gennaro Lettieri Rosaria Notariale Alessia Ambrosino Alfredo Di Bonito Antonella Giarra Marco Trifuoggi Caterina Manna Marina Piscopo 《International journal of molecular sciences》2021,22(4)
Mercury (Hg) is an environmental pollutant that impacts human and ecosystem health. In our previous works, we reported alterations in the properties of Mytilus galloprovincialis protamine-like (PL) proteins after 24 h of exposure to subtoxic doses of toxic metals such as copper and cadmium. The present work aims to assess the effects of 24 h of exposure to 1, 10, and 100 pM HgCl2 on spermatozoa and PL proteins of Mytilus galloprovincialis. Inductively coupled plasma–mass spectrometry indicated accumulation of this metal in the gonads of exposed mussels. Further, RT-qPCR analyses showed altered expression levels of spermatozoa mt10 and hsp70 genes. In Mytilus galloprovincialis, PL proteins represent the major basic component of sperm chromatin. These proteins, following exposure of mussels to HgCl2, appeared, by SDS-PAGE, partly as aggregates and showed a decreased DNA-binding capacity that rendered them unable to prevent DNA damage, in the presence of CuCl2 and H2O2. These results demonstrate that even these doses of HgCl2 exposure could affect the properties of PL proteins and result in adverse effects on the reproductive system of this organism. These analyses could be useful in developing rapid and efficient chromatin-based genotoxicity assays for pollution biomonitoring programs. 相似文献
2.
Alessandro Caputo Angela DArdia Francesco Sabbatino Caterina Picariello Chiara Ciaparrone Pio Zeppa Antonio DAntonio 《International journal of molecular sciences》2021,22(9)
The current standard of care for advanced non-small-cell lung cancer is based on detecting actionable mutations that can benefit from targeted therapy. Comprehensive genetic tests can have long turn-around times, and because EGFR mutations are the most prevalent actionable mutation, a quick detection would enable a prompt initiation of targeted therapy. Furthermore, the scarcity of diagnostic material means that sometimes only cytologic material is available. The Idylla™ EGFR assay is a real-time PCR–based method able to detect 51 EGFR mutations in 2.5 h. Idylla is validated for use only on FFPE sections, but some researchers described their experiences with cytological material. We reviewed the relevant literature, finding four articles describing 471 cases and many types of cytological input material: smears, cell-block sections, suspensions, and extracted DNA. The sensitivity, specificity, and limit of detection appear comparable to those obtained with histological input material, with one exception: the usage of scraped stained smears as input may reduce the accuracy of the test. In conclusion, usage of cytological material as input to the Idylla EGFR test is possible. A workflow where common mutations are tested first and fast, leaving rarer mutations for subsequent comprehensive profiling, seems the most effective approach. 相似文献
3.
Aseem Grover Reshma Sinha Divya Jyoti Caterina Faggio 《Microscopy research and technique》2022,85(5):1976-1989
Electron microscope (EM) was developed in 1931 and since then microscopical examination of both the biological and non-biological samples has been revolutionized. Modifications in electron microscopy techniques, such as scanning EM and transmission EM, have widened their applicability in the various sectors such as understanding of drug toxicity, development of mechanism, criminal site investigation, and characterization of the nano-molecule. The present review summarizes its role in important aspects such as toxicity assessment and disease diagnosis in special reference to SARS-COV2. In the biological system, EM studies have elucidated the impact of toxicants at the ultra-structural level in various tissue in conformity to physiological alterations. Thus, EM can be concluded as an important tool in toxicity assessment and disease prognosis. 相似文献
4.
Shanmuganathan Archunan Maharajan Athisuyambulingam Kumara Perumal Pradhoshini Narayanaswamy Yelappu Ganapiriya viswambaran Muthukumaravel Kannayiram Marckasagayam Priyadharshini Mohamed Saiyad Musthafa Caterina Faggio 《Microscopy research and technique》2022,85(4):1355-1362
Accumulation of pollutants in the aquatic system has a high impact on the reproductive physiology of crustaceans. The objective of the present study was to assess the possible histopathological effects of combined chlorpyrifos and cypermethrin (nurocombi) exposure on reproductive tissue in male freshwater crab Paratelphusa jacquemontii using light and electron microscopy. The testis of experimental crabs showed disorganization of testicular lobules, increased inters cellular space, necrosis, and cellular damage in both germinal cells and Sertoli cells. The treated vas deferens exhibited epithelial degeneration, misshaped spermatophores, decline in the number of spermatophores, and dehiscence of spermatophore wall. These clinical manifestations expressed in crabs following the exposure of nurocombi significantly reduce the testicular activity and substantially inhibits the seminal secretions, which ultimately lead to impairment of reproduction. 相似文献
5.
Buzzi Maria Claudia Buzzi Marina Perrone Erico Senette Caterina 《Universal Access in the Information Society》2019,18(4):891-907
Universal Access in the Information Society - Today, ICT technology offers mobile, customized user-friendly environments to support learning and stimulate an individual’s potential. However,... 相似文献
6.
Andrea Angius Antonio Mario Scanu Caterina Arru Maria Rosaria Muroni Vincenzo Rallo Giulia Deiana Maria Chiara Ninniri Ciriaco Carru Alberto Porcu Giovanna Pira Paolo Uva Paolo Cossu-Rocca Maria Rosaria De Miglio 《International journal of molecular sciences》2021,22(4)
Colorectal cancer (CRC) is a leading cause of cancer death worldwide, and about 20% is metastatic at diagnosis and untreatable. Increasing evidence suggests that the heterogeneous nature of CRC is related to colorectal cancer stem cells (CCSCs), a small cells population with stemness behaviors and responsible for tumor progression, recurrence, and therapy resistance. Growing knowledge of stem cells (SCs) biology has rapidly improved uncovering the molecular mechanisms and possible crosstalk/feedback loops between signaling pathways that directly influence intestinal homeostasis and tumorigenesis. The generation of CCSCs is probably connected to genetic changes in members of signaling pathways, which control self-renewal and pluripotency in SCs and then establish function and phenotype of CCSCs. Particularly, various deregulated CCSC-related miRNAs have been reported to modulate stemness features, controlling CCSCs functions such as regulation of cell cycle genes expression, epithelial-mesenchymal transition, metastasization, and drug-resistance mechanisms. Primarily, CCSC-related miRNAs work by regulating mainly signal pathways known to be involved in CCSCs biology. This review intends to summarize the epigenetic findings linked to miRNAome in the maintenance and regulation of CCSCs, including their relationships with different signaling pathways, which should help to identify specific diagnostic, prognostic, and predictive biomarkers for CRC, but also develop innovative CCSCs-targeted therapies. 相似文献
7.
8.
Tim Helbing Caterina Carraro Alexander Francke Dr. Alice Sosic Michele De Franco Prof. Dr. Valentina Gandin Prof. Dr. Richard Göttlich Prof. Dr. Barbara Gatto 《ChemMedChem》2020,15(21):2040-2051
In this study, we describe the synthesis and biological evaluation of a set of bis-3-chloropiperidines (B−CePs) containing rigid aromatic linker structures. A modification of the synthetic strategy also enabled the synthesis of a pilot tris-3-chloropiperidine (Tri-CeP) bearing three reactive meta-chloropiperidine moieties on the aromatic scaffold. A structure–reactivity relationship analysis of B−CePs suggests that the arrangement of the reactive units affects the DNA alkylating activity, while also revealing correlations between the electron density of the aromatic system and the reactivity with biologically relevant nucleophiles, both on isolated DNA and in cancer cells. Interestingly, all aromatic 3-chloropiperidines exhibited a marked cytotoxicity and tropism for 2D and 3D cultures of pancreatic cancer cells. Therefore, the new aromatic 3-chloropiperidines appear to be promising contenders for further development of mustard-based anticancer agents aimed at pancreatic cancers. 相似文献
9.
Carina A. Lämmle Adam Varady Thorsten G. Müller Dr. Caterina Sturtzel Michael Riepl Bettina Mathes Jenny Eichhorst Dr. Anje Sporbert Dr. Martin Lehmann Prof. Dr. Hans-Georg Kräusslich Dr. Martin Distel Dr. Johannes Broichhagen 《Chembiochem : a European journal of chemical biology》2021,22(3):548-556
Selective targeting of DNA by means of fluorescent labeling has become a mainstay in the life sciences. While genetic engineering serves as a powerful technique and allows the visualization of nucleic acid by using DNA-targeting fluorescent fusion proteins in a cell-type- and subcellular-specific manner, it relies on the introduction of foreign genes. On the other hand, DNA-binding small fluorescent molecules can be used without genetic engineering, but they are not spatially restricted. Herein, we report a photocaged version of the DNA dye Hoechst33342 (pcHoechst), which can be uncaged by using UV to blue light for the selective staining of chromosomal DNA in subnuclear regions of live cells. Expanding its application to a vertebrate model organism, we demonstrate uncaging in epithelial cells and short-term cell tracking in vivo in zebrafish. We envision pcHoechst as a valuable tool for targeting and interrogating DNA with precise spatiotemporal resolution in living cells and wild-type organisms. 相似文献
10.
In this paper, different arrangements of Kelvin–Voigt elements are implemented in an admittance matrix model to investigate the transient modeling of water distribution systems. The viscoelastic parameters are calibrated by experimental data acquired during a transient in a branched system of three HDPE pipes at the Water Engineering Laboratory of the University of Perugia, Italy. The dependence of the viscoelastic parameters on the branch characteristic time is investigated. Models with a single set of elements for all branches are compared with models with different sets of elements for each branch. When the same number of parameters is used, the former perform better than the latter. No evident correlation is found between the retardation times of the viscoelastic parameters and the branch characteristic times. The use of two measurement sections instead of one does not modify the calibration results significantly. 相似文献