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排序方式: 共有236条查询结果,搜索用时 198 毫秒
1.
Ewelina Bik Lukasz Mateuszuk Jagoda Orleanska Malgorzata Baranska Stefan Chlopicki Katarzyna Majzner 《International journal of molecular sciences》2021,22(5)
Chloroquine (CQ) is an antimalarial drug known to inhibit autophagy flux by impairing autophagosome–lysosome fusion. We hypothesized that autophagy flux altered by CQ has a considerable influence on the lipid composition of endothelial cells. Thus, we investigated endothelial responses induced by CQ on human microvascular endothelial cells (HMEC-1). HMEC-1 cells after CQ exposure were measured using a combined methodology based on label-free Raman and fluorescence imaging. Raman spectroscopy was applied to characterize subtle chemical changes in lipid contents and their distribution in the cells, while the fluorescence staining (LipidTox, LysoTracker and LC3) was used as a reference method. The results showed that CQ was not toxic to endothelial cells and did not result in the endothelial inflammation at concentrations of 1–30 µM. Notwithstanding, it yielded an increased intensity of LipidTox, LysoTracker, and LC3 staining, suggesting changes in the content of neutral lipids, lysosomotropism, and autophagy inhibition, respectively. The CQ-induced endothelial response was associated with lipid accumulation and was characterized by Raman spectroscopy. CQ-induced autophagosome accumulation in the endothelium is featured by a pronounced alteration in the lipid profile, but not in the endothelial inflammation. Raman-based assessment of CQ-induced biochemical changes offers a better understanding of the autophagy mechanism in the endothelial cells. 相似文献
2.
Nicki Frederiksen Assoc. Prof. Paul R. Hansen Dr. Dorota Zabicka Magdalena Tomczak Malgorzata Urbas Dr. Ilona Domraceva Prof. Fredrik Björkling Assoc. Prof. Henrik Franzyk 《ChemMedChem》2020,15(24):2544-2561
The influence of hydrophobicity on antibacterial activity versus the effect on the viability of mammalian cells for peptide/peptoid hybrids was examined for oligomers based on the cationic Lys-like peptoid residue combined with each of 28 hydrophobic amino acids in an alternating sequence. Their relative hydrophobicity was correlated to activity against both Gram-negative and Gram-positive species, human red blood cells, and HepG2 cells. This identified hydrophobic side chains that confer potent antibacterial activity (e. g., MICs of 2–8 μg/mL against E. coli) and low toxicity toward mammalian cells (<10 % hemolysis at 400 μg/mL and IC50>800 μg/mL for HepG2 viability). Most peptidomimetics retained activity against drug-resistant strains. These findings corroborate the hypothesis that for related peptidomimetics two hydrophobicity thresholds may be identified: i) it should exceed a certain level in order to confer antibacterial activity, and ii) there is an upper limit, beyond which cell selectivity is lost. It is envisioned that once identified for a given subclass of peptide-like antibacterials such thresholds can guide further optimisation. 相似文献
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Mengting Guo Chongyun Shao Yang Zhang Jingbo Yu Yan Jiao Malgorzata Guzik Georges Boulon Jinjun Ren Lili Hu 《Journal of the American Ceramic Society》2020,103(8):4275-4285
The homogeneous Yb3+/Al3+/B3+-co-doped silica glasses were prepared via a sol-gel method. The impact of B2O3 addition on the physical and optical properties and network structure was systematically studied. The network structure was investigated by the Fourier Transform Infrared (FT-IR), Raman spectra, and Solid State Nuclear Magnetic Resonance (SSNMR). Herein, B2O3 addition can continuously decrease the refractive index and density. When B2O3 is lower than 2 mol%, B2O3 addition can obviously decrease the scalar crystal field parameters, Yb3+ asymmetry degree, Yb3+ cross-sections, due to the generation of Yb–O–B bonds at the cost of partial Yb–O–Al/Si ones. When B2O3 is more than 2 mol%, FT-IR, Raman spectra, and SSNMR results indicate that further increased B atoms prefer to connect with Si and Al rather than Yb. Consequently, the above parameters are basically unchanged. Based on the results, an intuitive model of structure and properties evolution during the substitution of SiO2 by B2O3 has been established. 相似文献
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Grzegorz Mikusiński Malgorzata Blicharska Hans Antonson Marianne Henningsson Görgen Göransson Per Angelstam 《Landscape Research》2013,38(3):384-393
Implementation of the European Landscape Convention requires new tools that link ecological, social and cultural dimensions in practical planning. Here, we propose connectivity as a conceptual tool to include different dimensions into landscape and spatial planning. We present a short review of the connectivity concept in relation to ecological, social and cultural dimensions and illustrate it by examples from a real landscape planning case. 相似文献
7.
Katarzyna M. Terlikowska Anna M. Witkowska Malgorzata E. Zujko Bozena Dobrzycka Slawomir J. Terlikowski 《International journal of molecular sciences》2014,15(12):21703-21722
Recent findings on the molecular basis of ovarian cancer development and progression create new opportunities to develop anticancer medications that would affect specific metabolic pathways and decrease side systemic toxicity of conventional treatment. Among new possibilities for cancer chemoprevention, much attention is paid to curcumin—A broad-spectrum anticancer polyphenolic derivative extracted from the rhizome of Curcuma longa L. According to ClinicalTrials.gov at present there are no running pilot studies, which could assess possible therapeutic benefits from curcumin supplementation to patients with primary epithelial ovarian cancer. Therefore, the goal of this review was to evaluate potential preclinical properties of curcumin and its new analogues on the basis of in vivo and in vitro ovarian cancer studies. Curcumin and its different formulations have been shown to display multifunctional mechanisms of anticancer activity, not only in platinum-resistant primary epithelial ovarian cancer, but also in multidrug resistant cancer cells/xenografts models. Curcumin administered together with platinum-taxane chemotherapeutics have been reported to demonstrate synergistic effects, sensitize resistant cells to drugs, and decrease their biologically effective doses. An accumulating body of evidence suggests that curcumin, due to its long-term safety and an excellent profile of side effects should be considered as a beneficial support in ovarian cancer treatment strategies, especially in patients with platinum-resistant primary epithelial recurrent ovarian cancer or multidrug resistant disease. Although the prospect of curcumin and its formulations as anticancer agents in ovarian cancer treatment strategy appears to be challenging, and at the same time promising, there is a further need to evaluate its effectiveness in clinical studies. 相似文献
8.
An alternative RP‐HPLC method for the separation and determination of tocopherol and tocotrienol homologues as butter authenticity markers: A comparative study between two European countries 下载免费PDF全文
9.
Dorota Rutkowska-Zbik Agnieszka Drzewiecka-Matuszek Malgorzata Witko 《Topics in Catalysis》2014,57(10-13):946-952
The present brief review aims at elucidation of the parameters affecting the properties and reactivity of high valent iron(IV) oxo porphyrin complexes, being models of compound II reactive intermediate known from biochemistry of cytochromes and peroxidases. The stress is put on the influence of the axial ligand and porphyrin ring substituents, with special attention on implications for their catalytic activity. 相似文献
10.
Akylbek Sydykov Himal Luitel Argen Mamazhakypov Malgorzata Wygrecka Kabita Pradhan Oleg Pak Aleksandar Petrovic Baktybek Kojonazarov Norbert Weissmann Werner Seeger Friedrich Grimminger Hossein Ardeschir Ghofrani Djuro Kosanovic Ralph Theo Schermuly 《International journal of molecular sciences》2020,21(23)
Although the response of the right ventricle (RV) to the increased afterload is an important determinant of the patient outcome, very little is known about the underlying mechanisms. Mast cells have been implicated in the pathogenesis of left ventricular maladaptive remodeling and failure. However, the role of mast cells in RV remodeling remains unexplored. We subjected mast cell-deficient WBB6F1-KitW/W-v (KitW/KitW-v) mice and their mast cell-sufficient littermate controls (MC+/+) to pulmonary artery banding (PAB). PAB led to RV dilatation, extensive myocardial fibrosis, and RV dysfunction in MC+/+ mice. In PAB KitW/KitW-v mice, RV remodeling was characterized by minimal RV chamber dilatation and preserved RV function. We further administered to C57Bl/6J mice either placebo or cromolyn treatment starting from day 1 or 7 days after PAB surgery to test whether mast cells stabilizing drugs can prevent or reverse maladaptive RV remodeling. Both preventive and therapeutic cromolyn applications significantly attenuated RV dilatation and improved RV function. Our study establishes a previously undescribed role of mast cells in pressure overload-induced adverse RV remodeling. Mast cells may thus represent an interesting target for the development of a new therapeutic approach directed specifically at the heart. 相似文献