A role of sea buckthorn on Alzheimer’s disease

Evidence suggests that diets rich in antioxidants reduce the risk factors of Alzheimer’s disease (AD). Hippophae rhamnoides, commonly known as sea buckthorn (SB), is rich in antioxidants which could have direct effects on amyloid-beta (Aβ) levels and consequently influence AD pathogenesis. In this study, sea buckthorn powder (SBP) was administered at varying concentrations (0.6, 0.9, 1.2, 1.5 and 1.8 µg mL⁻¹) to cell cultures (BE(2)-M17) with 20 mm Aβ for 72 h. MTS test indicated that SB significantly increased cell viability in Aβ-induced cells up to 95%. Results of Western blot showed maximum 38% inhibition of Aβ compared to the control (Aβ only). ELISA demonstrated significantly lower amyloid-β level (6672 pg mL⁻¹) than the control (10189 pg mL⁻¹). Images of AFM further confirmed the presence of low quantity of amyloid beta in SBP-treated cells. These findings suggest that SB warrants further investigation as potential therapeutic agent in the treatment of AD.     

Local structure and photocatalytic property of sol–gel synthesized ZnO doped with transition metal oxides

ZnO nanoparticles doped with up to 5 at% of Co and Mn were prepared using a co-precipitation method. The location of dopant ions and the effect of doping on the photocatalytic activity were investigated. The crystal structure of nanoparticles and local atomic arrangements around dopant ions were analyzed by X-ray absorption spectroscopy. The results showed that the Co ions substituted the Zn ions in the ZnO wurtzite phase structure and induced lattice shrinkage, while Mn ions were not completely incorporated in the crystal lattice. The photocatalytic activity under simulated sunlight was characterized by the decomposition of Rhodamine B dye molecules. It was revealed that Co-doping strongly reduced the photocatalytic activity but Mn-doping showed a weaker effect on the reduction of the photoactivity.     

Development of disease management recommendations for the durian and jackfruit industries in the Philippines using farmer participatory research

Promoting the adoption of improved technologies among smallholder farmers is a challenge faced by agricultural scientists and extension personnel around the world. In the southern Philippines, Phytophthora diseases severely affect the yields of durian and jackfruit. A limited understanding of these diseases among farmers and extension professionals compromises their effective management and significantly reduces yields. Recommended management interventions were rarely validated in field trials. A participatory research approach was used to collect information on current farming practices, identify industry constraints, and to develop and promote management strategies for smallholder durian and jackfruit growers. Farmers identified the development of effective disease management strategies as the highest priority so information on the biology, epidemiology and management of the suspected pathogen, Phytophthora, and other pests and diseases, was presented and discussed. Participants were then asked to propose and design research trials to diagnose the pathogen and to test simple management interventions on their crops. The trials were established and managed by participants and monitored by the group of growers, researchers and extension staff over 3 years. Responsibility for the on-farm trials encouraged participants to become directly involved in the research process, improving their skills in developing and critically assessing solutions for management of their orchards, and in decision-making processes. Results from the Participatory Action Research (PAR) trials were subjected to simple benefit-cost analyses and used to formulate a series of low-, medium- and high-input disease management packages. The low-input options included sanitation and manual pruning and weeding, and other activities requiring relatively low levels of time and financial investment. Medium- and high-input options required greater investment of time, resources and finances and included application of soil amendments, water management through construction of drainage channels and mounding, and the application of pesticides. The availability of a range of options facilitates the adoption of improved management by farmers from diverse backgrounds, resources and capacity.     

Characterization of Apo-Form Selective Inhibition of Indoleamine 2,3-Dioxygenase**

Indoleamine-2,3-dioxygenase 1 (IDO1) is a heme-containing enzyme that catalyzes the rate-limiting step in the kynurenine pathway of tryptophan (TRP) metabolism. As it is an inflammation-induced immunoregulatory enzyme, pharmacological inhibition of IDO1 activity is currently being pursued as a potential therapeutic tool for the treatment of cancer and other disease states. As such, a detailed understanding of the mechanism of action of IDO1 inhibitors with various mechanisms of inhibition is of great interest. Comparison of an apo-form-binding IDO1 inhibitor (GSK5628) to the heme-coordinating compound, epacadostat (Incyte), allows us to explore the details of the apo-binding inhibition of IDO1. Herein, we demonstrate that GSK5628 inhibits IDO1 by competing with heme for binding to a heme-free conformation of the enzyme (apo-IDO1), whereas epacadostat coordinates its binding with the iron atom of the IDO1 heme cofactor. Comparison of these two compounds in cellular systems reveals a long-lasting inhibitory effect of GSK5628, previously undescribed for other known IDO1 inhibitors. Detailed characterization of this apo-binding mechanism for IDO1 inhibition might help design superior inhibitors or could confer a unique competitive advantage over other IDO1 inhibitors vis-à-vis specificity and pharmacokinetic parameters.     

Psychological resilience and dysfunction among hospitalized survivors of the SARS epidemic in Hong Kong: A latent class approach.

Objective: To examine trajectories of psychological functioning using latent class analysis on a sample of hospitalized survivors of the 2003 severe acute respiratory syndrome (SARS) epidemic in Hong Kong. Design: A longitudinal study of 997 survivors, recruited from among 1,331 individuals hospitalized for SARS, were interviewed at 6, 12, and 18 months after hospitalization. Main Outcome Measures: Psychological and physical functioning at each time point was measured using the 12-item Medical Outcome Study Short-Form Health Survey (SF-12). Results: Four latent classes were identified-chronic dysfunction, delayed dysfunction, recovery, and resilience. All groups had better physical health than the chronic group. Resilient and recovered individuals had greater social support and less SARS-related worry, and resilient individuals were more likely to be male. The resilient group also had greater social support than the delayed group and better physical functioning than the recovered group. Conclusion: This study demonstrated that longitudinal outcome trajectories following a major health-threat event in an Asian sample bear close resemblance to prototypical trajectories observed in trauma studies using Western samples. Unique predictors of the trajectories included factors observed in previous studies, such as social support, as well as factors of particular relevance to a major disease outbreak, such as SARS-related worry. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Psychological separation, attachment security, vocational self-concept crystallization, and career indecision: A structural equation analysis.

Structural equation modeling was used to test theoretically based models in which psychological separation and attachment security variables were related to career indecision and those relations were mediated through vocational self-concept crystallization. In contrast to previous studies, which have found either weak or no support for a relation between separation or attachment security variables and career indecision, results based on a sample of 350 college students indicated that some components of separation and attachment security did relate to career indecision in a theoretically supportable direction. Results also revealed that regardless of whether global or component career indecision measures were used, separation and attachment security effects on indecision were at least partially mediated through vocational self-concept crystallization. The strongest mediated relations were observed for the effects of attachment anxiety and maternal separation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Relationship between perceptual style and reactivity to motion.

The relationship between the perceptual-style dimension of field dependence-independence, as measured by the rod-and-frame test (RFT) and the group Hidden Figures Test, and motion disturbance, as evidenced by a version of the Brief Vestibular Disorientation Test (BVDT) was investigated in 83 naval aviation students. Significant correlations were found between RFT scores and BVDT scores. Field dependence was significantly associated with disturbance by the motion stimulus of the test. Apparent contradictions of the present findings with those involving simulator sickness are discussed. (25 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Correlates of employee satisfaction with stock ownership: Who likes an ESOP most?

In this study, we examined the correlates of individual employee satisfaction with stock ownership in a sample of 37 employee stock ownership plan (ESOP) companies. The results indicated that ESOP satisfaction is a function of five factors: (a) characteristics of the company ESOP, (b) employee status within the ESOP, (c) employee values, (d) interactions between employee and ESOP characteristics, and (e) employees' general attitude toward the organization as a whole (organizational commitment). Together, these five factors accounted for 58% of the variance in ESOP satisfaction. The results both support and extend previous employee stock ownership research and theory. (PsycINFO Database Record (c) 2010 APA, all rights reserved)